Abstract:Summary
Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34
+
progenitor and more differentiated CD34
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fractions from post-natal thymic tissue to study thymopoiesis. CD34
+
cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched C… Show more
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