Probing the Intercalation of Noscapine from Sodium Dodecyl Sulfate Micelles to Calf Thymus Deoxyribose Nucleic Acid: A Mechanistic Approach

Abstract

Noscapine (NOS) is efficient in inhibiting cellular proliferation and induces apoptosis in nonsmall cell, lung, breast, lymphatic, and prostate cancers. The micelle-assisted drug delivery is a well-known phenomenon; however, the proper mechanism is still unclear. Therefore, in the present study, we have shown a mechanistic approach for the delivery of NOS from sodium dodecyl sulfate (SDS) micelles to calf thymus deoxyribose nucleic acid (ctDNA) base-pairs using various spectroscopic techniques. The absorption and emission spectroscopy results revealed that NOS interacts with the SDS micelle and resides in its hydrophobic core. Further, the intercalation of NOS from SDS micelles to ctDNA was also shown by these techniques. The anisotropy and quenching results further confirmed the relocation of NOS from SDS micelles to ctDNA. The CD analysis suggested that SDS micelles do not perturb the structure of ctDNA, which supported that SDS micelles can be used as a safe delivery vehicle for NOS. This work may be helpful for the invention of advanced micelle-based vehicles for the delivery of an anticancer drug to their specific target site.

Insight into Drug Loading Regulated Micellar Rigidity by Nuclear Magnetic Resonance

“…Subsequently, the steady state anisotropy (r 0 ) measurement was applied to give insight into the micellar microenvironment (i.e., rigidity) around the fluorophore RhB. 26,27 The r 0 values of RhB in LL, ML, and HL micelles are 0.04, 0.11, and 0.12, respectively (Figure 4b), among which HL micelles possess the highest micellar rigidity and the most restricted microenviron- ment owing to the high drug loading boosted drug−polymer interaction, which corroborates well with the AFM results. Moreover, Fourier transform-infrared spectroscopy (FT-IR) was applied to elucidate the drug−polymer interaction and molecular self-assembly of IMC@F127 micelles (Figure 4c).…”

New Apoptosis Inducers Containing Anti-inflammatory Drugs and Pnictogen Derivatives: A New Strategy in the Development of Mitochondrial Targeting Chemotherapeutics

“…6 It also causes cell cycle arrest in either the S or G 2 /M phase depending on both p53 status and drug concentration. 38 Thus, conjugates 1−4 and 21.9% (1), 14.6% (2), 13.9% (3), 29.8% (4), 35.6% (SLS@1), 13.1% (SLS@2), 23.1% (SLS@3), and 43.9% (SLS@4). The cell populations in the S phase are 3.1% (control group), 9.6% (1), 12.4% (2), 20.2% (3), 12.0% (4), 11.6% (SLS@1), 16.0% (SLS@2), 15.0% (SLS@3), and 15.1% (SLS@4).…”

“…The relative number of cells within each cell cycle was determined by flow cytometry. The cell populations in the sub-G 1 phase are 5.7% (control group),21.9% (1), 14.6% (2), 13.9% (3), 29.8% (4), 35.6% (SLS@1), 13.1% (SLS@2), 23.1% (SLS@3), and 43.9% (SLS@4). The cell populations in the S phase are 3.1% (control group), 9.6% (1), 12.4% (2), 20.2% (3), 12.0% (4), 11.6% (SLS@1), 16.0% (SLS@2), 15.0% (SLS@3), and 15.1% (SLS@4).…”

DFT and docking studies of designed conjugates of noscapines & repurposing drugs: promising inhibitors of main protease of SARS-CoV-2 and falcipan-2

“…Heterocyclic compounds are versatile biological applications like anti-microbial, anti-diabetic, anti-malarial etc. Noscapine is a isoquinoline based molecules and reported as cough suppressant, anti-malarial and anti-cancerous (Kumar et al, 2020dMaurya, Alzahrani, et al, 2019;. Previously, the group has reported the noscapines as potential inhibitors of main protease of SARS-CoV-2 using molecular docking and molecular dynamics simulations.…”