Probiotic cellulose: Antibiotic-free biomaterials with enhanced antibacterial activity

Sabio, Laura; González, Ana; Ramírez-Rodríguez, Gloria Belén; Gutiérrez‐Fernández, José; Bañuelo, Oscar; Olivares, Mónica; Gálvez, Natividad; Delgado-López, José Manuel; Domínguez‐Vera, José M.

doi:10.1016/j.actbio.2021.01.039

Cited by 27 publications

(35 citation statements)

References 42 publications

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“…Likewise, BC has been evaluated for enzyme immobilization with relatively low efficiency, hence necessitating activation techniques to modify its sophisticated three-dimensional structure [ 52 , 53 ]. In addition, the suitability of BC to be used as a potential cell immobilization matrix with unique properties was recently reported [ 19 , 54 ]. However, scientific data on the behavior and physiology of bacteria in the immobilized state are still limited compared to natural environments, where cells usually adhere to surfaces, entailing biofilm formation.…”

Section: Discussionmentioning

confidence: 99%

Novel Probiotic/Bacterial Cellulose Biocatalyst for the Development of Functional Dairy Beverage

Lappa

Kachrimanidou

Alexandri

et al. 2022

Foods

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The development of innovative functional products with potential health benefits, under the concept of bio-economy, is flourishing. This study undertook an evaluation of non-dairy lactobacilli Lactiplantibacillus pentosus B329 and Lactiplantibacillus plantarum 820 as “ready to use” starter cultures. Lactic acid bacteria (LAB) cultures were evaluated for their fermentation efficiency, before and after freeze-drying, using cheese whey (CW) as a fermentation substrate and subsequent immobilization on bacteria cellulose (BC) to produce a novel biocatalyst. The biocatalyst was applied in functional sour milk production and compared with free cells via the assessment of physicochemical and microbiological properties and sensory evaluation. Evidently, LAB strains exhibited high fermentative activity before and after freeze-drying. Results of a 5-month storage stability test showed that viability was 19% enhanced by immobilization on BC, supporting the concept of “ready to use” cultures for the production of fermented beverages. Likewise, sour milk produced by the BC biocatalyst presented higher organoleptic scores, compared to the free cells case, whereas immobilization on BC enhanced probiotic viability during post-fermentation storage (4 °C, 28 days). The obtained high viability (>107 log cfu/g) demonstrated the efficacy of the proposed bioprocess for the production of functional/probiotic-rich beverages. Ultimately, this work presents a consolidated scheme that includes the advantages and the cooperative effect of probiotic LAB strains combined with a functional biopolymer (BC) towards the formulation of novel functional products that coincide with the pillars of food systems sustainability.

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Section: Discussionmentioning

confidence: 99%

Novel Probiotic/Bacterial Cellulose Biocatalyst for the Development of Functional Dairy Beverage

Lappa

Kachrimanidou

Alexandri

et al. 2022

Foods

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“…The protocol used to quantify the number of probiotics adsorbed on the BC pellicles was recently described [ 26 ]. Briefly, functionalized BC (0.15 g) was digested with cellulase from Trichoderma reesei (No C2730-50ML, Sigma–Aldrich).…”

Section: Methodsmentioning

confidence: 99%

“…Roig-Sanchez et al have recently reported that, in fact, layers of BC can be decorated step-by-step with different nanoparticles (Ag and TiO 2 , among others), thus creating a mille-feuille concept of multifunctional BC [ 24 ]. On the other hand, we have recently demonstrated that BC can incorporate a huge number of probiotics resulting in a living biomaterial with enhanced antibacterial activity [ 26 ].…”

Section: Mainmentioning

confidence: 99%

Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles

et al. 2021

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The constant increase of antibiotic-resistant bacteria demands the design of novel antibiotic-free materials. The combination of antibacterials in a biocompatible biomaterial is a very promising strategy to treat infections caused by a broader spectrum of resistant pathogens. Here, we combined two antibacterials, silver nanoparticles (AgNPs) and living probiotics (Lactobacillus fermentum, Lf), using bacterial cellulose (BC) as scaffold. By controlling the loading of each antibacterial at opposite BC sides, we obtained a two-sided biomaterial (AgNP-BC-Lf) with a high density of alive and metabolically active probiotics on one surface and AgNPs on the opposite one, being probiotics well preserved from the killer effect of AgNPs. The resulting two-sided biomaterial was characterized by Field-Emission Scanning Electron Microscopy (FESEM) and Confocal Laser Scanning Microscopy (CLSM). The antibacterial capacity against Pseudomonas aeruginosa (PA), an opportunistic pathogen responsible for a broad range of skin infections, was also assessed by agar diffusion tests in pathogen-favorable media. Results showed an enhanced activity against PA when both antibacterials were combined into BC (AgNP-BC-Lf) with respect to BC containing only one of the antibacterials, BC-Lf or AgNP-BC. Therefore, AgNP-BC-Lf is an antibiotic-free biomaterial that can be useful for the therapy of topical bacterial infections.

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“…Cellulose is a biocompatible, biodegradable natural polymer, which can be functionalized, and its functionalized derivatives can be used as wound dressing material, whereby the loading of such dressings with antimicrobials infections in chronic wounds can be controlled, and the effectiveness against drug-resistant bacteria was also observed [ 137 , 138 , 139 ]. An antibiotic-free biomaterial, probiotic cellulose composite consisting of alive but also metabolically active probiotics Lactobacillus fermentum or Lactobacillus gasseri within the cellulose matrix showed excellent antibacterial activity against S. aureus , P. aeruginosa and MRSA and can be used instead of antibiotics for the treatment of topical infections, including severe chronic wounds [ 236 ]. Recent progress in the preparation of nanocellulose-based antimicrobial materials containing various functional groups, including aldehyde groups, NH 4 + , metal/metaloxide NPs and CS, showing potential to be used as wound dressings and drug carriers, was summarized by Norrrahim et al [ 237 ] The increasing content of aldehyde groups the dialdehyde nanocrystalline cellulose showed superb antibacterial activity against Gram-positive pathogens in vitro and pronouncedly decreased the amount of MRSA on the skin of infected mice models.…”

Section: Applied Nanomaterialsmentioning

confidence: 99%

Advances in Nanostructures for Antimicrobial Therapy

Jampílek

Kráľová

2022

Materials

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Microbial infections caused by a variety of drug-resistant microorganisms are more common, but there are fewer and fewer approved new antimicrobial chemotherapeutics for systemic administration capable of acting against these resistant infectious pathogens. Formulation innovations of existing drugs are gaining prominence, while the application of nanotechnologies is a useful alternative for improving/increasing the effect of existing antimicrobial drugs. Nanomaterials represent one of the possible strategies to address this unfortunate situation. This review aims to summarize the most current results of nanoformulations of antibiotics and antibacterial active nanomaterials. Nanoformulations of antimicrobial peptides, synergistic combinations of antimicrobial-active agents with nitric oxide donors or combinations of small organic molecules or polymers with metals, metal oxides or metalloids are discussed as well. The mechanisms of actions of selected nanoformulations, including systems with magnetic, photothermal or photodynamic effects, are briefly described.

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Probiotic cellulose: Antibiotic-free biomaterials with enhanced antibacterial activity

Cited by 27 publications

References 42 publications

Novel Probiotic/Bacterial Cellulose Biocatalyst for the Development of Functional Dairy Beverage

Novel Probiotic/Bacterial Cellulose Biocatalyst for the Development of Functional Dairy Beverage

Two-Sided Antibacterial Cellulose Combining Probiotics and Silver Nanoparticles

Advances in Nanostructures for Antimicrobial Therapy

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