Production and characterization of polyhydroxyalkanoates from industrial waste using soil bacterial isolates

Shah, Shreya; Kumar, Anil

doi:10.1007/s42770-021-00452-z

Cited by 11 publications

(8 citation statements)

References 40 publications

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“…It has been pointed out that under nutritional stress conditions, bacterial cells with higher PHA contents survive longer than those with lower PHA contents (Tufail et al, 2017). PHA granules serve as a carbon and energy source during starvation and are crucial for bacterial survival under harsh conditions caused by 昀氀uctuating osmotic pressure and radiation (Urtuvia, 2014;Shah and Kumar, 2021). PHA degrades naturally and completely into carbon dioxide and water under aerobic conditions and into methane under anaerobic conditions (Tsuge et al, 2015;Tufail et al, 2017).…”

Section: Discussionmentioning

confidence: 99%

“…PHA degrades naturally and completely into carbon dioxide and water under aerobic conditions and into methane under anaerobic conditions (Tsuge et al, 2015;Tufail et al, 2017). In that way, PHA can be considered biodegradable plastics and they are less problematic than petroleum-based plastics for the environment (Urtuvia, 2014;Shah and Kumar, 2021).…”

Section: Discussionmentioning

confidence: 99%

“…These activities have directly or indirectly caused irreversible changes in microbial biodiversity. Resistance in PHA-producing bacteria could be a consequence of anthropogenic pollution observed at the di昀昀erent sampling points (Lupo et al, 2012;Shah and Kumar, 2021). It has been suggested (Blair et al, 2015;Hall and Mah, 2017) that in tropical environments, microbial populations resistant to antibiotics may be selected from the natural microbiota upon contact with these compounds.…”

Section: Discussionmentioning

confidence: 99%

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Aislamiento y caracterización de bacterias productoras de polihidroxialcanoato a partir de muestras de agua de mar (Tumaco)

Izquierdo

Villota-Calvachi

Otero-Ramírez

et al. 2023

Universitas Scientiarum

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Abstract. Introduction. Polyhydroxyalkanoate (PHA) is a useful, carbon-neutral polymer that can be produced from renewable carbon sources by microorganisms, making it a sustainable and environmentally friendly material. Currently, production of the biopolymer is not competitive in terms of cost and yield; however, production by different bacterial strains may provide economic viability, especially since substituting biodegradable plastics for nondegradable synthetic plastics has drawn interest from both academia and the commercial world. Objective. The aim of this work was to describe the isolation and partial characterization of PHA-producing bacteria isolated from five sites (gas stations) in coastal regions of Tumaco Island, Nariño-Colombia, and classify different physicochemical parameters of granules that show the production of the biopolymer. Materials and methods. A viable colony staining method using Nile red was used to preliminarily screen for PHA-producing bacteria. Colonies were isolated, characterized via biochemical, microbial, and molecular methods, and tested for antimicrobial susceptibility and fermentation. Results. More than thirty-eight strains were identified as potential PHA-positive isolates from this screening approach but, just one isolated was viable in PHA production (T2-25A). All isolates were resistant to metronidazole, ampicillin, trimethoprim sulfamethoxazole, cephalothin, ceftriaxone, and cefazolin, and 27.3 % of isolates were resistant to novobiocin. Conclusions. One promising PHA-producing isolate was obtained. Nevertheless, this information will complement future studies of the conditions necessary to produce PHA. Moreover, antibiotic resistance data have attracted attention, especially because of the origin of the source waters of the isolates.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

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Aislamiento y caracterización de bacterias productoras de polihidroxialcanoato a partir de muestras de agua de mar (Tumaco)

Izquierdo

Villota-Calvachi

Otero-Ramírez

et al. 2023

Universitas Scientiarum

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“…The special features that make them attractive biomedical materials are their biocompatibility biodegradability, and non-toxicity. PHAs suitable for many medical applications have been found for bio-control agents, drug carriers, biodegradable implants, tissue engineering, memory enhancers, and anticancer drugs [ 141 ]. The current PHA industry is affected by the high price of the carbon substrate.…”

Section: Glycerol Into Value-added Productsmentioning

confidence: 99%

Glycerol waste to value added products and its potential applications

Chilakamarry

Sakinah

Zularisam

et al. 2021

Syst Microbiol and Biomanuf

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Graphic abstract The rapid industrial and economic development runs on fossil fuel and other energy sources. Limited oil reserves, environmental issues, and high transportation costs lead towards carbon unbiased renewable and sustainable fuel. Compared to other carbon-based fuels, biodiesel is attracted worldwide as a biofuel for the reduction of global dependence on fossil fuels and the greenhouse effect. During biodiesel production, approximately 10% of glycerol is formed in the transesterification process in a biodiesel plant. The ditching of crude glycerol is important as it contains salt, free fatty acids, and methanol that cause contamination of soil and creates environmental challenges for researchers. However, the excessive cost of crude glycerol refining and market capacity encourage the biodiesel industries for developing a new idea for utilising and produced extra sources of income and treat biodiesel waste. This review focuses on the significance of crude glycerol in the value-added utilisation and conversion to bioethanol by a fermentation process and describes the opportunities of glycerol in various applications.

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“…Microbially produced polyhydroxyalkanoates (PHAs) have gained industrial interest as these polyesters constitute a sustainably produced, non‐toxic and biodegradable alternative to petroleum‐based plastics (Kovalcik et al, 2019 ). PHAs are biosynthesized by numerous prokaryotic microorganisms during unbalanced nutritional conditions, especially in case of a high carbon‐to‐nitrogen ratio and are accumulated in intracellular granules as a means of carbon and energy storage (Jendrossek, 2009 ; Shah & Kumar, 2021 ). In addition, PHA synthesis is stimulated in response to specific stress conditions, such as high salinity, and has been proposed to play a role in cellular protection during such stress conditions (Obruča et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Paracoccus kondratievae produces poly(3‐hydroxybutyrate) under elevated temperature conditions

Moanis,

Geeraert,

Van den Brande

et al. 2024

Environ Microbiol Rep

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As part of ongoing efforts to discover novel polyhydroxyalkanoate‐producing bacterial species, we embarked on characterizing the thermotolerant species, Paracoccus kondratievae, for biopolymer synthesis. Using traditional chemical and thermal characterization techniques, we found that P. kondratievae accumulates poly(3‐hydroxybutyrate) (PHB), reaching up to 46.8% of the cell's dry weight after a 24‐h incubation at 42°C. Although P. kondratievae is phylogenetically related to the prototypical polyhydroxyalkanoate producer, Paracoccus denitrificans, we observed significant differences in the PHB production dynamics between these two Paracoccus species. Notably, P. kondratievae can grow and produce PHB at elevated temperatures ranging from 42 to 47°C. Furthermore, P. kondratievae reaches its peak PHB content during the early stationary growth phase, specifically after 24 h of growth in a flask culture. This is then followed by a decline in the later stages of the stationary growth phase. The depolymerization observed in this growth phase is facilitated by the abundant presence of the PhaZ depolymerase enzyme associated with PHB granules. We observed the highest PHB levels when the cells were cultivated in a medium with glycerol as the sole carbon source and a carbon‐to‐nitrogen ratio of 10. Finally, we found that PHB production is induced as an osmotic stress response, similar to other polyhydroxyalkanoate‐producing species.

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Production and characterization of polyhydroxyalkanoates from industrial waste using soil bacterial isolates

Cited by 11 publications

References 40 publications

Aislamiento y caracterización de bacterias productoras de polihidroxialcanoato a partir de muestras de agua de mar (Tumaco)

Aislamiento y caracterización de bacterias productoras de polihidroxialcanoato a partir de muestras de agua de mar (Tumaco)

Glycerol waste to value added products and its potential applications

Paracoccus kondratievae produces poly(3‐hydroxybutyrate) under elevated temperature conditions

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