Production of oxytetracycline by immobilized Streptomyces rimosus cells in calcium alginate gels

Abstract

Streptomyces rimosus Pfizer 18234-2 cells were immobilized in calcium alginate and used for the production of oxytetracycline. The influence of the incubation period, alginate concentration and storage in CaCI, were investigated. From the results of the repeated batch fermentations of the shake flasks, a good level of antibiotic was maintained for a period of about 28 days using 4% calcium alginate. The cell leakage and cell concentration inside the beads were affected by the alginate concentration and storage in CaCl, solution.

Optimization of pullulan production using Ca‐alginate‐immobilized Aureobasidium pullulans by response surface methodology

“…Cell leakage in Ca‐alginate beads has been observed by various researchers 9, 18, 21, 30, 31. In this study, cell leakage was also observed.…”

“…They did not find a remarkable difference when the concentration of alginate ranged from 3% to 5%. Farid et al 31 investigated the production of oxytetracycline by Ca‐alginate‐immobilized Streptomyces rimosus cells and found the highest level of antibiotic using 4% Ca‐ alginate. Mundra et al 38 used RSM for the optimization of palatinose production using Ca‐alginate‐immobilized Erwinia rhapontici cells and found the highest concentration of palatinose using an alginate concentration of 5%.…”

Immobilization of Bacilli in Various Matrices to Enhance the Utilization of Keratinase and its Comparison

“…Immobilized cells have been used for production of aminoacids, antibiotics, organic acids, enzymes etc. erythromycin production by streptomyces erythreus and oxytetracycline production by streptomyces rimosus cells immobilized in 4% calcium alginate were efficiently recycled 7-12 batches spanning for about 28-30days thereby enhancing productivity of antibiotics (Bandyopadhyay et al, 1993;Farid et al, 1994). Immobilized Serratia marcescens and Myxococcus xanthus in calcium alginate beads had been used for protease production (Vuillemard et al, 1988).. Immobilization of Bacillus sp.…”

Production of alkaline protease with immobilized cells of bacillus subtilis PE-11 in various matrices by entrapment technique

“…They could conduct repeated batch fermentation successfully (each batch 48 hours) for 12 batches (30 days). Similarly, Farid et al 31 reported that a good level of oxytetracycline was produced for a period of 28 days (7 batches) using Streptomyces rimosus cells immobilized in 4% calcium alginate.…”