Probiotic Potential of Bacillus licheniformis and Bacillus pumilus Isolated from Tibetan Yaks, China

Zeng, Zhibo; Zhang, Jiabin; Li, Yan; Li, Kewei; Gong, Saisai; Li, Feiran; Wang, Pengpeng; Iqbal, Mudassar; Kulyar, Muhammad Fakhar‐e‐Alam; Li, Jiakui

doi:10.1007/s12602-022-09939-z

Cited by 19 publications

(10 citation statements)

References 74 publications

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“…Wang et al reported that Salmonella infection in mice caused liver injury and inflammatory response; the mRNA levels of pro-inflammatory factors (IL-1β, IL-6, TNF-α, and IFN-γ) and chemokines (CCL2 and CCL3) in the liver increased significantly. In the present study, TS1 also reduced the SE-induced increase in the expression of pro-inflammatory cytokines (IL-1β, IL-6, TNF-α, IL-4, MCP-1) in the intestine and serum, which is consistent with previous findings and observations of lipopolysaccharide-induced enteritis in vitro [45,48].…”

Section: Discussionsupporting

confidence: 94%

“…Asterisks indicate significant differences ( ns P > 0.05, *P < 0.05, and **P < 0.01) between the control (con) and treatment groups results suggest that TS1 reduced the SE-induced increase in MDA content but enhanced the activity of SOD and GSH-Px and increased T-AOC. These findings are consistent with previous reports and indicate that TS1 protected poultry intestinal tissue from SE-induced damage by enhancing the function of the antioxidant system [45]. Oxidative stress causes over-inflammation, which leads to cell death or activates several apoptosis signaling pathways [46], which causes significant intestinal inflammatory cell infiltration, crypt loss, and epithelial cell destruction [47].…”

Section: Discussionsupporting

confidence: 92%

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Bacillus pumilus TS1 alleviates Salmonella Enteritidis-induced intestinal injury in broilers

Liu

et al. 2023

BMC Vet Res

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Background In the current context of reduced and limited antibiotic use, several pathogens and stressors cause intestinal oxidative stress in poultry, which leads to a reduced feed intake, slow or stagnant growth and development, and even death, resulting in huge economic losses to the poultry breeding industry. Oxidative stress in animals is a non-specific injury for which no targeted drug therapy is available; however, the health of poultry can be improved by adding appropriate feed additives. Bacillus pumilus, as a feed additive, promotes growth and development and reduces intestinal oxidative stress damage in poultry. Heat shock protein 70 (HSP70) senses oxidative damage and repairs unfolded and misfolded proteins; its protective effect has been widely investigated. Mitogen-activated protein kinase/protein kinase C (MAPK/PKC) and hypoxia inducible factor-1 alpha (HIF-1α) are also common proteins associated with inflammatory response induced by several stressors, but there is limited research on these proteins in the context of poultry intestinal Salmonella Enteritidis (SE) infections. In the present study, we isolated a novel strain of Bacillus pumilus with excellent performance from the feces of healthy yaks, named TS1. To investigate the effect of TS1 on SE-induced enteritis in broilers, 120 6-day-old white-feathered broilers were randomly divided into four groups (con, TS1, SE, TS1 + SE). TS1 and TS1 + SE group chickens were fed with 1.4 × 107 colony-forming units per mL of TS1 for 15 days and intraperitoneally injected with SE to establish the oxidative stress model. Then, we investigated whether TS1 protects the intestine of SE-treated broiler chickens using inflammatory cytokine gene expression analysis, stress protein quantification, antioxidant quantification, and histopathological analysis. Results The TS1 + SE group showed lower MDA and higher GSH-Px, SOD, and T-AOC than the SE group. TS1 alleviated the effects of SE on intestinal villus length and crypt depth. Our results suggest that SE exposure increased the expression of inflammatory factors (IL-1β, IL-6, TNF-α, IL-4, and MCP-1), p38 MAPK, and PKCβ and decreased the expression of HSP60, HSP70, and HIF-1α, whereas TS1 alleviated these effects. Conclusions Bacillus pumilus TS1 alleviated oxidative stress damage caused by SE and attenuated the inflammatory response in broilers through MAPK/PKC regulation of HSPs/HIF-1α.

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Section: Discussionsupporting

confidence: 94%

Section: Discussionsupporting

confidence: 92%

Bacillus pumilus TS1 alleviates Salmonella Enteritidis-induced intestinal injury in broilers

Liu

et al. 2023

BMC Vet Res

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“…The antioxidant capacity of strains is an essential element in assessing the beneficial potential of probiotics (Zeng et al 2022). According to our findings, the DPPH radical scavenging ability of the four strains was generally high, ranging from 85.36-67.32% (Fig.…”

Section: Antioxidant Activitymentioning

confidence: 60%

“…For successful influence and colonization of the host's small intestine, potential probiotic strains must be able to tolerate the low pH environment of the stomach (approximately 2.0-3.0). After the ingestion of food, the pH of gastric juice is usually 3.0, while the density of bile in the small intestine is typically between 0.1 and 0.3% (Liu et al 2013;Zeng et al 2022). In the present study, we assessed the tolerance of LAB strains isolated from yak intestines at pH 3.0 and 0.3% bile salt conditions.…”

Section: Discussionmentioning

confidence: 99%

Probiotic potential of lactic acid bacteria isolated from yaks

He,

Li,

et al. 2024

Animal Diseases

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The prevalence of bacterial digestive diseases in plateau animals has caused considerable losses to the Tibetan livestock industry. Therefore, this study aimed to isolate safe lactic acid bacteria (LAB) with beneficial probiotic properties to protect yaks from intestinal diseases. After 16S rDNA matching, four strains of Lactobacillus fermentum (A4), Pediococcus pentosaceus (A3.4 and A1.2), and Pediococcus acidilactici (B1.9) were isolated from the intestinal tissues and content of healthy yaks. The results indicated that A4 was more tolerant to bile salt (0.3%), while A3.4 had better stability in an acidic (pH = 3.0) environment. The results of the antibacterial activity test suggested that the isolates inhibited most pathogenic bacteria by up to 20%, except for A3.4, which inhibited Pasteurella and Staphylococcus aureus by more than 20%. Moreover, the results of the antioxidant test demonstrated that A4 and A3.4 had potent antioxidant activity. In addition, the drug sensitivity test revealed that the isolates were susceptible to commonly used antibiotics. In terms of safety, the isolates promoted growth, enhanced intestinal development, and protected the intestinal barrier without causing any adverse effects. In conclusion, LAB isolated from yak intestinal contents are potential probiotics with excellent antibacterial properties.

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“…The previous study [ 30 ] used the acid, bile, and trypsin to examine the resistance of probiotics candidates. 1% of activated strains was incubated in LB or MRS broth with pH (2.5, 3.5, 4.5), bile salts (0.2%, 0.5%, 0.8%), or trypsin (0.2%, 0.3%, 0.4%).…”

Section: Methodsmentioning

confidence: 99%

Screening of Bacteria Inhibiting Clostridium perfringens and Assessment of Their Beneficial Effects In Vitro and In Vivo with Whole Genome Sequencing Analysis

Jiang

Yang

et al. 2022

Microorganisms

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Various countries and organizations call for banning the use of antibiotic growth promoters (AGPs) as prophylaxis and for growth promotion in the livestock industry. Hence, seeking a substitute for antibiotics is strongly required by the livestock industry to maintain the productivity level and profits. Probiotics could represent one viable solution because of their beneficial effects on host health and maintaining the intestinal microbiota balance. In the present study, we aimed to isolate bacterial strains with probiotics properties from JinHua pig (a Chinese native pig breed) gastrointestinal tract that have antagonistic activity against to common disease-causing bacteria on farms. The four most potent strains were isolated (PP31, BA11, BA40, BV5) by the agar well diffusion method and further characterized by acid, bile salt, trypsin tolerance, whole genome sequencing (WGS), and suppressing Clostridium perfringens adhesion to IPEC-J2 cells. According to these results, BA40 had the highest number and variety of probiotic secondary metabolic secretion genes and capacity to exclude the attachment of Clostridium perfringens to IPEC-J2 cells as same as PB6. The animal experiment in vivo illustrated that BA40 and PB6 could reduce the phenomenon induced by Clostridium perfringens challenge of body weight loss, colon length decrease, pro-inflammatory cytokine increase, and Clostridium perfringens and Escherichia coli increase. The present study provides evidence that BA40 could represent a novel probiotic candidate as PB6, which exhibited some probiotic features and mitigated the burden of Clostridium perfringens associated gut disease.

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Probiotic Potential of Bacillus licheniformis and Bacillus pumilus Isolated from Tibetan Yaks, China

Cited by 19 publications

References 74 publications

Bacillus pumilus TS1 alleviates Salmonella Enteritidis-induced intestinal injury in broilers

Bacillus pumilus TS1 alleviates Salmonella Enteritidis-induced intestinal injury in broilers

Probiotic potential of lactic acid bacteria isolated from yaks

Screening of Bacteria Inhibiting Clostridium perfringens and Assessment of Their Beneficial Effects In Vitro and In Vivo with Whole Genome Sequencing Analysis

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