volume 1, issue 2, P100090 2020
DOI: 10.1016/j.xpro.2020.100090
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Abstract: Summary Single cell RNA sequencing of human thymic cells is dependent on isolation of highly pure and viable cell populations. This protocol describes the isolation of CD34 + progenitor and more differentiated CD34 – fractions from post-natal thymic tissue to study thymopoiesis. CD34 + cells represent <1% of thymic cells, so this protocol uses magnetic- followed by fluorescence-activated cell separation to isolate highly enriched C…

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