Prevention of acetaminophen-induced hepatotoxicity by dimethyl sulfoxide

Effects of Dimethylsulfoxide on Metabolism and Toxicity of Acetaminophen in Mice

“…p-Nitrophenol hydroxylase and p-nitroanisole demethylase activities, in which CYP2E1 plays a major role, 23,32) were increased from 2 h following the treatment. This is in accordance with earlier studies showing an increase in the hepatic aniline hydroxylase activity, 7) mRNA levels, 31) and expression of CYP2E1 30) by DMSO administration to rodents. In contrast, DMSO pretreatment decreased aminopyrine N-demethylase and erythromycin N-demethylase activities, in which CYP1A2 and/or 3A are involved.…”

Effects of Dimethylsulfoxide on Metabolism and Toxicity of Acetaminophen in Mice

“…p-Nitrophenol hydroxylase and p-nitroanisole demethylase activities, in which CYP2E1 plays a major role, 23,32) were increased from 2 h following the treatment. This is in accordance with earlier studies showing an increase in the hepatic aniline hydroxylase activity, 7) mRNA levels, 31) and expression of CYP2E1 30) by DMSO administration to rodents. In contrast, DMSO pretreatment decreased aminopyrine N-demethylase and erythromycin N-demethylase activities, in which CYP1A2 and/or 3A are involved.…”

Evaluation of Three-Dimensional Cultured HepG2 Cells in a Nano Culture Plate System: an In Vitro Human Model of Acetaminophen Hepatotoxicity

“…DMSO has been commonly used in biological studies as an inert solvent to dissolve drugs with low water solubility. However, DMSO has been known to attenuate liver injury induced by APAP overdose in rodents, and often the facts confound evaluation of the protective effects of compounds with poor water solubility against APAP-induced liver injury (47,48). In this study, we also confirmed that the treatment with 5% DMSO attenuated the increase in serum alanine aminotransferase levels and hepatocellular necrosis induced by APAP (400 mg/kg, i.p.)…”

Lysosomal Instability and CathepsinBRelease during Acetaminophen Hepatotoxicity

“…This is similar to the previous experience with the JNK inhibitor SP600125 where the administration of a significant amount of DMSO cannot be avoided . However, DMSO is a potent inhibitor of P450 and consequently protects against APAP toxicity , even at the low doses administered in these inhibitor studies . To overcome this problem, the DMSO‐soluble inhibitor was injected after APAP, and the dose of APAP was increased to 600 mg/kg, which results in liver injury despite the presence of DMSO .…”

Effects of Dimethylsulfoxide on Metabolism and Toxicity of Acetaminophen in Mice

“…p-Nitrophenol hydroxylase and p-nitroanisole demethylase activities, in which CYP2E1 plays a major role, 23,32) were increased from 2 h following the treatment. This is in accordance with earlier studies showing an increase in the hepatic aniline hydroxylase activity, 7) mRNA levels, 31) and expression of CYP2E1 30) by DMSO administration to rodents. In contrast, DMSO pretreatment decreased aminopyrine N-demethylase and erythromycin N-demethylase activities, in which CYP1A2 and/or 3A are involved.…”

Evaluation of Three-Dimensional Cultured HepG2 Cells in a Nano Culture Plate System: an In Vitro Human Model of Acetaminophen Hepatotoxicity

“…DMSO has been commonly used in biological studies as an inert solvent to dissolve drugs with low water solubility. However, DMSO has been known to attenuate liver injury induced by APAP overdose in rodents, and often the facts confound evaluation of the protective effects of compounds with poor water solubility against APAP-induced liver injury (47,48). In this study, we also confirmed that the treatment with 5% DMSO attenuated the increase in serum alanine aminotransferase levels and hepatocellular necrosis induced by APAP (400 mg/kg, i.p.)…”

Lysosomal Instability and CathepsinBRelease during Acetaminophen Hepatotoxicity

“…This is similar to the previous experience with the JNK inhibitor SP600125 where the administration of a significant amount of DMSO cannot be avoided . However, DMSO is a potent inhibitor of P450 and consequently protects against APAP toxicity , even at the low doses administered in these inhibitor studies . To overcome this problem, the DMSO‐soluble inhibitor was injected after APAP, and the dose of APAP was increased to 600 mg/kg, which results in liver injury despite the presence of DMSO .…”

Effects of Dimethylsulfoxide on Metabolism and Toxicity of Acetaminophen in Mice

“…p-Nitrophenol hydroxylase and p-nitroanisole demethylase activities, in which CYP2E1 plays a major role, 23,32) were increased from 2 h following the treatment. This is in accordance with earlier studies showing an increase in the hepatic aniline hydroxylase activity, 7) mRNA levels, 31) and expression of CYP2E1 30) by DMSO administration to rodents. In contrast, DMSO pretreatment decreased aminopyrine N-demethylase and erythromycin N-demethylase activities, in which CYP1A2 and/or 3A are involved.…”

Evaluation of Three-Dimensional Cultured HepG2 Cells in a Nano Culture Plate System: an In Vitro Human Model of Acetaminophen Hepatotoxicity

“…DMSO has been commonly used in biological studies as an inert solvent to dissolve drugs with low water solubility. However, DMSO has been known to attenuate liver injury induced by APAP overdose in rodents, and often the facts confound evaluation of the protective effects of compounds with poor water solubility against APAP-induced liver injury (47,48). In this study, we also confirmed that the treatment with 5% DMSO attenuated the increase in serum alanine aminotransferase levels and hepatocellular necrosis induced by APAP (400 mg/kg, i.p.)…”

Lysosomal Instability and CathepsinBRelease during Acetaminophen Hepatotoxicity

“…This is similar to the previous experience with the JNK inhibitor SP600125 where the administration of a significant amount of DMSO cannot be avoided . However, DMSO is a potent inhibitor of P450 and consequently protects against APAP toxicity , even at the low doses administered in these inhibitor studies . To overcome this problem, the DMSO‐soluble inhibitor was injected after APAP, and the dose of APAP was increased to 600 mg/kg, which results in liver injury despite the presence of DMSO .…”