Production of chiral compounds using immobilized cells as a source of biocatalysts

Kisukuri, Camila M.; Andrade, Leandro H.

doi:10.1039/c5ob01677k

Cited by 40 publications

(22 citation statements)

References 92 publications

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“…To minimize the non‐stereoselective background reaction at elevated substrate concentrations, we anticipated that this may be achieved using immobilized enzyme in flow. Advantages and methods of enzyme immobilization are reviewed in recent articles . Immobilized enzymes have been reported to display improved stability, reusability, enable simplified product purification, and therefore more efficient processes compared to the reaction with free enzymes.…”

Section: Resultsmentioning

confidence: 99%

“…Advantages and methods of enzyme immobilization are reviewed in recent articles. [33][34][35][36][37][38][39][40][41] Immobilized enzymes have been reported to display improved stability, reusability, enable simplified product purification, and therefore more efficient processes compared to the reaction with free enzymes.…”

Section: Immobilization For Batch Reactions As Well As In Flowmentioning

confidence: 99%

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Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations

Lechner

Soriano

Poschner

et al. 2017

Biotechnology Journal

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Norcoclaurine synthases (NCS), catalyzing a Pictet-Spengler reaction in plants as one of the first enzymes in the biosynthetic benzylisoquinoline pathway, are investigated for biocatalytic transformations. The library of NCS available is extended by two novel NCSs from Argemone mexicana (AmNCS1, AmNCS2) and one new NCS from Corydalis saxicola (CsNCS); furthermore, it is shown that the NCS from Papaver bracteatum (PbNCS) is a highly productive catalyst leading to the isoquinoline product with up to >99% e.e. Under certain conditions lyophilized whole Escherichia coli cells containing the various overexpressed NCS turned out to be suitable catalysts. The reaction using dopamine as substrate bears several challenges such as the spontaneous nonstereoselective background reaction and side reactions. The PbNCS enzyme is successfully immobilized on various carriers whereby EziG3 proved to be the best suited for biotransformations. Dopamine showed limited stability in solution resulting in the coating of the catalyst over time, which could be solved by the addition of ascorbic acid (e.g., 1 mg ml À1 ) as antioxidant.

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Section: Resultsmentioning

confidence: 99%

Section: Immobilization For Batch Reactions As Well As In Flowmentioning

confidence: 99%

Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations

Lechner

Soriano

Poschner

et al. 2017

Biotechnology Journal

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“…For example, the whole-cell biocatalyst system has been enhanced to immobilize the enzymes and improve substrate-enzyme contact, while increasing the catalytic potential of the enzymes by extending their overall lifetime (Kisukuri and Andrade 2015).…”

Section: Gh Whole-cell Systemsmentioning

confidence: 99%

Lignocellulases: a review of emerging and developing enzymes, systems, and practices

Obeng

Adam

Budiman

et al. 2017

Bioresour. Bioprocess.

127

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The highly acclaimed prospect of renewable lignocellulosic biocommodities as obvious replacement of their fossilbased counterparts is burgeoning within the last few years. However, the use of the abundant lignocellulosic biomass provided by nature to produce value-added products, especially bioethanol, still faces significant challenges. One of the crucial challenging factors is in association with the expression levels, stability, and cost-effectiveness of the cellulose-degrading enzymes (cellulases). Interestingly, several recommendable endeavors in the bid to curb these challenges are in pursuance. However, the existing body of literature has not well provided the updated roadmap of the advancement and key players spearheading the current success. Moreover, the description of enzyme systems and emerging paradigms with high prospects, for example, the cell-surface display system has been ill-captured in the literature. This review focuses on the lignocellulosic biocommodity pathway, with emphasis on cellulase and hemicellulase systems. The paradigm shift towards cell-surface display system and its emerging recommendable developments have also been discussed. The attempts in supplementing cellulase with other enzymes, accessory proteins, and chemical additives have also been discussed. Moreover, some of the prominent and influential discoveries in the cellulase fraternity have been discussed.

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“…The selection of bioreceptors should be based on their ability to bind specific target materials and should consider their capacity for immobilization (Singh et al, 2013). Kisukuri and Andrade (2015).…”

Section: Biosensorsmentioning

confidence: 99%

Biosensors and their Applications in Food Safety: A Review

Yasmin¹,

Ahmed²,

Cho³

2016

Journal of Biosystems Engineering

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Background: Foodborne pathogens are a growing concern with respect to human illnesses and death. There is an increasing demand for improvements in global food safety. However, it is a challenge to detect and identify these harmful organisms in a rapid, responsive, suitable, and effective way. Results: Rapid developments in biosensor designs have contributed to the detection of foodborne pathogens and other microorganisms. Biosensors can automate this process and have the potential to enable fast analyses that are cost and time-effective. Various biosensor techniques are available that can identify foodborne pathogens and other health hazards. Conclusions: In this review, biosensor technology is briefly discussed, followed by a summary of foodborne pathogen detection using various transduction systems that exhibit specificity for particular foodborne pathogens. In addition, the recent application of biosensor technology to detect pesticides and heavy metals is briefly addressed.

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Production of chiral compounds using immobilized cells as a source of biocatalysts

Cited by 40 publications

References 92 publications

Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations

Library of Norcoclaurine Synthases and Their Immobilization for Biocatalytic Transformations

Lignocellulases: a review of emerging and developing enzymes, systems, and practices

Biosensors and their Applications in Food Safety: A Review

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