Prion inactivation using a new gaseous hydrogen peroxide sterilisation process

Fichet, Guillaume; Antloga, Kathy; Comoy, Emmanuel; Deslys, Jean‐Philippe; McDonnell, Gerald

doi:10.1016/j.jhin.2007.08.020

Cited by 92 publications

(73 citation statements)

References 19 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The decontamination methods usually involve high temperatures and/or high concentrations of sodium hydroxide or bleach, which may not be appropriate for sensitive medical instruments. Alternative methods have been proposed, including Fenton reaction (23), gaseous hydrogen peroxide (72), enzymatic treatment (73), and other organic chemical treatment (74). These methods require either long reaction times (i.e., Ͼ30 min to several hours) or pretreatment and/or sequential treatment to obtain optimum inactivation levels (i.e., Ͼ5 log 10 ).…”

Section: Discussionmentioning

confidence: 99%

Kinetics of Ozone Inactivation of Infectious Prion Protein

Ding

Neumann

Price

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

eThe kinetics of ozone inactivation of infectious prion protein (PrP Sc , scrapie 263K) was investigated in ozone-demand-free phosphate-buffered saline (PBS). Diluted infectious brain homogenates (IBH) (0.01%) were exposed to a predetermined ozone dose (10.8 ؎ 2.0 mg/liter) at three pHs (pH 4.4, 6.0, and 8.0) and two temperatures (4°C and 20°C). The inactivation of PrP Sc was quantified by determining the in vitro destruction of PrP Sc templating properties using the protein misfolding cyclic amplification (PMCA) assay and bioassay, which were shown to correlate well. The inactivation kinetics were characterized by both Chick-Watson (CW) and efficiency factor Hom (EFH) models. It was found that the EFH model fit the experimental data more appropriately. The efficacy of ozone inactivation of PrP Sc was both pH and temperature dependent. Based on the EFH model, CT (disinfectant concentration multiplied by contact time) values were determined for 2-log 10 , 3-log 10 , and 4-log 10 inactivation at the conditions under which they were achieved. Our results indicated that ozone is effective for prion inactivation in ozone-demand-free water and may be applied for the inactivation of infectious prion in prion-contaminated water and wastewater.

show abstract

Section: Discussionmentioning

confidence: 99%

Kinetics of Ozone Inactivation of Infectious Prion Protein

Ding

Neumann

Price

et al. 2013

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…Hydrogen peroxide liquid showed a degree of protein clumping and full resistance to protease degradation. The use of gaseous peroxide in a standard low-temperature sterilization process may present a useful method for prion inactivation [24].…”

Section: Antimicrobial Action Of Reactive Oxygen Species and Ozone (Omentioning

confidence: 99%

Untitled

2017

OAJTMR

View full text Add to dashboard Cite

“…Some protein often remains adhered to washed medical tools, and the irregularly shaped prion protein (PrPsc) has recently become problematic in this regard in the medical care and food industries [8][9][10][11][12][13][14]. PrPsc is believed to be a structurally deformed version of the properly shaped prion (PrPc) protein, and PrPsc accumulates in the brain and causes diseases.…”

Section: Introductionmentioning

confidence: 99%

Decomposition of Proteins Using a Microwave Air Plasma Sterilizer

Itarashiki

Hayashi

Yonesu

2016

Trans. Mat. Res. Soc. Japan

View full text Add to dashboard Cite

The decomposition of proteins on a surface was investigated using a low-temperature microwave plasma torch sterilization method. Albumin and keratin, used as model proteins, were irradiated with reactive species produced by an air microwave discharge plasma and their decomposition rates were approximately 0.1 mg/cm 2 per 30 min. All chemical bonds in the proteins, including those involved in forming secondary structures, are decomposed by the reactive species. These findings suggest that the microwave plasma torch method could be useful for removing prion proteins from the surfaces of medical devices.

show abstract

Prion inactivation using a new gaseous hydrogen peroxide sterilisation process

Cited by 92 publications

References 19 publications

Kinetics of Ozone Inactivation of Infectious Prion Protein

Kinetics of Ozone Inactivation of Infectious Prion Protein

Untitled

Decomposition of Proteins Using a Microwave Air Plasma Sterilizer

Contact Info

Product

Resources

About