PRIASE 2021 guidelines for reporting animal studies in Endodontology: a consensus‐based development

Nagendrababu, Venkateshbabu; Murray, Peter E.; Ordinola‐Zapata, Ronald; Peters, Ove A.; Rôças, Isabela N.; Siqueira, José F.; Priya, Ekta; Jayaraman, Jayakumar; Pulikkotil, Shaju Jacob; Camilleri, Josette; Boutsioukis, C.; Rossi‐Fedele, Giampiero; Dummer, P. M. H.

doi:10.1111/iej.13477

Cited by 103 publications

(87 citation statements)

References 25 publications

(52 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This animal study was reported according to the PRIASE 2021 Guidelines (Fig. 1; Nagendrababu et al 2021). The general health condition of the rats remained constant throughout the experimental period.…”

Section: Resultsmentioning

confidence: 99%

Histology‐based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers

Tian²,

Xu³

et al. 2021

Int Endodontic J

View full text Add to dashboard Cite

Aim To profile molecular changes in lipopolysaccharide (LPS)-induced experimental pulpitis in a rat model and explore the feasibility of a molecular-based diagnostic strategy for pulpitis. Methodology Seventy-three maxillary incisors of Sprague-Dawley rats were used to establish pulpitis models with LPS. Inflammatory grading was performed in four equal sections of the pulp divided from the injured site to the root apex. An antibody array was used to compare the expression of 67 molecules between control pulp and inflamed pulp 12 and 72 h after LPS application. The levels of differentially expressed molecules in the control and inflamed pulp (collected at 3, 6, 9, 12, 24 and 72 h after LPS treatment) were examined via ELISA, and correlations between inflammatory scores and molecule expression were assessed. The molecule distributions in the pulp were investigated by immunofluorescence staining. Data were analysed with paired t-test, one-way ANOVA, Kruskal-Wallis tests, and Spearman's and Pearson's correlations with significance set at P < 0.05. Results Polymorphonuclearneutrophils were observed in the injured site 3 h after LPS stimulation. Inflammatory infiltration peaked at 12 h and was limited to the injured site with osteodentine deposition at 72 h. Thirteen molecules were significantly differentially expressed between the control and LPS-injured pulp. ELISA validated that tissue inhibitor of metalloproteinase-1 (TIMP-1) expression dramatically peaked at 12 h (compared with other time points, P < 0.05) and returned to baseline at 72 h. The TIMP-1 concentration was strongly correlated with inflammation severity in the apical threequarters of the pulp, and the strongest correlation was found in the lower-middle quarter (r = 0.786, P < 0.001). Immunofluorescence staining revealed that in the apical three-quarters of the pulp, TIMP-1 expression was significantly higher in the 12 h group than in the control and 3, 6, 24 and 72 h groups (P < 0.01). Conclusion This study provides a molecular profile of LPS-induced pulpitis in a rat model. TIMP-1 had a strong positive correlation with the severity of dental pulp inflammation, verifying the feasibility of applying biomarkers to identify specific pathological conditions in pulpitis.

show abstract

Section: Resultsmentioning

confidence: 99%

Histology‐based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers

Tian²,

Xu³

et al. 2021

Int Endodontic J

View full text Add to dashboard Cite

show abstract

“…The following sentence should be included in manuscripts when reports of animal studies followed the PRIASE 2021 guidelines: ‘This animal study was reported according to the PRIASE 2021 Guidelines (Nagendrababu et al . 2021)’. The adoption and endorsement of the PRIASE 2021 guidelines by relevant journals in their ‘Author guidelines’ will ensure the guidelines are used.…”

Section: Discussionmentioning

confidence: 99%

“…It is anticipated these guidelines will be of value to researchers, editors and peer reviewers of scientific journals (Nagendrababu et al . 2021).…”

Section: Introductionmentioning

confidence: 99%

“…The final version of the PRIASE 2021 guidelines consists of a checklist with 43 items under 11 sections and a flowchart (Nagendrababu et al . 2021).…”

Section: Introductionmentioning

confidence: 99%

“…Hence, the PRIASE 2021 guidelines were developed with the objective of improving the standard of manuscripts submitted to journals describing animal studies linked to the specialty of Endodontology. It is anticipated these guidelines will be of value to researchers, editors and peer reviewers of scientific journals (Nagendrababu et al 2021).…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

PRIASE 2021 guidelines for reporting animal studies in Endodontology: explanation and elaboration

et al. 2021

Self Cite

View full text Add to dashboard Cite

Laws and ethics require that before conducting human clinical trials, a new material, device or drug may have to undergo testing in animals in order to minimize health risks to humans, unless suitable supporting grandfather data already exist. The Preferred Reporting Items for Animal Studies in Endodontology (PRIASE) 2021 guidelines were developed exclusively for the specialty of Endodontology by integrating and adapting the ARRIVE (Animals in Research: Reporting In Vivo Experiments) guidelines and the Clinical and Laboratory Images in Publications (CLIP) principles using a validated consensus‐based methodology. Implementation of the PRIASE 2021 guidelines will reduce potential sources of bias and thus improve the quality, accuracy, reproducibility, completeness and transparency of reports describing animal studies in Endodontology. The PRIASE 2021 guidelines consist of a checklist with 11 domains and 43 individual items and a flowchart. The aim of the current document is to provide an explanation for each item in the PRIASE 2021 checklist and flowchart and is supplemented with examples from the literature in order for readers to understand their significance and to provide usage guidance. A link to the PRIASE 2021 explanation and elaboration document and PRIASE 2021 checklist and flowchart is available on the Preferred Reporting Items for study Designs in Endodontology (PRIDE) website (http://pride-endodonticguidelines.org/priase/).

show abstract

Dimensional changes of endodontic sealers—An in vitro model simulating a clinical extrusion scenario during 18 months

Razdan

Benetti

Bakhshandeh

et al. 2023

Clinical & Exp Dental Res

View full text Add to dashboard Cite

Objectives To examine the dimensional changes of endodontic sealers during 18 months using three‐dimensional (3D) surface scanning and subtraction radiography in a novel in vitro sealer‐extrusion model. Material and Methods Fifty endodontically instrumented acrylic teeth were randomly allocated to five groups ( n = 10) filled with Apexit Plus, AH Plus, BioRoot RCS, TubliSeal EWT, and gutta‐percha (control). Freshly mixed sealers were intentionally extruded during obturation. All teeth were immersed in a physiologic solution for up to 18 months. Blinded 3D surface scans (resolution: ~10 μm) and digital radiographs of the teeth were obtained at baseline (immediately after obturation); and then after 1 week, and at 1, 3, and 18 months. For blinded assessment of sealer dimensional change, 3D models and radiographs were superimposed using specific software. Volumetric differences, root mean square (RMS), and area change from subtraction radiography measured at each period within each sealer group were thereafter calculated. Repeated measures analyses were done with Bonferroni adjustment for multiple comparisons; standard errors, p values, and 95% confidence intervals (CI) were reported. Results Analyses of the volumetric data confirmed significant, progressive material loss for Apexit Plus when compared to the other investigated sealers or the control group ( p ≤ 0.02). Immersion period significantly influenced the volumetric dimensional changes of Apexit Plus already after 1 month ( p < 0.01). For TubliSeal EW, the effect of the immersion period on the dimensional changes was noted after immersion for 3 months ( p ≤ 0.02), while for BioRoot RCS this was evident only at 18 months ( p < 0.01). Same trends were noted for the RMS data, whereas progressive dimensional changes using subtraction radiography only revealed significant changes for Apexit Plus ( p = 0.01). Conclusions The largest dimensional changes were shown by Apexit Plus, followed by Tubliseal EWT and BioRoot RCS. AH Plus remained stable throughout 18 months.

show abstract

PRIASE 2021 guidelines for reporting animal studies in Endodontology: a consensus‐based development

Cited by 103 publications

References 25 publications

Histology‐based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers

Histology‐based profile of inflammatory mediators in experimentally induced pulpitis in a rat model: screening for possible biomarkers

PRIASE 2021 guidelines for reporting animal studies in Endodontology: explanation and elaboration

Dimensional changes of endodontic sealers—An in vitro model simulating a clinical extrusion scenario during 18 months

Contact Info

Product

Resources

About