Production in high-yield of a naphthoquinone by a hairy root culture of Sesamum indicum

Ogasawara, Takeshi; Chiba, Kazuhiro; Tada, Mizuki

doi:10.1016/0031-9422(93)85029-q

Cited by 46 publications

(22 citation statements)

References 4 publications

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“…Somatic embryos have also been induced from hypocotyl-derived calluses, but no plant regeneration was achieved (Mary and Jayabalan 1997). Hairy root cultures using Agrobacterium rhizogenes have been established (Ogasawara et al 1993;Jin et al 2005). Sesame has been shown to be susceptible to A. tumefaciens, but no transformed shoot/plant was recovered (Taskin et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.)

Yadav

Chaudhary

Sainger

et al. 2010

Plant Cell Tiss Organ Cult

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Sesame (Sesamum indicum) is an important oil seed crop that has not yet been transformed genetically. We report herein for the first time successful recovery of fertile transgenic plants of sesame from cotyledon explants inoculated with Agrobacterium tumefaciens carrying a binary vector pCAMBIA2301 that contains a neomycin phosphotransferase gene (nptII) and a b-glucuronidase (GUS) gene (uidA) interrupted with an intron. Green shoots recovered from A. tumefaciens-infected explants on selection medium [Murashige and Skoog (MS) basal medium containing 25.0 lM benzyladenine (BA), 25.0 mg l -1 kanamycin and 400.0 mg l -1 cefotaxime] were rooted on MS basal medium containing 2.0 lM indole-3-butyric acid and 5.0 mg l -1 kanamycin. The rooted shoots were established in soil and grown to maturity to collect seeds. The presence, integration and expression of transgenes in putative T 0 plants were confirmed by polymerase chain reaction (PCR), Southern blot hybridization and GUS histochemical assay, respectively. GUS activity was detected in vegetative and reproductive parts of T 0 and T 1 plants. Presence of thiol compounds in coculture medium and kanamycin selection at shoot regeneration and at rooting stages were found to be critical for transformation. The transgenes were inherited in Mendelian fashion in T 1 progeny as detected by PCR. RT-PCR analysis of T 1 plants confirmed the presence of transcripts of uidA gene. The transformation frequency was 1.01%, and 22-24 weeks were required from seed to seed generation time. This protocol can be used to transfer new traits in sesame for quantitative and qualitative improvement.

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Section: Introductionmentioning

confidence: 99%

Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.)

Yadav

Chaudhary

Sainger

et al. 2010

Plant Cell Tiss Organ Cult

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“…1), have been isolated from the roots and hairy roots of S. indicum. [2][3][4] The carbon skeleton of MPAQ (5), which consists of a C 6 side-chain at C-2 in the anthraquinone ring, is identical with that of other anthraquinones, suggesting that these anthraquinones in sesame are biosynthesized on the same pathway. The side-chain in MPAQ (5), as well as the C 10 side-chain in 2-geranyl-1,4-naphthoquinone that is a plausible intermediate of these anthraquinones, originates from the methylerythritol phosphate pathway.…”

mentioning

confidence: 93%

Dehydroanthrasesamones A and B, Anthraquinone Derivatives Containing a Dienyl Side-Chain from <i>Sesamum indicum</i> Hairy Roots

Furumoto

Hoshikuma

2013

Bioscience, Biotechnology, and Biochemistry

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Two additional anthraquinone derivatives containing a (Z)-dienyl side-chain, dehydroanthrasesamones A (2) and B (3), were isolated from a hairy root culture of Sesamum indicum after preventing light throughout all experimental procedures. Their respective structures were determined to be 1-hydroxy-2-[(Z)-4-methylpenta-1,3-dien-1-yl]anthraquinone and 1,4-dihydroxy-2-[(Z)-4-methylpenta-1,3-dien-1-yl]anthraquinone by spectroscopic methods.

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“…et Zucc. (Köhle et al, 2002), Impatiens balsamina L. (Balsaminaceae) (Panichayupakaranant & De-Eknamkul, 1992), Plumbago rosea L. (Plumbaginaceae) (Panichayupakaranant & Tewtrakul, 2002;Gangopadhyay et al, 2008) and Sesamum indicum L. (Pedaliaceae) (Ogasawara et al, 1993), etc. This work was therefore focused on establishment of R. nasutus root cultures and determination of rhinacanthin production.…”

Section: Introductionmentioning

confidence: 99%

Effect of medium composition and light on root and rhinacanthin formation inRhinacanthus nasutuscultures

Panichayupakaranant¹,

Meerungrueang²

2010

Pharmaceutical Biology

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Production in high-yield of a naphthoquinone by a hairy root culture of Sesamum indicum

Cited by 46 publications

References 4 publications

Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.)

Agrobacterium tumefaciens-mediated genetic transformation of sesame (Sesamum indicum L.)

Dehydroanthrasesamones A and B, Anthraquinone Derivatives Containing a Dienyl Side-Chain from <i>Sesamum indicum</i> Hairy Roots

Effect of medium composition and light on root and rhinacanthin formation inRhinacanthus nasutuscultures

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