Production of bovine calves following separation of X- and Y-chromosome bearing sperm and in vitro fertilisation

Cran, D. G.; Johnson, Lawrence A.; Miller, Nigel; Cochrane, Diane; Polge, C.

doi:10.1136/vr.132.2.40

Cited by 148 publications

(87 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…laser impact have been expressed by some authors (Ashwood-Smith 1994, Munné 1994, especially after the successful application of this technology to the separation of human spermatozoa (Johnson et al 1993). It is well known that both Hoechst 33342 and u.v.…”

Section: Discussionmentioning

confidence: 99%

Hoechst 33342 stain and u.v. laser exposure do not induce genotoxic effects in flow-sorted boar spermatozoa

Parrilla¹,

Vázquez²,

Cuello³

et al. 2004

Reproduction

View full text Add to dashboard Cite

Sex selection by flow cytometry/cell sorting involves the staining of spermatozoa with Hoechst 33342 in combination with the impact of a u.v. laser beam, two potentially mutagenic agents. A phenotypic and cytogenetic study of lymphocytes of piglets born after insemination with spermatozoa stained with Hoechst 33342 and from piglets obtained from stain-sorted spermatozoa was performed to evaluate the genotoxic effect of Hoechst 33342 staining and u.v. laser irradiation on the offspring. Lymphocytes from piglets born after insemination with unstained spermatozoa, but from the same ejaculate, were used as a control group. Peripheral blood lymphocytes from these piglets were cultured following a standard cell culture protocol. Cells were then collected by centrifugation, subjected to hypotonic solution and fixed and dropped onto slides. Sister chromatid exchanges (SCEs) and chromosome aberrations (CAs: including chromosome and chromatid breaks) per cell were scored in 50-s division metaphase spreads from each donor. Reproductive parameters and litter performance of all inseminations performed were also recorded in all groups. Data were analyzed by ANOVA. No significant increase (P > 0.05) of SCE and CA frequencies were observed in piglets born from stained spermatozoa or from stain-sorted spermatozoa with respect to controls (untreated sperm). The results indicated that no mutagenic effect on spermatozoa, expressed as increases in the incidence of abnormalities in the resulting offspring and also as increases in SCE and CA frequencies on lymphocytes from these individuals, was induced by the staining of boar spermatozoa with Hoechst 33342, nor by combination of staining with laser impact during flow cytometry.

show abstract

Section: Discussionmentioning

confidence: 99%

Hoechst 33342 stain and u.v. laser exposure do not induce genotoxic effects in flow-sorted boar spermatozoa

Parrilla¹,

Vázquez²,

Cuello³

et al. 2004

Reproduction

View full text Add to dashboard Cite

show abstract

“…Therefore, it seems obvious to use sex-sorted spermatozoa in IVF. An early report on bovine IVF with sex-sorted spermatozoa, Cran et al (1993) reported birth of the first calves where the sex was determined before fertilisation. Cleavage rates were similar to unsorted controls, but blastocyst development was diminished after IVF with sexed spermatozoa, presumably due to capacitation-like reorganisation of the sperm membranes (Merton et al 1997, Lu et al 1999.…”

Section: Ivf With Sorted Spermatozoamentioning

confidence: 99%

Sex selection of sperm in farm animals: status report and developmental prospects

Rath¹,

Barcikowski²,

Graaf³

et al. 2013

Reproduction

View full text Add to dashboard Cite

Pre-selection of spermatozoa based on the relative DNA difference between X-and Y-chromosome bearing populations by flow cytometry is an established method that has been introduced into commercial cattle production. Although several important improvements have increased the sort efficiency, the fertilising ability of sexed spermatozoa based on offspring per insemination is still behind farmers' expectations. The main stress factors, especially on mitochondria, that reduce the lifespan of spermatozoa are described, and new technical as well as biological solutions to maintain the natural sperm integrity and to increase the sorting efficiency are discussed. Among these methods are the identification of Y-chromosome bearing spermatozoa by bi-functionalised gold nanoparticles and triplex hybridisation in vivo as well as new laser-controlled deflection system that replaces the deflection of spermatozoa in the electrostatic field. Additionally, as well as a new nonsurgical transfer system of spermatozoa into the oviduct of cows has been developed and allows a significant reduction of spermatozoa per transfer. Altogether, the improvements made in the recent years will allow a broader use of sex-sorted spermatozoa even in those species that require more cells than cows and sheep.Reproduction (2013) 145 R15-R30

show abstract

“…La Fertilización in vitro (FIV) se ha usado en la industria ganadera hace ya algunas décadas, el primer ternero vivo, obtenido por FIV, nació en 1981 (Cran et al, 1993). Los primeros terneros nacidos por FIV con SX, nacieron en 1993 (Cran et al, 1993).…”

Section: Introductionunclassified

“…Los primeros terneros nacidos por FIV con SX, nacieron en 1993 (Cran et al, 1993). Se han hecho desde entonces estudios en diferentes partes del mundo y en diferentes especies que han contribuido a la efectividad de la técnica (Zhang et al, 2003;Lu & Seidel Jr., 2004;Xu et al, 2006;Trigal et al, 2012).…”

Section: Introductionunclassified

Semen Bovino Sexado Congelado-Descongelado en Producción de Embriones In vitro

Larocca

Filipiak

2017

Int. J. Morphol.

View full text Add to dashboard Cite

Production of bovine calves following separation of X- and Y-chromosome bearing sperm and in vitro fertilisation

Cited by 148 publications

References 0 publications

Hoechst 33342 stain and u.v. laser exposure do not induce genotoxic effects in flow-sorted boar spermatozoa

Hoechst 33342 stain and u.v. laser exposure do not induce genotoxic effects in flow-sorted boar spermatozoa

Sex selection of sperm in farm animals: status report and developmental prospects

Semen Bovino Sexado Congelado-Descongelado en Producción de Embriones In vitro

Contact Info

Product

Resources

About