Abstract. The aim of the current study was to investigate the effect of the main thermal treatments used in practice by beekeepers and packagers on hydroxymethylfurfural content and diastase activity of Cretan honey. Results showed a significant alteration of both quality parameters under heating at 65 °C for 6 h (P<0.05) while heating at 45 °C for 24 h was found to be the least severe treatment with regards to the variation of the two quality parameters (P<0.05). After heating, a significant differentiation was also observed in the variation of both HMF content and diastase activity according to the botanical origin of the honey sample (P<0.05). Pine honey was the most resistant sample in hydroxymethylfurfural formation in all heating procedures and multifloral honey was the least altered in its enzymatic activity through the whole thermal process.Keywords: Honey, thermal treatment, botanical origin, HMF, diastase activity.
IntroductionHoney is composed of a complex mixture of water, carbohydrates and numerous other minor compounds. In fact, it includes more than 400 different substances including organic acids, proteins, enzymes, aroma substances, mineral substances, pigments and waxes [1]. Considering the interest generated by all the properties associated with honey, several processing operations have been introduced to ensure a safe, pleasant and homogeneous presentation of the final product [1]. These processing steps generally require exposure to heat in order to reduce viscosity and to prevent crystallization or fermentation of the honey [1]. Gonnet et al [1] were the first to suggest heating at 78 °C for 6-7 min as the best pasteurizing condition to avoid damage to the quality of honey. Since then, a wide range of heating temperatures ranging from 30 to 140 °C for a few seconds up to several hours have been practiced by honey producers worldwide, with the aim to reduce the water content in honey below 20% for shelf life prolongation [1,2,3]. Subramanian [1] suggested that conventional honey processing includes preheating (to about 40 °C), straining, clarification by means of filtration and heating of the product at 60-65 °C for 25-30 min. In industrial plants, heating is done in special large surface heat exchange systems used with a heat source only a few degrees above the temperature to which the honey is to be heated [1]. However, an uncontrolled thermal processing of honey may also result in product quality deterioration and even in caramelization due to significant losses that occur in color, texture, flavor, enzymatic activity and nutritive value. In fact, its low thermal conductivity makes uniform heating throughout a large body of honey very difficult and the use of high temperature heat sources like open flames or boiling water baths may quickly lead to significant alterations of the desirable characteristics of honey.Hydroxymethylfurfural (HMF) content and diastase activity are quality parameters that are heatsensitive as they are increasing or decreasing respectively according to the intensit...