Production of peptide antibiotics by Bacillus sp: GU 057 indigenously isolated from saline soil

Amin, Adnan; Khan, M. A. K. G.; Ehsanullah, Malik; Haroon, Uzma; Azam, Sheikh Muhammad; Hameed, Abdul

doi:10.1590/s1517-83822012000400015

Cited by 35 publications

(30 citation statements)

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“…Bacillus spp. makes antibiotic compound i.e colistin and polymixin, that are effective against Gram-negative bacteria (Amin et al, 2012). A research conducted by Navinchandran et al (2014) revealed that extracellular enzymes that are produces by B. cereus as probiotics (supplemented through shrimp feed) can increase both immunology parameters and growth of the host.…”

Section: Discussionmentioning

confidence: 99%

Multispecies probiotics applications through feed addition on cultured tilapia to prevent streptococcosis

Destianingrum¹,

Yuhana²,

Lusiastuti³

2017

Jurnal Akuakultur Indonesia

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Streptococcosis is one of the diseases that often arise and can cause death by 100% in tilapia fish farming. This study aimed to determine the most optimal probiotics combination of cells Bacillus cereus P22, Bacillus subtillis ND2 and Staphylococcus lentus L1k through the feed for streptococcosis prevention. The experimental fish with initial body weight of (13.06±0.18 g), were randomly distributed at 25 fish per 60 L water, and reared for 21 days. The fish fed with supplemented feed (except controls) with FR 3%, about three times a day, for 14 days.

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Section: Discussionmentioning

confidence: 99%

Multispecies probiotics applications through feed addition on cultured tilapia to prevent streptococcosis

Destianingrum¹,

Yuhana²,

Lusiastuti³

2017

Jurnal Akuakultur Indonesia

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“…were transferred. The agar plates were incubated at 37 o C for 24hr and the zones of inhibition around each well were measured [1] Determining the optimal conditions for antibacterial activity of Bacillus spp.…”

Section: Screening Antibacterial Activity Produced By Bacillus Sppmentioning

confidence: 99%

“…The plates were conserved at room temperature for 1hr., and then they were incubated at optimal cultural conditions. After the incubation period, the inhibitory zones (mm) were measured [1] Antibiofilm analysis of the extracts The biofilm inhibition assay was described by [16]. Following incubation for 24hr., the biofilm was allowed to be formed.…”

Section: Applications Of Purified Extracts As Antibacterial Antibiofmentioning

confidence: 99%

“…Antibiotics, naturally formed by microorganisms are responsible for the control and regulation of microbial population in soil, compost and water. The majority of antibiotics are toxic in nature and only a few are used in medicine for treatment of multiple diseases due to their high safety [1]. Bacterial secondary metabolites or bioactive compounds have structural diversity obtained from natural sources that enhance the biological activity of the host.…”

Section: Introductionmentioning

confidence: 99%

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The Biological Activity of Some Extracted Proteins from Bacillus spp. Isolated from Soil Against Pathogenic Bacteria

Ibrahim

Zaki

2020

MJS

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Forty soil samples were collected from different regions in Baghdad city. Among 52 isolate, only 38 isolates (73.1 %) belong to Bacillus spp. All bacterial isolates have been submitted to the cultural, microscopical, biochemical examination and VITEK 2 System, Bacillus cereus was predominantly found 21 (55.3%) followed by Bacillus subtilis 11 (28.9%), then Bacillus amyloliquefaciens was 6 (15.8%). Bacillus cereus (B1) and Bacillus subtilis (B16) isolates were with the highest level with the antibacterial activity when selected. Extracellular proteins were extracted and purified with ammonium sulfate (80%) saturation, then submitted to Sepharose-6B gel filtration column. SDS-polyacrylamide gel electrophoresis was used to determine the molecular weights of proteins, and one band appeared to each of Bacillus cereus and Bacillus subtilis with molecular weight 68 and 50 KDa respectively. The results revealed that higher effect of antibacterial activity on Gram positive bacteria, and moderate on Gram negative bacteria except P.aeruginosa with no effect. The antibiofilm effect of the extract found higher effective against S.pyogens, moderate effect against each of (S.aureus, E.coli, S. marcescens, K. spp) and no effect against P.aeruginosa. The results of cytotoxicity showed that the purified extract was a higher cytotoxic effect on breast cancer MCF7 cells at 1000µg/ml concentration, while the crude extract had a higher cytotoxic effect on breast cancer MCF7 cells at 100µg/ml concentration.

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“…Similarly, epibiotic bacteria from marine sponge Petrosia ficiformis (Chelossi et al 2004), bacteria from marine mollusc Anadara broughtani from the sea of Japan (Romanenko et al 2008), marine bacteria from Sydney Harbour (Wilson et al 2010), marine bacteria in biofilms (Wilson et al 2011) and sponge endosymbiotic bacteria at Agatti Island, Lakshadweep Archipelago (Mohan et al 2016), heterotrophic bacteria from Palk Bay sediments (Nithya and Pandian 2010), bacteria isolated from saline soil (Amin et al 2012), and from soil (Abbas et al 2014) also showed antibacterial activity.…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia

et al. 2019

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Abstract. Ambarwati A, Wahyuono S, Moeljopawiro S, Sembiring L, Yuwono T. 2019. Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia. Biodiversitas 20: 2206-2212. A study has been conducted to investigate the activity of bacteria isolated from the rhizosphere of Purple Nut Sedge (Cyperus rotundus) from Cemoro Sewu Plateu, Indonesia as antimicrobial agent and the molecular relatedness between the isolates with other bacteria based on phylogenetic tree analysis. A total of six bacteria were obtained and tested for their capability to inhibit the growth of test organisms using agar block method and characterized molecularly using 16S rDNA sequence analysis. It was observed that the six isolates demonstrated the ability to inhibit at least one of four test microorganisms growth, with a diameter of inhibition zone ranging from 8-35 mm. The CRC32 isolate was the best isolate in inhibiting Staphylococcus aureus ATCC 25923 with a diameter inhibition zone of 26 mm (strong), while isolate CRA8 was the best anti-Candida (showing 35 mm of inhibition zone). The phylogenetic tree analysis resulted in two genera, four isolates belonging to Bacillus, while the other two isolates were known related to Paenibacillus genus. BLAST analysis on the CRC 32 isolate revealed that it is closely related with Paenibacillus peroriae strain 3763 with 99% sequence similarities and CRA8 isolates demonstrated the highest similarity with Paenibacillus sonchi strain X19-5 (97%). The results of this study demonstrated that the bacterial isolates from plant rhizosphere of Cemoro Sewu Plateau were the members of the Bacillus and Paenibacillus genera and demonstrated the potential as antimicrobial agent.

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Production of peptide antibiotics by Bacillus sp: GU 057 indigenously isolated from saline soil

Cited by 35 publications

References 1 publication

Multispecies probiotics applications through feed addition on cultured tilapia to prevent streptococcosis

Multispecies probiotics applications through feed addition on cultured tilapia to prevent streptococcosis

The Biological Activity of Some Extracted Proteins from Bacillus spp. Isolated from Soil Against Pathogenic Bacteria

Antimicrobial activities and phylogenetic study of bacteria associated with Cyperus rotundus rhizosphere from Cemoro Sewu Plateau, Indonesia

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