Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries‐inducing Streptococcus mutans

Wasfi, Reham; El‐Rahman, Ola A. Abd; Zafer, Mai M.; Ashour, Hossam M.

doi:10.1111/jcmm.13496

Cited by 159 publications

(100 citation statements)

References 68 publications

(86 reference statements)

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“…According to the SEM analysis, L. paracasei 25.4 (the strain with the greatest reduction in CFU/mL count) reduced the adhesion of S. mutans cells to the discs, and this effect probably caused the reduction in the CFU count. The images obtained in this study are in agreement with Wasfi et al [37], who investigated whether L. casei (ATCC 393), L. reuteri (ATCC 23272), L. plantarum (ATCC 14917) or L. salivarius (ATCC 11741) inhibit S. mutans biofilms. In their SEM images, the authors observed changes in the extracellular matrix, fewer bacteria and smaller microcolonies in biofilms formed during coculture of S. mutans and Lactobacillus.…”

Section: Discussionsupporting

confidence: 91%

Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Rossoni

Velloso

Barros

et al. 2018

Microbial Pathogenesis

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Probiotics can release bioactive substances that can inhibit the growth and biofilm formation of pathogenic microorganisms such as Streptococcus mutans. In this context, we evaluated whether the supernatants of Lactobacillus strains isolated from caries-free subjects can inhibit S. mutans, one of the most important bacteria for dental caries. First, the supernatants of 22 Lactobacillus strains were screened for antibacterial activity against S. mutans in planktonic cultures. All 22 Lactobacillus strains studied (100%) showed antibacterial activity. Thereafter, the Lactobacillus strains with the greatest reductions in the planktonic S. mutans cultures were tested on biofilms. The L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 strains could significantly reduce the number of S. mutans cells in biofilms formed in hydroxyapatite (p < 0.05). This reduction was also confirmed by scanning electron microscopy analysis and was not caused by the decreased pH value in the medium (p > 0.05). In addition, the supernatants of these probiotic strains could also reduce the total biomass of S. mutans biofilms (p < 0.05). In conclusion, most of the Lactobacillus strains tested have some antibacterial activity against S. mutans. L. fermentum 20.4, L. paracasei 11.6, L. paracasei 20.3 and L. paracasei 25.4 produce bioactive substances that caused a significant reduction in S. mutans biofilms.

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Section: Discussionsupporting

confidence: 91%

Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Rossoni

Velloso

Barros

et al. 2018

Microbial Pathogenesis

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“…It was observed that the neutralized CFS BioMed Research International (CFN) caused a lower reduction in microbial growth than CFS, but CFN still showed a significant reduction in G. vaginalis growth when compared to the control. Wasfi et al found that when CFS was treated to pH = 7, the ability of all 4 Lactobacillus inhibiting the growth of Streptococcus mutans is significantly declining (P < 0:01) [40]. Our results of the four L. plantarum are consistent with the results.…”

Section: Discussionsupporting

confidence: 90%

Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis

Qian

Zhao

et al. 2020

BioMed Research International

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Worldwide interest in the use of functional foods containing probiotic bacteria such as Lactobacillus and Bifidobacterium for health promotion and disease prevention has increased significantly. Probiotics have demonstrated beneficial properties including strengthening the body’s natural defense system, inhibiting the growth of pathogenic bacteria, and regulating mental activity, but their effects on the human vagina have not been fully elucidated. The primary purpose of our study was to isolate Lactobacillus strains from old yogurt, a traditional dairy product, and investigate their probiotic potential with respect to the human vaginal system. Four Lactobacillus plantarum (L. plantarum) strains, named ZX1, ZX2, ZX27, and ZX69, were isolated from the yogurt samples. Simultaneously, we used a commercial Lactobacillus strain (Lactobacillus delbrueckii DM8909) as a control strain. We tested the antimicrobial activity of Lactobacillus isolates against Escherichia coli and Gardnerella vaginalis by agar spot and well diffusion tests. Then, we tested the antibiotic susceptibility of the 5 strains by using the minimal inhibitory concentration method. We attempted to detect possible bacteriocin genes by PCR sequencing technique. Using a chemically defined medium simulating genital tract secretions, we found that the selected Lactobacillus strains could alter the expression of known virulence genes in Gardnerella vaginalis. Bacteriocins derived from these isolated strains had potent antibacterial activity against G. vaginalis and E. coli, with the most effective activity observed in the case of ZX27. In addition, all strains including the L. delbrueckii DM8909 were positive for the presence of the plantaricin cluster of genes described in L. plantarum C11. The tested stains possessed the pln gene indicating that one of the antibacterial agents was plantaricin. We assume that the production of antimicrobial substances such as bacteriocins induce G. vaginalis to upregulate antimicrobial resistance genes. The new isolated strains have bacteriocin-related genes and can change the antimicrobial resistance gene transcription of G. vaginalis.

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“…Additionally, by producing organic acid and peroxide, probiotics led to a decline in cell adherence and preformed biofilm. 42 Moreover, EPS produced by L. acidophilus A4 considerably could inhibit biofilm formation of E. coli O157: H7 by reducing the expression of genes related to chemotaxis (cheY) and curli formation (csgA, csgB and crl). 29 Burton et al clarified a mechanism of biofilm inhibition of C. albicans using the combination of L. plantarum SD5870, Streptococcus salivarius DSM 14685 and L. helveticus CBS N116411.…”

Section: Probiotics Influence Gene Expression Of Pathogenic Biofilmsmentioning

confidence: 99%

“…against the formation of biofilm and gene expression of S. mutans was studied. 42 Comelli et al selected the dairy probiotics that were capable of reducing the carcinogenicity of dental plaque. They showed that Lactococcus lactis NCC2211, as a nonpathogenic dairy probiotic, could be incorporated into a biofilm; so, imitating the dental plaque and it could be able to modify the growth of the cariogenic S. sobrinus OMZ176.…”

Section: The Activity Of Probiotics Against Different Types Of Clinicmentioning

confidence: 99%

The Battle of Probiotics and Their Derivatives Against Biofilms

Barzegari¹,

Kheyrolahzadeh²,

Khatibi³

et al. 2020

IDR

143

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Biofilm-related infections have been a major clinical problem and include chronic infections, device-related infections and malfunction of medical devices. Since biofilms are not fully available for the human immune system and antibiotics, they are difficult to eradicate and control; therefore, imposing a global threat to human health. There have been avenues to tackle biofilms largely based on the disruption of their adhesion and maturation. Nowadays, the use of probiotics and their derivatives has gained a growing interest in battling against pathogenic biofilms. In the present review, we have a close look at probiotics with the ultimate objective of inhibiting biofilm formation and maturation. Overall, insights into the mechanisms by which probiotics and their derivatives can be used in the management of biofilm infections would be warranted.

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Probiotic Lactobacillus sp. inhibit growth, biofilm formation and gene expression of caries‐inducing Streptococcus mutans

Cited by 159 publications

References 68 publications

Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Inhibitory effect of probiotic Lactobacillus supernatants from the oral cavity on Streptococcus mutans biofilms

Antibacterial Activity of Lactobacillus Strains Isolated from Mongolian Yogurt against Gardnerella vaginalis

<p>The Battle of Probiotics and Their Derivatives Against Biofilms</p>

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