Prevention of posterior capsule opacification by retinoic acid and mitomycin

Abstract

Intraoperative single-dose administration of RA and MMC significantly prevented the development of PCO in rabbit eyes. The optimal biocompatible dosage must be carefully determined by further investigation.

Cytoskeletal drugs prevent posterior capsular opacification in human lens capsule in vitro

“…We have cultured the human lens capsular bag in vitro primarily to follow the dynamics of cell growth and capsular wrinkling after cytoskeletal drug (H-7/LAT-B) or vehicle (BSS/DMSO) treatment. The four-point scale for grading PCO in vitro [30,31] was used to quantify the effectiveness of the drugs.…”

“…Photographic (Nikon Coolpix E8400; 8 Megapixels) images were collected at least once weekly. PCO was graded on a four-point scale at 28 th day of culture: 0-clear, no visible migrating cells on the peripheral and central posterior capsule; 1–mild, migrating/proliferating cells in the periphery only; 2–moderate, proliferating cells on both peripheral and central capsules with sparse wrinkling; 3–dense, apparent LEC growth and severe wrinkle formation on the entire capsule [30, 31]. Treatment of the lens capsular bag with drug or vehicle was masked to the person who maintained the cultures, recorded the microscopic images and carried out the grading.…”

Cytoskeletal drugs prevent posterior capsular opacification in human lens capsule in vitro

“…We have cultured the human lens capsular bag in vitro primarily to follow the dynamics of cell growth and capsular wrinkling after cytoskeletal drug (H-7/LAT-B) or vehicle (BSS/DMSO) treatment. The four-point scale for grading PCO in vitro [30,31] was used to quantify the effectiveness of the drugs.…”

“…Photographic (Nikon Coolpix E8400; 8 Megapixels) images were collected at least once weekly. PCO was graded on a four-point scale at 28 th day of culture: 0-clear, no visible migrating cells on the peripheral and central posterior capsule; 1–mild, migrating/proliferating cells in the periphery only; 2–moderate, proliferating cells on both peripheral and central capsules with sparse wrinkling; 3–dense, apparent LEC growth and severe wrinkle formation on the entire capsule [30, 31]. Treatment of the lens capsular bag with drug or vehicle was masked to the person who maintained the cultures, recorded the microscopic images and carried out the grading.…”

Prevention of Posterior Capsular Opacification through Aldose Reductase Inhibition

“…Although use of antioxidants (such as caffeic and retinoic acids) and corticosteroids has also been suggested,22-24,48 their effectiveness is marred either by toxicity and/or only partial prevention of the PCO. Our results suggest that AR inhibitors block signaling events that lead to both LEC proliferation and differentiation/transdifferentiation indicating that inhibition of AR could be a novel therapeutic approach to prevent PCO.…”

Impact of posterior subcapsular opacification on vision and visual function among subjects undergoing cataract surgery in rural China: Study of Cataract Outcomes and Up-Take of Services (SCOUTS) in the Caring is Hip Project, Report 5

“…Can the long-term follow-up required to detect a high proportion of eventual PCO be delivered sustainably in rural Asia? What will be the role in this setting of prophylactic interventions such as primary capsulotomy with posterior optic buttonholing,34 and various techniques to reduce PCO prevalence, including alternative lens designs13 – 16 and materials,12 17 and pharmacological,18 – 20 radiological,21 and phototherapeutic22 techniques? Given that cataract is the world’s leading cause of blindness, and PCO the most common postoperative complication of cataract surgery,1 more research is needed in this critical area.…”

Cytoskeletal drugs prevent posterior capsular opacification in human lens capsule in vitro

“…We have cultured the human lens capsular bag in vitro primarily to follow the dynamics of cell growth and capsular wrinkling after cytoskeletal drug (H-7/LAT-B) or vehicle (BSS/DMSO) treatment. The four-point scale for grading PCO in vitro [30,31] was used to quantify the effectiveness of the drugs.…”

“…Photographic (Nikon Coolpix E8400; 8 Megapixels) images were collected at least once weekly. PCO was graded on a four-point scale at 28 th day of culture: 0-clear, no visible migrating cells on the peripheral and central posterior capsule; 1–mild, migrating/proliferating cells in the periphery only; 2–moderate, proliferating cells on both peripheral and central capsules with sparse wrinkling; 3–dense, apparent LEC growth and severe wrinkle formation on the entire capsule [30, 31]. Treatment of the lens capsular bag with drug or vehicle was masked to the person who maintained the cultures, recorded the microscopic images and carried out the grading.…”

Prevention of Posterior Capsular Opacification through Aldose Reductase Inhibition

“…Although use of antioxidants (such as caffeic and retinoic acids) and corticosteroids has also been suggested,22-24,48 their effectiveness is marred either by toxicity and/or only partial prevention of the PCO. Our results suggest that AR inhibitors block signaling events that lead to both LEC proliferation and differentiation/transdifferentiation indicating that inhibition of AR could be a novel therapeutic approach to prevent PCO.…”

Impact of posterior subcapsular opacification on vision and visual function among subjects undergoing cataract surgery in rural China: Study of Cataract Outcomes and Up-Take of Services (SCOUTS) in the Caring is Hip Project, Report 5

“…Can the long-term follow-up required to detect a high proportion of eventual PCO be delivered sustainably in rural Asia? What will be the role in this setting of prophylactic interventions such as primary capsulotomy with posterior optic buttonholing,34 and various techniques to reduce PCO prevalence, including alternative lens designs13 – 16 and materials,12 17 and pharmacological,18 – 20 radiological,21 and phototherapeutic22 techniques? Given that cataract is the world’s leading cause of blindness, and PCO the most common postoperative complication of cataract surgery,1 more research is needed in this critical area.…”

Cytoskeletal drugs prevent posterior capsular opacification in human lens capsule in vitro

“…We have cultured the human lens capsular bag in vitro primarily to follow the dynamics of cell growth and capsular wrinkling after cytoskeletal drug (H-7/LAT-B) or vehicle (BSS/DMSO) treatment. The four-point scale for grading PCO in vitro [30,31] was used to quantify the effectiveness of the drugs.…”

“…Photographic (Nikon Coolpix E8400; 8 Megapixels) images were collected at least once weekly. PCO was graded on a four-point scale at 28 th day of culture: 0-clear, no visible migrating cells on the peripheral and central posterior capsule; 1–mild, migrating/proliferating cells in the periphery only; 2–moderate, proliferating cells on both peripheral and central capsules with sparse wrinkling; 3–dense, apparent LEC growth and severe wrinkle formation on the entire capsule [30, 31]. Treatment of the lens capsular bag with drug or vehicle was masked to the person who maintained the cultures, recorded the microscopic images and carried out the grading.…”

Prevention of Posterior Capsular Opacification through Aldose Reductase Inhibition

“…Although use of antioxidants (such as caffeic and retinoic acids) and corticosteroids has also been suggested,22-24,48 their effectiveness is marred either by toxicity and/or only partial prevention of the PCO. Our results suggest that AR inhibitors block signaling events that lead to both LEC proliferation and differentiation/transdifferentiation indicating that inhibition of AR could be a novel therapeutic approach to prevent PCO.…”

Impact of posterior subcapsular opacification on vision and visual function among subjects undergoing cataract surgery in rural China: Study of Cataract Outcomes and Up-Take of Services (SCOUTS) in the Caring is Hip Project, Report 5

“…Can the long-term follow-up required to detect a high proportion of eventual PCO be delivered sustainably in rural Asia? What will be the role in this setting of prophylactic interventions such as primary capsulotomy with posterior optic buttonholing,34 and various techniques to reduce PCO prevalence, including alternative lens designs13 – 16 and materials,12 17 and pharmacological,18 – 20 radiological,21 and phototherapeutic22 techniques? Given that cataract is the world’s leading cause of blindness, and PCO the most common postoperative complication of cataract surgery,1 more research is needed in this critical area.…”