Production of homocytotropic antibodies (IgE) to malathion in the rat

Vijay, H.M.; Mendoza, Celso E.; Lavergne, G.

doi:10.1016/0041-008x(78)90292-2

Cited by 5 publications

(3 citation statements)

References 5 publications

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“…However, heating of the reaginic sera (OA, DWSR and DWST) at 56 °C for 4 h or coprecipitation of rat antisera with a rabbit antiserum specific for rat myeloma IgE (kindly supplied by Dr. A. Froese, Uni versity of Manitoba, Winnipeg, Manitoba) according to the method of Conrad and Froese [3] completely abolished the reaginic activity of 2-, 4-and 48-hour PCA reac tions. These results support those of our earlier studies [16,17] and other investiga tors [4,12] who reported that it is extremely difficult to obtain an IgGa response with al um-precipitated antigens. However, Murphey et al [9] observed both IgE (a 48-hour PCA titer prior to heating of 128) as well as IgGa (a 2-hour PCA titer after heating at 56 °C for 1 h of 128) antibodies against OA in outbred Wistar rats using essentially the same method we described here.…”

supporting

confidence: 83%

Preferential Synthesis of IgE Reaginic Antibodies in Rats Immunized with Alum-Adsorbed Antigens

Vijay¹,

Lavergne²,

Huang³

et al. 1979

Int Arch Allergy Immunol

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The reaginic antibody response to alum-precipitated ovalbumin (OA) and the dialyzed water-soluble extracts of ragweed (DWSR) and Alternaria tenuis (DWST) in several strains of rats appeared to be wholly an IgE response. There was no evidence of a heat-stable (IgGa) antibody to OA, DWSR and DWST in the sera of the rats immunized with these antigens suspended in alum. Wistar-Furth and Lew inbred and hooded outbred rats produced comparable amounts of reaginic antibody after immunization with DWST, but BN inbred rats failed to generate a reaginic response to this antigen. The amount of antigen-induced histamine release from rat peritoneal mast cells did not always correlate with the level of circulating IgE-specific antibody.

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supporting

confidence: 83%

Preferential Synthesis of IgE Reaginic Antibodies in Rats Immunized with Alum-Adsorbed Antigens

Vijay¹,

Lavergne²,

Huang³

et al. 1979

Int Arch Allergy Immunol

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“…Repeated exposure to malathion results in allergic responses in man, guinea pigs, rabbits, rats, and mice (Centeno et al, 1970;Cushman and Street, 1983;Hazelton, 1992;Magnusson and Kligman, 1987;Milby and Epstein, 1964;Vijay et al, 1978). Repeated exposure to malathion results in allergic responses in man, guinea pigs, rabbits, rats, and mice (Centeno et al, 1970;Cushman and Street, 1983;Hazelton, 1992;Magnusson and Kligman, 1987;Milby and Epstein, 1964;Vijay et al, 1978).…”

Section: Malathionmentioning

confidence: 99%

Immunotoxicity of Pesticides

Rodgers

2001

Handbook of Pesticide Toxicology

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“…La primera aplicación de los métodos inmunoquímicos hacia los plaguicidas no fue sino hasta 1970 cuando Centeno y Johnson desarrollaron anticuerpos selectivamente para el malatión. 68 Años más tarde se desarrollaron radioinmunoensayos para aldrin y dieldrin, y para paration. [69][70] Posteriormente en 1972, Engvall y Perlman introdujeron el uso de enzimas como etiquetas para inmunoensayo.…”

Section: Incidenciaunclassified

Desarrollo de métodos inmunoquímicos para la determinación de sustancias tóxicas en alimentos y aguas

Cedeño¹,

Eudoro²

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The aim of this doctoral thesis is the study, development and validation of different immunochemical methods for determining chemical contaminants in produce and water, in a way that they may contribute to improving food quality and therefore to assure consumer safety. Spirotetramat is a highly efficient new-generation pesticide, marketed worldwide for use as insecticide in many agricultural crops. It has systemic properties, since short after absorption it translocates through both the xylem and the phloem, thanks to the fact that it is transformed by the plant into the much more polar spirotetramat-enol. Consequently, the residue definition for this insecticide in foods of plant origin with analytical purposes also includes said metabolite. On the other hand, anatoxin-a is a secondary alkaloid with acute neurotoxicity that can be found in fresh water. This toxin is produced by seven different genera of cyanobacteria, and has been detected in lakes and other water resources on all continents. The analysis of substances like spirotetramat and anatoxin-a is currently carried out by chromatographic methods like HPLC-MS. These techniques are highly sensitive and reliable; however, they require highly qualified personnel and expensive, non-portable equipment. Nowadays, the immunochemical methods, such as the ELISA (Enzyme-Linked ImmunoSorbent Assay) or the LFIA (Lateral Flow ImmunoAssay), constitute excellent complementary analytical options to instrumental strategies, since they are fast and inexpensive analytical methods, and are also very versatile so they can be adapted to particular analytical needs, such as screening assays for large numbers of samples or portable tests with visual reading of the results. The antibody affinity and specificity from a collection of spirotetramat and anatoxin-a immunoreagents was characterized in order to select conjugate/antibody pairs suitable for the development of competitive ELISA and LFIA tests. The assay conditions were optimized, and a study of the influence of different physicochemical factors over the analytical parameters of the selected immunoassays was carried out. Subsequently, the influence of the food matrix, particularly grape, grape juice and wine, as well as water from different sources, over the assay signal and sensitivity was evaluated. The different affinity of the available antibodies towards spirotetramat and spirotetramatenol led us to optimize the sample treatment procedure, so a hydrolysis step to transform spirotetramat into spirotetramat-enol in a controlled, rapid and quantitative way, was included. Thus, it was possible to provide results in the form of the sum of the concentration of both compounds in the sample, as required by current legislation. In addition, a procedure ~ 13 ~ using QuEChERS technology was developed to extract residues of this insecticide from grape samples, and polyvinylpolypyrrolidone was used to reduce interferences from wines and juices. In the case of waters, a simple filtration was applied to remove suspended particles. Mi...

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Production of homocytotropic antibodies (IgE) to malathion in the rat

Cited by 5 publications

References 5 publications

Preferential Synthesis of IgE Reaginic Antibodies in Rats Immunized with Alum-Adsorbed Antigens

Preferential Synthesis of IgE Reaginic Antibodies in Rats Immunized with Alum-Adsorbed Antigens

Immunotoxicity of Pesticides

Desarrollo de métodos inmunoquímicos para la determinación de sustancias tóxicas en alimentos y aguas

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