Production of a generic microbial feedstock for lignocellulose biorefineries through sequential bioprocessing

Chang, Chen-Wei; Webb, Colin

doi:10.1016/j.biortech.2016.12.055

Cited by 15 publications

(7 citation statements)

References 35 publications

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“…Other authors have even employed mixtures of residues, such as sugarcane bagasse and soybean hull or food and bakery waste. Afterward, fermented solids were used as a source of enzymes to obtain fermentation feedstocks [ 11 , 12 , 13 , 14 , 15 ].…”

Section: Introductionmentioning

confidence: 99%

Conversion of Exhausted Sugar Beet Pulp into Fermentable Sugars from a Biorefinery Approach

Marzo

Díaz

Caro

et al. 2020

Foods

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In this study, the production of a hydrolysate rich in fermentable sugars, which could be used as a generic microbial culture medium, was carried out by using exhausted sugar beet pulp pellets (ESBPPs) as raw material. For this purpose, the hydrolysis was performed through the direct addition of the fermented ESBPPs obtained by fungal solid-state fermentation (SSF) as an enzyme source. By directly using this fermented solid, the stages for enzyme extraction and purification were avoided. The effects of temperature, fermented to fresh solid ratio, supplementation of fermented ESBPP with commercial cellulase, and the use of high-solid fed-batch enzymatic hydrolysis were studied to obtain the maximum reducing sugar (RS) concentration and productivity. The highest RS concentration and productivity, 127.3 g·L−1 and 24.3 g·L−1·h−1 respectively, were obtained at 50 °C and with an initial supplementation of 2.17 U of Celluclast® per gram of dried solid in fed-batch mode. This process was carried out with a liquid to solid ratio of 4.3 mL·g−1 solid, by adding 15 g of fermented solid and 13.75 g of fresh solid at the beginning of the hydrolysis, and then the same amount of fresh solid 3 times every 2.5 h. By this procedure, ESBPP can be used to produce a generic microbial feedstock, which contains a high concentration of monosaccharides.

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Section: Introductionmentioning

confidence: 99%

Conversion of Exhausted Sugar Beet Pulp into Fermentable Sugars from a Biorefinery Approach

Marzo

Díaz

Caro

et al. 2020

Foods

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“…In the solid substrates fermentation, water content is one of the important biological factors for fungi growth (Chang & Webb 2017). Moisture content influenced the growth of mold and production of BS4 enzymes (mannanase and saccharification activities).…”

Section: Moisture Content In the Course Of Fermentationmentioning

confidence: 99%

“…The DML values are also related to the water content of the fermented product. In solid substrate fermentation, the moisture content is one of the important biological factors for mold growth (Chang & Webb 2017). In general, the water content of solid substrate fermentation ranges from 30 to 85% (Raimbault 1998).…”

Section: Dry Matter Losses (Dml) In the Course Of Fermentationmentioning

confidence: 99%

Optimization of BS4 Enzyme production with Different Substrate Thickness and Type of Trays

Haryati¹,

Sinurat²,

Hamid³

et al. 2019

JITV

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BS4 enzyme that is produced from solid substrate fermentation (SSF) on coconut cake with Eupenicillium javanicum BS4 in tray bioreactor has been applied as a feed additive. It increases the nutritional value of animal feedstuff. The BS4 production on SSF may be influenced by the better aeration through the perforated trays or by the thinner substrate. The aim of this research is to optimize the production of BS4 with different substrate thicknesses and types of trays. The trial was carried out using a factorial randomized design (2x2x3) with 6 replicates. The first factor was the type of trays: i.e., non-perforated and perforated tray. The second factor was the thickness of the substrate: i.e., 1.5 and 3.0 cm, while the third factor was the duration of fermentation: i.e. 5, and 7 days. The variables observed were moisture content, dry matter loss (DML), mannanase and saccharification activities, soluble protein content, their specific activities, and yield. Statistical analysis showed no interactions between the three factors, but there were interactions between types of trays and substrate thicknesses, as well as type of trays and incubation times on the mannanase activity and yield of mannanase. The results showed that DML was observed on day 7 were around 31.43- 36.89. The highest mannanase activity was observed on the non-perforated tray with 3 cm thickness on day 7. The saccharification activity towards palm kernel meal was better in the non-perforated tray on day 7 but not influenced by The yield value of mannanase and saccharification activities on a non-perforated tray with 3.0 cm thickness on day 7 was also the highest. Based on energy efficiency and the cost of production, it can be concluded that the optimum condition to produce the BS4 enzyme was observed in the non-perforated tray with 3 cm thickness and fermented for 7 days.

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“…The microalgae Spirulina [10,23], Chlorella [10,43], Chlorella protothecoides [51], Microcystis aeruginosa, Nannochloropsis sp. [43,44,52,53], Chlorella pyrenoidosa, Bacillariophyta sp., Scenedesmus dimorphous [11], Porphyridium [28], and Butyroccoccus or a mixture of strains are promising feedstock with high production efficiency, fast growth rate, and round the year production and cultivation in non-arable lands [34,36,40,50,[54][55][56]. A variety of microalgae feedstock was obtained from natural resources such as lake and open water, especially when microalgae blooms, or from artificial cultivation systems [57].…”

Section: Microalgae Characteristics Pretreatment and Preparationmentioning

confidence: 99%

Continuous Hydrothermal Liquefaction for Biofuel and Biocrude Production from Microalgal Feedstock

Lababpour¹

2018

ChemBioEng Reviews

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Microalgal biomass processing by continuous hydrothermal liquefaction (HTL) has recently received much interest and can be used to convert microalgal biomass to biocrude oil and drop‐in fuels. This review focuses on the conversion of microalgae biomass to energy production through continuous HTL processes. In addition, processes for upgrading feedstocks and biocrude are also discussed. The feed of the biomass slurry is treated according to size and moisture regulations. Following separation of the gaseous, liquid, and solid phases, the determination of elemental, physical, and chemical fragments, yield of energy, and other properties of the various products is carried out. Homogeneous or heterogeneous catalysts improve the biocrude yield and quality as does higher moisture content. The high percentage of N2 and O2 in the feedstock results in lower quality products. This may be resolved through pre‐processing of the biomass, controlling of HTL processes conditions, and post‐upgrading processing of the HTL products. The promising potential of microalgae biomass for biocrude and drop‐in fuels may have an important contribution to the world's renewable energy sources through HTL in the near future.

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Production of a generic microbial feedstock for lignocellulose biorefineries through sequential bioprocessing

Cited by 15 publications

References 35 publications

Conversion of Exhausted Sugar Beet Pulp into Fermentable Sugars from a Biorefinery Approach

Conversion of Exhausted Sugar Beet Pulp into Fermentable Sugars from a Biorefinery Approach

Optimization of BS4 Enzyme production with Different Substrate Thickness and Type of Trays

Continuous Hydrothermal Liquefaction for Biofuel and Biocrude Production from Microalgal Feedstock

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