Production of conidial biomass of Trichoderma harzianum for biological control

Harman, Gary E.; Jin, Xixuan; Stasz, T. E.; Peruzzotti, G.; Leopold, A. C.; Taylor, A.G.

doi:10.1016/1049-9644(91)90097-j

Cited by 107 publications

(65 citation statements)

References 16 publications

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“…, 1994). Intracellular accumulation of trehalose has been associated with prolonged storage life of conidia of Tricboderma harqjantlm (Harman e t al., 1991), Aspergilltlsjaponiczis (Gornova e t al., 1992), B. bassiana, M. anisopliae and P.farin0stl.r (Hallsworth & Magan, 1994~). Conidia of entomopathogens can contain > 200 mg trehalose 8-l when cultures are supplied with a high concentration of trehalose (Hallsworth & Magan, 1994b).…”

Section: Introductionmentioning

confidence: 99%

Manipulation of intracellular glycerol and erythritol enhances germination of conidia at low water availability

Hallsworth¹,

1995

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The insect pathogens Beauveria bassiana, Metarhizium anisopliae and Paecilomyces farinosus can be effective biocontrol agents when relative humidity (RH) is close to 100%. At reduced water availability, germination of propagules, and therefore host infection, cannot occur. Cultures of B. bassiana, M. anisopliae and P. farinosus were grown under different conditions t o obtain conidia with a modified polyol and trehalose content. Conidia with higher intracellular concentrations of glycerol and erythritol germinated both more quickly and at lower water activity (aw) than those from other treatments. In contrast, conidia containing up t o 2357 mg trehalose g-' germinated significantly (P < 095) more slowly than those with an equivalent polyol content but less trehalose, regardless of water availability. Conidia from control treatments did not germinate below 0*951-0.935 a , ( = 951-935 O/ O RH).In contrast, conidia containing up to 1646 mg glycerol plus erythritol g-1 germinated down to 0.887 a , (= 887?40 RH). These conidia germinated below the water availability at which mycelial growth ceases (093010.920 a , ) . Germ tube extension rates reflected the percentage germination of conidia, so the most rapid germ tube growth occurred after treatments which produced conidia containing the most glycerol and erythritol. This study shows for the first time that manipulating polyol content can extend the range of water availability over which fungal propagules can germinate. Physiological manipulation of conidia may improve biological control of insect pests in the field.

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Section: Introductionmentioning

confidence: 99%

Manipulation of intracellular glycerol and erythritol enhances germination of conidia at low water availability

Hallsworth¹,

1995

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“…Aerial spores produced on solid media are unsuitable for large scale production because it is difficult to maintain the cultures under the optimal temperature and airflow conditions. 18,19) Liquid fermentation has been generally employed to produce submerged spores from this fungus. [19][20][21] Most current research on the practical implementation of BCAs focuses on the optimum production of spores able to guarantee stability and economy.…”

Section: Introductionmentioning

confidence: 99%

“…18,19) Liquid fermentation has been generally employed to produce submerged spores from this fungus. [19][20][21] Most current research on the practical implementation of BCAs focuses on the optimum production of spores able to guarantee stability and economy. [20][21][22][23][24][25] In previous studies, we reported the biological control activity of T. asperellum SKT-1 using aerial spores (i.e.…”

Section: Introductionmentioning

confidence: 99%

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Properties and biological control activities of aerial and submerged spores in Trichoderma asperellum SKT-1

Watanabe

Kato²,

Kumakura

et al. 2006

J. Pestic. Sci.

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Trichoderma asperellum SKT-1, a biocontrol agent that is highly effective against rice seedborne diseases, forms submerged spores by liquid culture. These submerged spores had some common properties with aerial spores, but differed in surface topology and internal organization. Aerial spores, so called conidia, maintained high viability under dry storage conditions, whereas the viability of submerged spores was reduced. The cell wall of submerged spores was thinner than that of aerial spores, and the surface was smooth in contrast to the irregular pyramidal warts of aerial spores. Both submerged and aerial spores were highly effective against "Bakanae" disease by seed-treatment. Therefore, these two-types of spores were suitable for different applications.

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“…The quality of a microbial bio-protectant is dependent on the propagate density in the biomass and its ability to survive processing (Harman et al, 1991). Production of adequate quantities of good quality inoculum is an essential component of the biocontrol programme.…”

Section: Introductionmentioning

confidence: 99%

Agro-based Waste Products as a Substrate for Mass Production of Trichoderma spp.

Simon¹,

Anamika²

2011

JAS

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Various agricultural by products such as vegetable wastes, fruit wastes, crop wastes, FYM and Poultry manure were evaluated for mass production of Trichoderma viride and Trichoderma harzianum. Among the substrates used wastes of brinjal, banana, papaya, guava, spinach, sugarcane, tealeaves and pea husk of solid and liquid was found supported maximum spore production insolid and liquid for the growth of Trichoderma harzianum and Trichoderma viride as compared with wastes of carrot, cucumber, potato, radish, cabbage, orange peel, wheat bran, FYM and Poultry manure.

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Production of conidial biomass of Trichoderma harzianum for biological control

Cited by 107 publications

References 16 publications

Manipulation of intracellular glycerol and erythritol enhances germination of conidia at low water availability

Manipulation of intracellular glycerol and erythritol enhances germination of conidia at low water availability

Properties and biological control activities of aerial and submerged spores in Trichoderma asperellum SKT-1

Agro-based Waste Products as a Substrate for Mass Production of Trichoderma spp.

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