Production of Ligninolytic Enzymes by Newly Isolated Bacteria from Palm Oil Plantation Soils

Abstract

Three aerobic lignin-degrading bacterial strains were isolated from palm oil plantation soils. The bacterial isolates were screened using a selective nutrient medium of minimum salt media (MSM), with kraft lignin as lignin substrate and methylene blue as the ligninolytic dye indicator. The newly isolated bacterial strains SHC1, SHC2, and SHC3 were found to have the potential to tolerate high concentrations of kraft lignin and produced all three main ligninolytic enzymes (lignin peroxidase, manganese peroxidase, and laccase); these strains may therefore be useful in the degradation of lignin in oil palm empty fruit bunch biomass. The production of ligninolytic enzymes was carried out by means of submerged fermentation for 7 days using 2 mm of oil palm empty fruit bunch (OPEFB) fiber as a substrate. These bacterial isolates were characterized using biochemical tests from Biolog and identified using 16S rRNA gene sequencing analysis, which identified the strains SHC1, SHC2, and SHC3 as Bacillus sp., Ochrobactrum sp., and Leucobacter sp., respectively with 99% sequence similarity. Bacillus sp. SHC1 produced the highest manganese peroxidase (MnP) of 2313.4 U/L on the third day and the highest lignin peroxidase (LiP) of 209.30 U/L on the fifth day of fermentation. The optimum pH and temperature for the production of ligninolytic enzymes by Bacillus sp. SHC1 were pH 8 and 30 °C.

A high throughput screening process and quick isolation of novel lignin-degrading microbes from large number of natural biomasses

“…Traditionally, lignin-degrading microorganisms have been selected based on qualitative methods such as direct screening using culture plates from environmental samples or by first enriching the environmental samples and then performing a plate-based screening [ 27 ]. The conventional screening process followed in previous studies is illustrated in the steps (left) shown in Fig.…”

A New Ciboria sp. for Soil Mycoremediation and the Bacterial Contribution to the Depletion of Total Petroleum Hydrocarbons

“…Leucobacter sp. has been described as capable to degrade hydrocarbons in polluted soil (Brzeszcz et al, 2020) and reported for its ligninolytic activity (Rahman et al, 2013), moreover involved in nitrogen cycle in soil (Solanki et al, 2017).…”

Bacillus aryabhattai: A Multi Metal Resistant Sewage Water Bacteria and Bioremediatory Tool for Sewage Water Pollutants

“…Later, this ingested compound is internalized and eventually degraded by different enzymes. Bacteria-derived ligninolytic laccase enzymes catalyze the mono-electronic oxidation of substrates and degrade a wide spectrum of pollutants from factories (Rahman et al, 2013). Till date, various bacterial strains have been isolated showing varying capabilities for the dissolution of varied pollutants or heavy metals; thus, it is necessary to identify a single bacterial strain capable of reducing the majority of pollutants as well as heavy metals.…”