Prevalence, virulence characterization, and genetic relatedness of Listeria monocytogenes isolated from chicken retail points and poultry slaughterhouses in Turkey

Coban, Aysen; Pennone, Vincenzo; Sudağıdan, Mert; Molva, Celenk; Jordan, Kieran; Aydın, Ali

doi:10.1007/s42770-019-00133-y

Cited by 12 publications

(8 citation statements)

References 72 publications

(96 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The authors serogrouped 103 isolates originating from poultry samples from the retail markets and slaughterhouses in Turkey. However, the of L. monocytogenes strains (78 samples -75.7 %) were characterised as the IIa serogroup (which is in agreement with our findings), followed by the IIc serogroup (15 samples -14.5 %) and the IIb group (6 samples -5.8 %) [7].…”

Section: Resultssupporting

confidence: 92%

“…That study demonstrated that the 1/2a serotype was the most commonly prevalent in food products, however, the authors did not detect any isolate which belonged to the 4b, 4d or 4e serovars, whereas in our case, the IVb serogroup was the second most common. Similarly to findings of Wang et al [22], according to Coban et al [7], who used the protocol of Doumith et al [9], the IVb serogroup was the least numerous (1 sample -1.0 %) in their study. The authors serogrouped 103 isolates originating from poultry samples from the retail markets and slaughterhouses in Turkey.…”

Section: Resultssupporting

confidence: 73%

See 1 more Smart Citation

Genoserotyping of Listeria Monocytogenes Strains Originating From Meat Products and Meat Processing Environments

Kawacka¹,

Olejnik-Schmidt²

2022

zntj

View full text Add to dashboard Cite

Background. Listeria monocytogenes is a foodborne human pathogen and a causative factor of listeriosis, which is an illness with a high mortality rate. Serotyping is a method for differentiating L. monocytogenes isolates based on unique combinations of somatic (O) and flagellar (H) antigens on the surface of their cells. Standard serotyping involves agglutination methods, which require using antisera. However, there are also genoserotyping methods which allow to categorise L. monocytogenes isolates into particular groups of serotypes (referred to as serogroups) based on genetic analyses. Differentiating L. monocytogenes isolates is an important issue in terms of food safety, surveillance and traceability of contamination sources. In this work, we present results of the genoserotyping of 153 L. monocytogenes isolates originating from meat products and meat processing environments at Polish processing plants. Two protocols were used for genoserotyping analyses: the first one allows to differentiate between four most common serotypes (1/2a, 1/2b, 1/2c and 4b) and the second one allows to distinguish hipervirulent serovar 4h from other serotypes. Results and conclusion. Results achieved using both methods were consistent and all isolates were categorised into corresponding serogroups within the two methodologies. Most of the isolates (73.9 %) were characterised as members of the IIa serogroup (representing the 1/2a, 3a serovars). The IVb (4b, 4d, 4e) serogroup was the second most common (and comprised 18.3 % of isolates), followed by IIb (1/2b, 3b, 7) and IIc (1/2c, 3c), however, the last two groups were equally numerous (and each of them comprised 3.9 % of all isolates). None of the collected isolates belonged to the serogroup representing the 4a, 4c, 4ab and 4h serotypes.

show abstract

Section: Resultssupporting

confidence: 92%

Section: Resultssupporting

confidence: 73%

Genoserotyping of Listeria Monocytogenes Strains Originating From Meat Products and Meat Processing Environments

Kawacka¹,

Olejnik-Schmidt²

2022

zntj

View full text Add to dashboard Cite

show abstract

“…In addition, 8 of the 12 samples contained the actA and hlyA genes. It has been reported that differences in the prevalence of virulence genes may be related to the use of several PCR target fragments within the genes (Coban et al 2019).…”

Section: Discussionmentioning

confidence: 99%

Virulence properties of Listeria monocytogenes isolated from meat and meat contact surfaces in a slaughterhouse

Tasci,

Sudagidan,

Yavuz

et al. 2024

Polish Journal of Veterinary Sciences

View full text Add to dashboard Cite

Listeria monocytogenes is a ubiquitous microorganism that is isolated from a variety of sources such as soil, water, decaying vegetation, sewage, animal feeds, silage, farm environments and food-processing environments. This study aimed to determine the prevalence, serogroups, biofilm formation, virulence factor genes, and genetic relationships of L. monocytogenes strains isolated from beef meat and meat contact surfaces obtained from a slaughterhouse in Burdur, Turkey. In this study, a total of 179 beef meat and meat contact surface samples were analyzed for the presence of L. monocytogenes by polymerase chain reaction (PCR). Out of a total of 179 beef meat and meat contact surface samples, 83 (46.37%) were found to be contaminated with L. monocytogenes, with the highest incidence (53.01%) occurring in beef meat. In the present study, most of the isolated strains belonged to serogroups IIB and IVB (lineage I). The L. monocytogenes strain also contained monoA-B, prfA, plcA, plcB, mpl, hlyA, actA, gtcA, dltA, Fri, flaA, InlA, InlC, InlJ, and iap genes. Biofilm formation was not determined in the tested samples at pH 5.5 and different temperatures (4°C, 10°C, 25°C, and 37°C). However, strong biofilm formation was observed in 6.45% (2/31) of the strains at pH 7.0 after 48 h incubation at 37°C, and in 3.22% (1/31) of the strains at pH 7.0 after 48 h incubation at 4°C and 10°C. Pulsed-field gel electrophoresis (PFGE) results showed that L. monocytogenes isolates were clonally related, and cross-contamination was present. In addition, PFGE results also revealed that AscI had more distinguishing power than the ApaI restriction enzyme. These results indicate that L. monocytogenes detected from meat and meat contact surfaces in the slaughterhouse pose a potential risk to public health.

show abstract

“…Very few data are available at the European level on L. monocytogenes CCs and WGS analysis in poultry. Most of the existing studies have used old typing techniques such as serotyping and PFGE [ 5 , 6 , 43 , 44 ].…”

Section: Discussionmentioning

confidence: 99%

“…Contamination of chicken meat and foods prepared using chicken meat can occur at various stages before marketing, including the primary production stage, abattoir, processing plant, and retail stores [ 5 ]. In recent years, numerous studies on the presence of L. monocytogenes in these matrices have been conducted worldwide [ 5 , 6 , 7 , 8 , 9 ]. The consumption of cooked chicken products was previously associated with the transmission of listeriosis and caused several listeriosis outbreaks in different countries [ 10 , 11 , 12 , 13 ].…”

Section: Introductionmentioning

confidence: 99%

The Slaughterhouse as Hotspot of CC1 and CC6 Listeria monocytogenes Strains with Hypervirulent Profiles in an Integrated Poultry Chain of Italy

et al. 2023

View full text Add to dashboard Cite

In Europe, very few studies are available regarding the diversity of Listeria monocytogenes (L. monocytogenes) clonal complexes (CCs) and sequence types (ST) in poultry and on the related typing of isolates using whole genome sequencing (WGS). In this study, we used a WGS approach to type 122 L. monocytogenes strains isolated from chicken neck skin samples collected in two different slaughterhouses of an integrated Italian poultry company. The studied strains were classified into five CCs: CC1-ST1 (21.3%), CC6-ST6 (22.9%), CC9-ST9 (44.2%), CC121-ST121 (10.6%) and CC193-ST193 (0.8%). CC1 and CC6 strains presented a virulence gene profile composed of 60 virulence genes and including the Listeria Pathogenicity Island 3, aut_IVb, gltA and gltB. According to cgMLST and SNPs analysis, long-term persistent clusters belonging to CC1 and CC6 were found in one of the two slaughterhouses. The reasons mediating the persistence of these CCs (up to 20 months) remain to be elucidated, and may involve the presence and the expression of stress response and environmental adaptation genes including heavy metals resistance genes (cadAC, arsBC, CsoR-copA-copZ), multidrug efflux pumps (mrpABCEF, EmrB, mepA, bmrA, bmr3, norm), cold-shock tolerance (cspD) and biofilm-formation determinants (lmo0673, lmo2504, luxS, recO). These findings indicated a serious risk of poultry finished products contamination with hypervirulent L. monocytogenes clones and raised concern for the consumer health. In addition to the AMR genes norB, mprF, lin and fosX, ubiquitous in L. monocytogenes strains, we also identified parC for quinolones, msrA for macrolides and tetA for tetracyclines. Although the phenotypical expression of these AMR genes was not tested, none of them is known to confer resistance to the primary antibiotics used to treat listeriosis The obtained results increase the data on the L. monocytogenes clones circulating in Italy and in particular in the poultry chain.

show abstract

Prevalence, virulence characterization, and genetic relatedness of Listeria monocytogenes isolated from chicken retail points and poultry slaughterhouses in Turkey

Cited by 12 publications

References 72 publications

Genoserotyping of Listeria Monocytogenes Strains Originating From Meat Products and Meat Processing Environments

Genoserotyping of Listeria Monocytogenes Strains Originating From Meat Products and Meat Processing Environments

Virulence properties of Listeria monocytogenes isolated from meat and meat contact surfaces in a slaughterhouse

The Slaughterhouse as Hotspot of CC1 and CC6 Listeria monocytogenes Strains with Hypervirulent Profiles in an Integrated Poultry Chain of Italy

Contact Info

Product

Resources

About