Production of osteocalcin by human bone cells in vitro. Effects of 1,25(OH)2D3, 24,25(OH)2D3, parathyroid hormone, and glucocorticoids

Over the past few decades, significant progress has been made with respect to new concepts about the pathogenesis of osteoarthritis (OA). This article summarises some of the knowledge we have today on the involvement of the subchondral bone in OA. It provides substantial evidence that changes in the metabolism of the subchondral bone are an integral part of the OA disease process and that these alterations are not merely secondary manifestations, but are part of a more active component of the disease. Thus, a strong rationale exists for therapeutic approaches that target subchondral bone resorption and/or formation, and data evaluating the drugs targeting bone remodelling raise the hope that new treatment options for OA may become available.

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“…Since PTH is a negative regulator of collagen synthesis, this could then lead to altered type I collagen production in OA bone tissue [83,84].…”

Section: Other Factorsmentioning

confidence: 99%

Targeting subchondral bone for treating osteoarthritis: what is the evidence?

Tat

Lajeunesse

Pelletier

et al. 2010

Best Practice & Research Clinical Rheumatology

153

121

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“…The bone fragments were immediately minced and then cultured in DMEM/F12 Ham (Sigma, St. Louis, MO) with 15% fetal bovine serum (FBS) and 100 units/mL penicillin G and 100 mg/mL of streptomycin sulfate. 15 Cells were allowed to grow out of the bone chips for 2-3 weeks. Then the chips were removed and the cultures were allowed to become almost confluent.…”

Section: Cell Culturementioning

confidence: 99%

Therapeutic touch affects DNA synthesis and mineralization of human osteoblasts in culture

Jhaveri

Walsh

Wang

et al. 2008

Journal Orthopaedic Research

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Complementary and alternative medicine (CAM) techniques are commonly used in hospitals and private medical facilities; however, the effectiveness of many of these practices has not been thoroughly studied in a scientific manner. Developed by Dr. Dolores Krieger and Dora Kunz, Therapeutic Touch is one of these CAM practices and is a highly disciplined five-step process by which a practitioner can generate energy through their hands to promote healing. There are numerous clinical studies on the effects of TT but few in vitro studies. Our purpose was to determine if Therapeutic Touch had any effect on osteoblast proliferation, differentiation, and mineralization in vitro. TT was performed twice a week for 10 min each on human osteoblasts (HOBs) and on an osteosarcoma-derived cell line, SaOs-2. No significant differences were found in DNA synthesis, assayed by [ 3 H]-thymidine incorporation at 1 or 2 weeks for SaOs-2 or 1 week for HOBs. However, after four TT treatments in 2 weeks, TT significantly ( p ¼ 0.03) increased HOB DNA synthesis compared to controls. Immunocytochemistry for Proliferating Cell Nuclear Antigen (PCNA) confirmed these data. At 2 weeks in differentiation medium, TT significantly increased mineralization in HOBs ( p ¼ 0.016) and decreased mineralization in SaOs-2 ( p ¼ 0.0007), compared to controls. Additionally, Northern blot analysis indicated a TT-induced increase in mRNA expression for Type I collagen, bone sialoprotein, and alkaline phosphatase in HOBs and a decrease of these bone markers in SaOs-2 cells. In conclusion, Therapeutic Touch appears to increase human osteoblast DNA synthesis, differentiation and mineralization, and decrease differentiation and mineralization in a human osteosarcoma-derived cell line. ß

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“…Primary human osteoblastic cells were isolated from pieces of human trabecular bone obtained from a patient (male 63-years-old) undergoing a total hip arthroplasty as previously described [28]. The pieces of bone were minced into 1 mm 3 pieces, washed three times with sterile PBS, seeded into 25 cm 2 tissue culture flasks and finally cultured at 37 C and 5% CO 2 in Dulbecco's Modified Eagle Medium (Sigma, Buchs, Switzerland) containing 10% of fetal bovine serum (Sigma), and 1% of PSF (100 Â , 10,000 U/ml penicillin, 10,000 mg/ml strepzin, 25 mg/ml fungizone) (GibcoBRL, New York, USA).…”

Section: Cellsmentioning

confidence: 99%

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

Pioletti

Kottelat

2004

Biomaterials

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Orthopedic implant failures are often associated with peri-implant osteolysis. Particles generated from the wear process have been suspected to play an important role in this situation. Indeed, the peri-implant osteolysis could be due to the presence of particles stimulating the osteoclastogenesis process. We hypothesize then that the presence of a low particle concentration positively influences osteoblasts to produce osteoclastogenesis factors. If true, this hypothesis would then support the idea that the particles could be at the origin of the process leading to implant loosening. To check the validity of this hypothesis, we quantified in vitro the production of different genes involved in the osteoclastogenesis process using primary isolated human osteoblasts treated or not with particles. Results showed that low concentrations of particles might have a stimulating effect on osteoblasts to produce osteoclastogenesis factors as demonstrated by the increase of RANKL and CSF-1 gene expression in the particle group. r

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Production of osteocalcin by human bone cells in vitro. Effects of 1,25(OH)2D3, 24,25(OH)2D3, parathyroid hormone, and glucocorticoids

Cited by 494 publications

References 23 publications

Targeting subchondral bone for treating osteoarthritis: what is the evidence?

Targeting subchondral bone for treating osteoarthritis: what is the evidence?

Therapeutic touch affects DNA synthesis and mineralization of human osteoblasts in culture

The influence of wear particles in the expression of osteoclastogenesis factors by osteoblasts

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