Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará

Silva, Jenevaldo B.; Lopes, Cinthia T.A.; Pinheiro, Cleyton Prado; Lima, Danilo H.S.; Silva, Roberto S.L.; Fonseca, Adivaldo Henrique da; Araújo, Flábio R.

doi:10.1590/s0100-736x2013000700003

Cited by 8 publications

(6 citation statements)

References 14 publications

(17 reference statements)

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“…Desta forma, como também ocorre na Ilha de Marajó-PA (SILVA et al, 2013a;SILVA et al, 2014), os bubalinos poderão atuar como reservatórios dos agentes da TPB, representando um risco para o rebanho onde búfalos e bovinos são mantidos juntos (SILVA et al, 2013b). A prevalência de animais soropositivos para agentes transmitidos por carrapatos sugere um estudo mais detalhado da epidemiologia da TPB, em bovinos e bubalinos, na região.…”

Section: Resultsunclassified

Ocorrência sorológica de Babesia bovis, Babesia bigemina e Anaplasma marginale em bovinos e bubalinos no estado do Pará, Brasil

et al. 2014

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ResumoDentre as principais enfermidades que acometem bovinos e bubalinos, destacam-se as doenças transmitidas por carrapatos. As babesioses bovinas, causadas por Babesia bigemina e Babesia bovis, e a anaplasmose, causada pela rickettsia Anaplasma marginale, são doenças de grande importância econômica para a bovinocultura. O objetivo deste trabalho foi comparar a ocorrência sorológica para os agentes da Tristeza Parasitária em bovinos e bubalinos na mesorregião sudeste do estado do Pará, região norte do Brasil. AbstractAmong the major diseases that affect cattle and water buffaloes, the tick-borne diseases are detaches. Bovine babesiosis caused by Babesia bigemina and Babesia bovis, and anaplasmosis, caused by Anaplasma marginale rickettsia, are of great economic importance to cattle diseases. The aim of this study was to compare the serologic occurrence for the agents of tick fever in cattle and water buffaloes

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Section: Resultsunclassified

Ocorrência sorológica de Babesia bovis, Babesia bigemina e Anaplasma marginale em bovinos e bubalinos no estado do Pará, Brasil

et al. 2014

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“…Previously, a percentage of 40% of calves showed A. marginale-PCR positive results in the first day after birth in a study done in Rio de Janeiro (SILVA et al, 2013). On the other hand, 13% of calves sampled in the state of Minas Gerais showed A. marginale-PCR positive results at the time of birth (POHL et al, 2013).…”

Section: Transplacental Transmissionmentioning

confidence: 95%

“…Initially, the detection of the presence of A. marginale DNA was made using 1.0µl each (20pmol) of primers targeting part of msp-5 gene (a fragment of approximately 458 bp), namely Amar msp-5 eF (5' GCA TAG CCT CCG CGT CTT TC 3') and Amar msp5 eR (5' TCC TCG CCT TGG CCC TCA GA 3'), previously described by de Torioni De Echaide et al (1998) and optimized by Singh et al (2012). PCR amplifications were performed at 94ºC for 1 min followed by 34 repetitive cycles of 94ºC for 30 min, 58ºC for 30 min, and 72ºC for 1 min, followed by a final extension at 72ºC for 10 min (SINGH et al, 2012).…”

Section: Semi-nested Pcrmentioning

confidence: 99%

“…PCR amplifications were performed at 94ºC for 1 min followed by 34 repetitive cycles of 94ºC for 30 min, 58ºC for 30 min, and 72ºC for 1 min, followed by a final extension at 72ºC for 10 min (SINGH et al, 2012). Each sample of extracted DNA was used as a template in 25 µL reaction mixtures containing 10X PCR buffer, 1.5 mM MgCl2, 0.5 mM desoxynucleotide triphosphate (dNTPs) mixture, 1.0 UTaq DNA Polymerase (Invitrogen, Carlsbad, California, USA).…”

Section: Semi-nested Pcrmentioning

confidence: 99%

“…Each sample of extracted DNA was used as a template in 25 µL reaction mixtures containing 10X PCR buffer, 1.5 mM MgCl2, 0.5 mM desoxynucleotide triphosphate (dNTPs) mixture, 1.0 UTaq DNA Polymerase (Invitrogen, Carlsbad, California, USA). Amplified products from the first reaction were used in another using primers Amar msp5 eR (5' TCC TCG CCT TGG CCC TCA GA 3') and Amar msp5 iF (5' TAC ACG TGC CCT ACC GAG TTA 3') to a fragment of approximately 345 bp (SINGH et al, 2012). The cycling conditions were the same for the PCR with primers Amar msp-5 eF and Amar msp5 eR.…”

Section: Semi-nested Pcrmentioning

confidence: 99%

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Longitudinal study of risk factors for anaplasmosis and transplacental transmission in herd cattle

2014

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Anaplasma marginale, bacteria that occurs in tropical and subtropical areas throughout the world, is responsible for causing a disease that is one of the most constraint to cattle production in many countries. The present work aimed to investigate potential risk factors for anaplasmosis and the occurrence of transplacental transmission by molecular and serological assays in cattle. A total of 22 calves were randomly sampled and monitored during four years; the presence of infection/exposure to A. marginale was assessed by a semi-nested PCR assay targeting msp-5 gene of A. marginale, indirect ELISA for detection of IgG antibodies to A. marginale and blood smears. The A. marginale infection prevalence data were analyzed in relation to risk factors, using chi-squared, odds ratio and multiple logistic regressions. DNA amplification results revealed a frequency of infection with A. marginale of 41% (9/22) in the newborn calves before colostrum suckling. However, only 14% (3/22) of newborn calves were seropositive for A. marginale. All calves were positive to A. marginale by PCR and microscopic examination of blood smears before the two and fourth month of age, respectively. The main risk factors associated with seroprevalence were breed (OR=36.2), tick infestation (OR=3.44) and stocking density (OR=3.28). The results indicated that exposure of cattle to A. marginale was common in dairy herds and endemic instability situation probably is due to inadequate antibody production in cows or genetic variability of pathogen. This study still demonstrated that in addition to transmission of A. marginale by ticks and flies, the transplacental transmission is very important and should be the target of disease control programs in Brazil. Key words: Anaplasma marginale, blood smear, ELISA, PCR ResumoAnaplasma marginale, bactéria que ocorre em regiões tropicais e subtropicais do mundo, é responsável por causar uma das doenças que mais restringem a criação de bovinos em diversos países. O presente trabalho teve como objetivo investigar potenciais fatores de risco para a anaplasmose e a ocorrência de transmissão transplacentária por ensaios moleculares e sorológicos em bovinos. (OR = 3,28). Os resultados indicaram que a exposição de gado para A. marginale era comum nos rebanhos leiteiros e a situação de instabilidade endêmica provavelmente se faz devido à produção inadequada de anticorpos em vacas ou a variabilidade genética do patógeno. Este estudo demonstra ainda que, além da transmissão de A. marginale por carrapatos e moscas, a transmissão transplacentária é muito importante e deve ser alvo de programas de controle da doença no Brasil.

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Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR

Vieira

Canever

Cardozo

et al. 2019

Parasite Epidemiology and Control

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This study aimed to determine the prevalence of three common hemoparasites (Anaplasma marginale, Babesia bovis and Babesia bigemina) in cattle from 16 counties in the Campos de Lages region, Santa Catarina state, Brazil, and the factors affecting disease occurrence. The study population consisted of 257 clinically healthy animals from 21 rural farms. Bovine blood samples were collected by jugular venipuncture. DNA was extracted from whole blood by the phenol/ chloroform method. Genomic DNA extracted from blood samples was subjected to Multiplex PCR for screening of B. bovis, B. bigemina, and A. marginale using specific primers. Prevalences of A. marginale, B. bigemina, and B. bovis were 27%, 16%, and 29%, respectively. Mixed infection was observed in 17.5% of samples. The most frequent was Babesia bovis and Babesia bigemina in 6.62% of samples. A. marginale infection rates were statistically correlated with age groups of cattle. The infections detected in the study population were considered to be subclinical, based on the presence pathogen DNA and absence of clinical symptoms. Seasonality of the pathogens resulted in various degrees of infection, related to the age of the animals and the season. The Campos de Lages region is characterized by enzootic instability for these pathogens because of its climatic and geographic features.

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Prevalência sorológica e molecular de Babesia bovis e Babesia bigemina em búfalos (Bubalus bubalis) na Ilha de Marajó, Pará

Abstract: Pesq. Vet. Bras. 33 (7)

Cited by 8 publications

References 14 publications

Ocorrência sorológica de Babesia bovis, Babesia bigemina e Anaplasma marginale em bovinos e bubalinos no estado do Pará, Brasil

Ocorrência sorológica de Babesia bovis, Babesia bigemina e Anaplasma marginale em bovinos e bubalinos no estado do Pará, Brasil

Longitudinal study of risk factors for anaplasmosis and transplacental transmission in herd cattle

Prevalence of Anaplasma marginale, Babesia bovis, and Babesia bigemina in cattle in the Campos de Lages region, Santa Catarina state, Brazil, estimated by multiplex-PCR

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