2010
DOI: 10.1128/aem.02558-09
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Abstract: A bacterium producing an extracellular bioflocculant was isolated from contaminated LB medium and identified as Bacillus licheniformis by 16S rRNA gene sequencing and its biochemical/physiological characteristics. The optimum culture conditions for flocculant production were an initial medium pH of 7.2 and an inoculum size of 4% (vol/vol). The maximum flocculating activity (700 U/ml) was obtained after cultivation at 37°C for 48 h. Chemical analyses of the purified bioflocculant revealed that it was a proteogl… Show more

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Cited by 146 publications
(147 citation statements)
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“…These results are in agreement with the phenomenon observed by Salehizadeh and Shojaosadati [49] that a small inoculum prolonged the lag phase of the strain, whereas a large inoculum made the niche of Bacillus pumilus strain overlap excessively and restrained bioflocculant production which means that at large inoculum size, the substrates in the production medium got depleted faster within a shorter period as a result of the bacterial population in the culture medium, generating high turbidity at an early stage of growth and this consequently affects bioflocculant production. A similar finding was reported by Xiong et al [35] where it was observed that an inoculum size of 4% (v/v) was optimum for bioflocculant production by Bacillus licherniformis.…”
Section: Effect Of Initial Ph Of Production Medium On Bioflocculant Psupporting
confidence: 75%
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“…These results are in agreement with the phenomenon observed by Salehizadeh and Shojaosadati [49] that a small inoculum prolonged the lag phase of the strain, whereas a large inoculum made the niche of Bacillus pumilus strain overlap excessively and restrained bioflocculant production which means that at large inoculum size, the substrates in the production medium got depleted faster within a shorter period as a result of the bacterial population in the culture medium, generating high turbidity at an early stage of growth and this consequently affects bioflocculant production. A similar finding was reported by Xiong et al [35] where it was observed that an inoculum size of 4% (v/v) was optimum for bioflocculant production by Bacillus licherniformis.…”
Section: Effect Of Initial Ph Of Production Medium On Bioflocculant Psupporting
confidence: 75%
“…In bioflocculation, a number of Bacillus species have been documented for their bioflocculant-producing potential, including B. licherniformis [35], B. mucilaginosus [36,37], B. circulans [38], B. subtilis [39] and B. alvei [40]. However, Bacillus pumilus has not been reported in literature as a bioflocculant producer.…”
Section: Isolation Of Bioflocculant-producing Bacteriamentioning
confidence: 99%
“…The weak stretching band at ~ 2955/cm known to be typical of carbohydrates, indicated COH asymmetrical stretching vibration. An asymmetrical stretching peak observed at ~1,640/cm was characteristic of CAO stretching vibration in ONHCOCH3 (Xiong et al, 2010). The weak band near 1405/cm indicates the bending vibration of CH 3 and the scissor vibration of CH 2 .…”
Section: Characterization Of Bioflocculantsmentioning
confidence: 98%
“…The Fourier-transform Infra-red (FTIR) spectra (Fig. 3 a, b and c) of the three bioflocculants were analyzed according to Xiong et al (2010). It clearly showed the presence of carboxyl, hydroxyl, amide and amino groups in its molecules, which are the preferred groups for the flocculation, similar to those observed in polyelectrolytes (Ganesh- Kumar et al, 2004).…”
Section: Characterization Of Bioflocculantsmentioning
confidence: 99%
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