Process Intensification Based on Disposable Solutions as First Step Toward Continuous Processing

Towards continuous mAb purification: Clearance of host cell proteins from CHO cell culture harvests via “flow‐through affinity chromatography” using peptide‐based adsorbents

“…Adhere resin in series afforded a yield of 87.2% and an HCP LRV of 2.75. et al, 2018) while may also substantially reduce the cost of production and make LigaGuard™ adsorbent potentially disposable and single-use (Schmidt, 2022). For continuous or hybrid manufacturing, we envision that multiple LigaGuard™ columns can be installed in parallel trains, similarly to other membrane-and resin-based chromatographic adsorbents utilized in continuous-ready processes (Mahal et al, 2021).…”

“…Future efforts on the LigaGuard™ technology will aim at evaluating the HCP‐binding activity of the single peptide ligands, with the goal of removing the sequences with lesser contribution than the rest and minimizing the number of ligands forming the LigaGuard™ ensemble. In addition, we are exploring the use of inexpensive chromatographic substrates, such as silica, which exhibit robust properties (C. Zhang et al, 2018) while may also substantially reduce the cost of production and make LigaGuard™ adsorbent potentially disposable and single‐use (Schmidt, 2022). For continuous or hybrid manufacturing, we envision that multiple LigaGuard™ columns can be installed in parallel trains, similarly to other membrane‐ and resin‐based chromatographic adsorbents utilized in continuous‐ready processes (Mahal et al, 2021).…”