Production of cutinase by solid-state fermentation and its use as adjuvant in bioherbicide formulation

Oliveira, Caroline Torres de; Alves, Eliana A.; Todero, Izelmar; Kuhn, Raquel C.; Oliveira, Débora de; Mazutti, Márcio A.

doi:10.1007/s00449-019-02086-w

Cited by 12 publications

(3 citation statements)

References 39 publications

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“…The success of the application of phytotoxic fungal metabolites requires obtaining a formulation with a high weed control potential and with desired properties, such as stability, appropriate chemical characteristics allowing its storage and sufficient shelf‐life of the product, as well as its long‐term use. The formulation should also facilitate uptake of the active compound by the target weed 91 . One challenge in the development of natural herbicides is their low herbicidal activity.…”

Section: Methodological Approaches Used To Discover Identify and Expl...mentioning

confidence: 99%

“…The formulation should also facilitate uptake of the active compound by the target weed. 91 One challenge in the development of natural herbicides is their low herbicidal activity. In general, the low effect occurs because the fungal phytotoxins are found in very low concentration in culture media.…”

Section: Exploitation Of Metabolites Of Interest: Towards the Formula...mentioning

confidence: 99%

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Phytotoxic fungal secondary metabolites as herbicides

Bendejacq‐Seychelles,

Gibot‐Leclerc,

Guillemin

et al. 2023

Pest Management Science

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Among the alternatives to synthetic plant protection products, biocontrol appears as a promising method. This review reports on the diversity of fungal secondary metabolites phytotoxic to weeds and on the approach generally used to extract, characterize, identify and exploit them for weed management. The 183 phytotoxic fungal secondary metabolites discussed in this review fall into five main classes of molecules: 61 polyketides, 53 terpenoids, 36 nitrogenous metabolites, 18 phenols and phenolic acids, and 15 miscellaneous. They are mainly produced by the genera Drechslera, Fusarium and Alternaria. The phytotoxic effects, more often described by the symptoms they produce on plants than by their mode of action, range from inhibition of germination to inhibition of root and vegetative growth, including tissue and organ alterations. The biochemical characterization of fungal secondary metabolites requires expertise and tools to carry out fungal cultivation and metabolite extraction, phytotoxicity tests, purification and fractionation of the extracts, and chemical identification procedures. Phytotoxicity tests are mainly carried out under controlled laboratory conditions (not always on whole plants), while effectiveness against targeted weeds and environmental impacts must be assessed in greenhouses and open fields. These steps are necessary for the formulation of effective, environment‐friendly fungal secondary metabolites‐derived bioherbicides using new technologies such as nanomaterials.This article is protected by copyright. All rights reserved.

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Section: Methodological Approaches Used To Discover Identify and Expl...mentioning

confidence: 99%

Section: Exploitation Of Metabolites Of Interest: Towards the Formula...mentioning

confidence: 99%

Phytotoxic fungal secondary metabolites as herbicides

Bendejacq‐Seychelles,

Gibot‐Leclerc,

Guillemin

et al. 2023

Pest Management Science

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“…Plant cutin acts as an important inducer of cutinase enzyme for different fungal species. Several studies have reported that Fusarium species are able to produce cutinase (Kim et al, 2007;Fraga et al, 2012;Canavati-Alatorre et al, 2017;De Oliveira et al, 2019). In this research, different concentrations of apple peels cutin were evaluated for growth and cutinase production by Fusarium culmorum in submerged fermentation.…”

Section: Introductionmentioning

confidence: 96%

Production of cutinolytic esterase by Fusarium culmorum grown at different apple cutin concentrations in submerged fermentation

et al. 2019

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Cutinolytic esterase (i.e., cutinase) is an enzyme that catalyzes the cleavage of ester bonds in cutin and also in diverse soluble and insoluble esters. It has application in several biotechnological areas, acting as biocatalysts in the food industry, in detergents, in biodegradation of polymers and other toxic substances, being important in biorremediation. In this research, specific growth rate, protein content, cutinolytic activity by biochemical tests and polyacrylamide gel electrophoresis, and growth and enzymatic kinetic parameters were determined for F. culmorum grown at different apple cutin concentrations (0.2, 2 and 20 g/L) in submerged fermentation. It was observed that biomass, protein content and enzymatic activity enhanced as cutin concentration increased in the media. A cutinase activity band of around 65 KDa was observed in zymograms of different cutin concentration. An additional cutinase activity band of around 90 KDa was also observed in zymograms of F. culmorum grown in 20 g of apple cutin/L. These studies showed that F. culmorum used apple cutin as the sole carbon source, which acted as a cutinase inducer. The highest-yielding parameters of cutinase were observed in 2 g of apple cutin/L. This research showed promising results in the cutinase induction for F. culmorum using a low concentration of apple cutin.

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