Probing the Antitumor Mechanism of Solanum nigrum L. Aqueous Extract against Human Breast Cancer MCF7 Cells

Ling, Binbing; Xiao, Shujun; Yang, Jinha; Wei, Ying; Sakharkar, Meena Kishore; Yang, Jian

doi:10.3390/bioengineering6040112

Cited by 22 publications

(11 citation statements)

References 38 publications

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“…Earlier researches have shown the effective cytotoxic activity of many organic and aqueous extracts of S. nigrum against multiple cancer cell lines including −29, HT MCF PC −7 (human breast cancer), −12 (human lung cancer) and - HCT 116 (human colon cancer) and its ethanolic extract from berries exhibited poor cytotoxicity against all the cell lines ( Ikeda et al, 2003 , Lee and Lim, 2003 , Heo et al, 2004 , Butt et al, 2018 , Campisi et al, 2019 , Carvalho et al, 2019 , Emam et al, 2019 , Ling et al, 2019 )…”

Section: Discussionmentioning

confidence: 99%

“…The antiproliferative activities of the crude organic solvent-based extracts of S. nigrum and isolated compounds were evaluated on tumor cell lines of liver (HepG2), ( Fekry et al, 2019 ) colon (HT29 and HCT-116), breast (MCF-7), ( Churiyah et al, 2020 ) and cervical (U14 and HeLa) ( Chothiphirat et al, 2019 , Moglad, 2019 ). Cytotoxic effects of a number of glycoalkaloids have also been considered on several cancer cell lines such as HepG2 ( Ahmad et al, 2017 , Ling et al, 2019 , Li et al, 2021 )…”

Section: Discussionmentioning

confidence: 99%

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In vitro cytotoxic potential of Solanum nigrum against human cancer cell lines

Nawaz

Jamal

Arif

et al. 2021

Saudi Journal of Biological Sciences

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Plants have natural products which use to possess antiproliferative potential against many cancers. In the present study, six isolated fractions (ethyl acetate, petroleum ether, chloroform, n-butanol, ethanol and aqueous) from Solanum nigrum were evaluated for their cytotoxic effect on different cell lines. Hepatic carcinoma cell line (HepG2), cervical cancer cell line (HeLa) and baby hamster kidney (BHK) used as normal non-cancerous cells were evaluated for cytotoxicity against isolated fractions. Cell viability assay was performed to evaluate the cytotoxicity of all fractions on different cell lines followed by the lactate dehydrogenase and vascular endothelial growth factor assays of most active fraction among all screened for cytotoxic analysis. HPLC analysis of most active fractions against cytotoxicity was performed to check the biological activity of compounds. Results displayed the potent cytotoxic activity of ethyl acetate fraction of S. nigrum against HepG2 cells with IC 50 value of 7.89 μg/ml. Other fractions exhibited potent anticancer activity against HepG2 cells followed by HeLa cells. Fractions in our study showed no cytotoxicity in BHK cells. Cytotoxic activity observed in our current study exposed high antiproliferative potential and activity of ethyl acetate fraction against HepG2 cells. The results demonstrated that S. nigrum fractions exhibited anticancer activity against hepatic and cervical cancer cell lines with non-toxic effect in normal cells. These results reveal significant potential of S. nigrum for the therapeutic of cancers across the globe in future.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

In vitro cytotoxic potential of Solanum nigrum against human cancer cell lines

Nawaz

Jamal

Arif

et al. 2021

Saudi Journal of Biological Sciences

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“…Furthermore, 1% and 2% SNWE prominently reduced hepatic carcinogenesis to 40% and 20% and significantly increased the survival rate to 90% and 100%, respectively, in AAF/NaNO 2 -induced hepatoma rats ( Hsu et al, 2009 ). SNWE also caused 43% cytotoxicity, inhibited migration, and suppressed the activities of hexokinase and pyruvate on the of human breast cancer cell line (MCF-7) by about 30% and 40% at a concentration of 10 g/L, respectively ( Ling et al, 2019 ).…”

Section: Pharmacological Activitiesmentioning

confidence: 99%

Solanum nigrum Linn.: An Insight into Current Research on Traditional Uses, Phytochemistry, and Pharmacology

Chen¹,

Dai²,

Liu³

et al. 2022

Front. Pharmacol.

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Solanum nigrum Linn., is a common edible medicinal herb of the Solanaceae family which is native to Southeast Asia and is now widely distributed in temperate to tropical regions of Europe, Asia, and America. Traditionally, it has been used to treat various cancers, acute nephritis, urethritis, leucorrhea, sore throat, toothache, dermatitis, eczema, carbuncles, and furuncles. Up to now, 188 chemical constituents have been identified from S. nigrum. Among them, steroidal saponins, alkaloids, phenols, and polysaccharides are the major bioactive constituents. Investigations of pharmacological activities of S. nigrum revealed that this edible medicinal herb exhibits a wide range of therapeutic potential, including antitumor, anti-inflammatory, antioxidant, antibacterial, and neuroprotective activities both in vivo and in vitro. This article presents a comprehensive and systematic overview of the botanical, traditional uses, phytochemical compositions, pharmacological properties, clinical trials, and toxicity of S. nigrum to provide the latest information for further exploitation and applications of S. nigrum in functional foods and medicines.

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“…There are more than 20 species forming the Solanum nigrum L. complex species system, of which four species are mainly distributed in China, namely; Lobelia, Lobelia olivacea, Lobelia yellow-fruited and Lobelia red-fruited ( 22 ). Solanum nigrum L. exhibits anti-tumor activities, particularly against breast and bladder cancers ( 23 , 24 ). It has also been shown to be effective in the treatment of colitis ( 25 ), inhibition of liver fibrosis, and exhibits anti-hepatitis virus ( 26 ), hepatoprotective ( 27 ), antipyretic and analgesic ( 28 ), as well as antihypertensive effects.…”

Section: Introductionmentioning

confidence: 99%

Solasonine Inhibits Pancreatic Cancer Progression With Involvement of Ferroptosis Induction

Liang

Han

et al. 2022

Front. Oncol.

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Pancreatic cancer is a highly fatal malignant tumor of the digestive system. It is characterized by early metastasis and high mortality rates. Solasonine, a steroidal alkaloid, is derived from Solanum nigrum L., a natural herb. Solasonine is associated with excellent anti-tumor effects, however, its effects on pancreatic cancer have not been fully established. Pancreatic cancer cells (PANC-1 and CFPAC-1) were used to verify the in vitro and in vivo effects of solasonine. Metabolomics were used to evaluate its underlying mechanisms. Solasonine promoted PANC-1 and CFPAC-1 cell apoptosis while inhibiting their proliferation, migration and invasion. Mouse xenograft models and metastasis models of ANC-1 and CFPAC-1 confirmed that solasonine blocked tumor formation and metastasis. Metabolomics confirmed the effects of solasonine on glutathione metabolism and SLC7A11-mediated ferroptosis. Furthermore, Co-Immunoprecipitation and Duolink®in situ PLA confirmed that OTUB1, a deubiquitylating enzyme, interacted with SLC7A11 and solasonine to enhance ubiquitinated degradation of SLC7A11 in PANC-1 and CFPAC-1 cells. Besides, molecular docking confirmed that solasonine directly bound TFAP2A and suppressed its protein levels. Bioinformatics and luciferase assays revealed that TFAP2A binds the OTUB1 promoter region, thereby promoting its transcription. In summary, solasonine inhibits the TFAP2A/OTUB1 SLC7A11 axis to activate ferroptosis and suppress pancreatic cancer cell progression.

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Probing the Antitumor Mechanism of Solanum nigrum L. Aqueous Extract against Human Breast Cancer MCF7 Cells

Cited by 22 publications

References 38 publications

In vitro cytotoxic potential of Solanum nigrum against human cancer cell lines

In vitro cytotoxic potential of Solanum nigrum against human cancer cell lines

Solanum nigrum Linn.: An Insight into Current Research on Traditional Uses, Phytochemistry, and Pharmacology

Solasonine Inhibits Pancreatic Cancer Progression With Involvement of Ferroptosis Induction

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