Production of high crystallinity type‐I cellulose from Komagataeibacter hansenii JR‐02 isolated from Kombucha tea

Abstract

In this study, a bacterial cellulose (BC) producing strain was isolated from Kombucha tea and identified as Komagataeibacter hansenii JR-02 by morphological, physiological, and biochemical characterization and 16S rRNA sequence. Then, the media components and culture conditions for BC production were optimized. Result showed that the highest BC yield was 3.14 ± 0.22 and 8.36 ± 0.19 g/L after fermentation for 7 days under shaking and static cultivation, respectively. Moreover, it was interesting that JR-02 could produce BC in nitrogen-free medium with the highest yield of 0.76 ± 0.06 g/L/7days, and the possible nitrogen fixation gene nifH was cloned from its genomic DNA.The BC produced by JR-02 was type-I cellulose with high crystallinity and thermodynamic stability, which was revealed from Fourier transform infrared spectroscopy, X-ray diffraction, and thermogravimetric analysis methods. The crystallinity of static and shaking cultured BC were 91.76% and 90.69%, respectively. The maximum rate of weight loss of static and shaking BC occurred at temperature of approximately 373.1 • C and 369.1 • C, respectively. Overall, these results indicated that K. hansenii JR-02 had great potential to produce high crystallinity type-I BC in manufacture.Abbreviations: β, β is the line broadening at half the maximum intensity (FWHM) in radians; λ, λ is the X-ray wavelength (1.54Å); θ , θ is the Bragg's angle; +, the experiment result is positive; −, the experiment result is negative; (+), the experiment result is weak positive; BC, bacterial cellulose; Cel+, BC producing strains; Cel−, BC producing strains lost the function of bacterial cellulose producing; CrI, crystallinity index; CrS, crystallite size; CSL, corn steep liquor; d-spacing, interplanar crystal distance; DTG, differential thermal analysis; FE-SEM, Field emission scanning electron microscopy; FTIR, fourier transform infrared; Iam, the minimum intensity between (101) and (002) peaks; I002, I002 is the intensity at (002) peak which is showed in the XRD profiles of BC; k, k is the shape factor (0.9); nr, The experiment result is not reported; SK, BC obtained from shaking culture; ST, BC obtained from static culture; TCA, tricarboxylic acid cycle; TGA, thermogravimetric analysis; UDPG, uridine 5 -diphosphate glucose; XRD, X-ray diffractometry; YE, yeast extract. .

Effect of water and alkali on purification bacterial cellulose membrane from Kombucha

“…The bands at 1145, 1151, and 1106 cm -1 , indicate the presence of the NH group. According to Li et al, (2019) and collaborators the peaks representing proteins are 1062.6-1059.1 cm -1 ; however, in this work, these peaks for the membrane proteins were 926, 1010, and 1016cm -1 . The results of the spectra of pure KBCM, KBCM purified with water, and KBCM purified with NaOH are shown in Figure 5.…”

Bacterial Cellulose: Production, Modification and Perspectives in Biomedical Applications

“…The species has been for many years known as Acetobacter xylinum , but has been later classified into Gluconacetobacter xylinus and due to further taxonomic changes finally reclassified into Komagataeibacter xylinus . K. xylinus is not the only species among AAB with an immense potential for BC production, since also other species, such as Komagataeibacter hansenii , Komagataeibacter medellinensis , Komagataeibacter nataicola , Komagataeibacter oboediens , Komagataeibacter rhaeticus , Komagataeibacter saccharivorans and Komagataeibacter pomaceti have been characterized as strong cellulose producers [4,6,7,8,9]. An important aspect of using AAB for cellulose production is their characteristic of being food-grade or GRAS bacteria (generally recognized as safe).…”

The Space-Exposed Kombucha Microbial Community Member Komagataeibacter oboediens Showed Only Minor Changes in Its Genome After Reactivation on Earth

“…Gluconobacter kombuchae (now K. hansenii ) isolated from Kombucha tea has been shown to have nitrogen-fixing capabilities ( Dutta and Gachhui, 2007 ). Nevertheless, putative nitrogen fixation/regulation genes such as nif H in K. hansenii JR-02 ( Li et al, 2019 ) and nifU, ntrB, ntrC , ntrX , and ntrY in BC producer K. rhaeticus ENS9a are reported, which are not homologous to nif HDK of Klebsiella oxytoca or other nitrogen-fixing bacteria. In K. oboediens IMBG180, no structural genes of nitrogenase were found, and, to date, no experimental evidence of N-fixation by these species was reported.…”