2006
DOI: 10.1590/s0074-02762006000800009
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Production, characterization, and application of antibodies against heat-labile type-I toxin for detection of enterotoxigenic Escherichia coli

Abstract: Strains of enterotoxigenic Escherichia coli (ETEC) are responsible for significant rates of morbidity and mortality among children, particularly in developing countries. The majority of clinical and public health laboratories are capable of isolating and identifying Salmonella, Shigella, Campylobacter, and Escherichia coli O157:H7 from stool samples, but ETEC cannot be identified by routine methods. The method most often used to identify ETEC is polymerase chain reaction for heat-stable and heat-labile enterot… Show more

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Cited by 22 publications
(20 citation statements)
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References 36 publications
(42 reference statements)
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“…Several immunological assays where LT is captured either by ganglioside GM1 (its receptor in the host cell) or by antibodies have been described 139, 321, 377, 378. Assays for ST by indirect ELISA using IgG1 ST-mAb and for LT by capture ELISA employing IgG enriched fraction of a rabbit polyclonal as a capture antibody and IgG2b LT-mAb as a second antibody have been employed as tools for diagnosis.…”
Section: Enterotoxigenic E Colimentioning
confidence: 99%
“…Several immunological assays where LT is captured either by ganglioside GM1 (its receptor in the host cell) or by antibodies have been described 139, 321, 377, 378. Assays for ST by indirect ELISA using IgG1 ST-mAb and for LT by capture ELISA employing IgG enriched fraction of a rabbit polyclonal as a capture antibody and IgG2b LT-mAb as a second antibody have been employed as tools for diagnosis.…”
Section: Enterotoxigenic E Colimentioning
confidence: 99%
“…The 3E2 clone was obtained in the first fusion after Stx1 treatment by glutaraldehyde and using the immunization and fusion protocols already described by our group either for the MAbs IgG2b anti-LT or IgG2b anti-intimin [50,51,52]. In contrast, the 2E11 clone was merely obtained after ten fusions, through different tested protocols either for toxoid conversion or route and schedule immunization.…”
Section: Discussionmentioning
confidence: 99%
“…Accordingly, a collection of LT-, ST- and ST/LT-producing ETEC isolates was used to evaluate different protocols for toxin production and release aimed at ETEC detection. For this detection, we used previously raised anti-LT antibodies [24,25] and anti-ST monoclonal and polyclonal antibodies described herein.…”
Section: Introductionmentioning
confidence: 99%