Production of Bacillus sphaericus larvicide on industrial peptones

Klein, D.; Yanai, Peter; Hofstein, R.; Fridlender, Bertold; Braun, Sergei

doi:10.1007/bf00255363

Cited by 13 publications

(11 citation statements)

References 11 publications

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“…Addition of corn steep liqure at 0.2-0.5% showed no effect on toxicity, however, increasing its concentration to 1% has a dramatic effect on mosquitocidal activity. It is known that whey permeate contains amino acids mainly glutamic acid, aspartic acid, proline and minerals (El-Bendary et al 2008) which promote the growth and toxin production of B. sphaericus (Klein et al 1989;Lacey 1984;Shevtsov et al 1990). …”

Section: Effect Of Additivesmentioning

confidence: 98%

Production of mosquitocidal Bacillus sphaericus by solid state fermentation using agricultural wastes

El-Bendary

2009

World J Microbiol Biotechnol

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In this study, Bacillus sphaericus NRC 69 was grown in culture media, in which 12 agricultural wastes were tested as the main carbon, nitrogen and energy sources under solid state fermentation. Of the 12 tested agricultural by-products, wheat bran was the most efficient substrate for the production of B. sphaericus mosquitocidal toxins against larvae of Culex pipiens (LC 50 1.2 ppm). Mixtures of tested agricultural wastes separately with wheat bran enhanced the produced toxicity several folds and decreased LC 50 between 3.7-and 50-fold in comparison with that of agricultural wastes without mixing. The toxicity of B. sphaericus grown in wheat bran/rice hull at 8/2 (g/g) and wheat bran/barley straw at 1/4 (g/g) showed the same toxicity as that in wheat bran medium (LC 50 decreased 17-and 16-fold, in comparison with that in rice hull or barely straw media, respectively). In wheat bran medium, the maximum toxicity of the tested organism obtained at 50% moisture content, inoculum size 84 9 10 6 CFU/g wheat bran and incubation for 6 days at 30°C. Addition of cheese whey permeate at 10% to wheat bran medium enhanced the toxicity of B. sphaericus NRC 69 about 46%.

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Section: Effect Of Additivesmentioning

confidence: 98%

Production of mosquitocidal Bacillus sphaericus by solid state fermentation using agricultural wastes

El-Bendary

2009

World J Microbiol Biotechnol

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“…Instead, it can use amino acids as carbon and nitrogen sources. The amino acid analysis of WP showed that the most abundant amino acids were glutamic acid (0.296 mg/ml) and aspartic acid (0.153 mg/ml) (Table 1) which serve as the primary carbon and nitrogen sources for growth and toxin production of B. sphaericus in proteinaceous media (Klein et al, 1989;Lacey, 1984;Yousten et al, 1984).…”

Section: Effect Of Supplementation Of Wp With Nitrogen Sourcesmentioning

confidence: 99%

“…Various agro-industrial by-products and animal byproducts have been used as raw materials for mosquitocidal toxin production by B. sphaericus under submerged fermentation such as cow blood, industrial peptones, corn steep liquor, ground nut cake, cotton seed meal, offals meal, soybean meal, fodder yeast, mustard seed meal and brewers' spent grain (Ampofo, 1995;Desai and Shethna, 1991;El-Bendary, 1999;El-Bendary, 2006;Gangurde and Shethna, 1995;Klein et al, 1989;Kuppusamy and Balaraman, 1991;Obeta and Okafor, 1983;Sasaki et al, 1998).…”

Section: Introductionmentioning

confidence: 98%

Efficient mosquitocidal toxin production by Bacillus sphaericus using cheese whey permeate under both submerged and solid state fermentations

El-Bendary

Moharam

Foda

2008

Journal of Invertebrate Pathology

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“…Indeed, glucose, lactose, sucrose, fructose, galactose, arabinose, maltose and xylose at concentrations 10-2-10-8 M were not attractants at any stage of culture growth. Although glycerol was used as a carbon source [15], it failed to attract B. sphaericus cells. Attempts to induce chemotaxis to sugars or glycerol by including these compounds in the growth medium, or by incubating cells for one hour in the sugar-supplemented chemotaxis buffer followed by removal of the sugar, or both treatments, failed to induce chemotaxis.…”

Section: Methodsmentioning

confidence: 99%

“…At the early logarithmic stage, alanine was the most potent attractant, as it is for B. subtilis [14]. Lysine, glutamate, and glycine while good substrates [15] acted as poorer attractants.…”

Section: Methodsmentioning

confidence: 99%

Motility and chemotaxis in Bacillus sphaericus dependence upon stage of growth

1994

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Chemotaxis and motility of Bacillus sphaericus 2362 were monitored as a function of the batch culture age. It was found that both functions changed independently during growth of the culture. Motility was low until the late logarithmic stage ensued, whereafter it increased sharply. The ability of cells to respond to chemo-effectors peaked at the mid-logarithmic phase. A major methyl-accepting chemotaxis protein (P53, M, = 53 kDa) was identified. The extent of label incorporation in this protein from L-[methyl-'Hlmethionine was maximal in mid-and late-logarithmic phases of the growth. Cells in stationary cultures incorporated very low amounts of the label. At any stage, the labeling was maximal in starved cells; it was almost abolished in cells pre-incubated with amino acids. Although extents of P53 labeling in mid-and late logarithmic cells were similar, late logarithmic cells demonstrated a considerably impaired chemotaxis. Supermotile sporulating cells were practically insensitive to environmental stimuli. The dflerence in development of sensory and locomotive functions may be interpreted as an adaptive response. A well developed sensory apparatus would allow vegetative cells to adapt efficiently to fluctuating attractant gradients. Insensitive sporulating cells would tend to disperse randomly from the nutrient-exhausted area. Thus, spore formation would occur in larger volume of the habitat, increasing the chance of microbial population to survive.

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Production of Bacillus sphaericus larvicide on industrial peptones

Cited by 13 publications

References 11 publications

Production of mosquitocidal Bacillus sphaericus by solid state fermentation using agricultural wastes

Production of mosquitocidal Bacillus sphaericus by solid state fermentation using agricultural wastes

Efficient mosquitocidal toxin production by Bacillus sphaericus using cheese whey permeate under both submerged and solid state fermentations

Motility and chemotaxis in Bacillus sphaericus dependence upon stage of growth

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