Prevalence of vibrios with special reference to Vibrio parahaemolyticus in farmed penaeid shrimp, Penaeus vannamei (Boone, 1931) from selected districts of Tamil Nadu, India

Abstract

Prevalence of Vibrio spp. with special reference to Vibrio parahaemolyticus (Vp) infection was assessed in Penaeus vannamei farms in Kancheepuram and Thiruvallur districts of Tamil Nadu during the period August 2014 to February 2015. Isolation and identification of bacteria from haemolymph, stomach and hepatopancreas of P. vannamei was done from all the farms for the prevalence of Vp infection. Based on the morphological, physiological and biochemical characterisation, 74 isolates were identified as Vp (35.14%), V. harveyi (21.62%), V. anguillarum (16.22%), V. campbellii (10.81%), V. mimicus (8.11%), V. alginolyticus (5.41%) and Pseudomonas aeruginosa (2.7%). The PCR results showed that there were 26 (35.14%) isolates positive for Vp specific toxR and tlh genes and negative for human pathogenic tdh and trh genes. All isolates were also negative for acute hepatopancreatic necrosis disease (AHPND) specific AP1, AP2, AP3 (pirAvp) and AP4 (pirAvp and pirBvp) genes. The study revealed that there was no AHPND causing Vp strain in farmed P. vannamei from two important coastal districts of Tamil Nadu, India.

<p>Metabolic mechanism of ceftazidime resistance in <em>Vibrio alginolyticus</em></p>

“…Pathogenic Vibrio species cause intestinal disease in humans and systemic disease in fish and shrimp. The most common pathogenic Vibrio strains are Vibrio alginolyticus , V. parahaemolyticus , V. vulnificus , and V. cholera , which cause cholera 1–4. Diseases caused by Vibrio pathogens have been treated or prevented with antibiotic drugs, with tremendous benefit to human health and animal breeding.…”

“…The most common pathogenic Vibrio strains are Vibrio alginolyticus, V. parahaemolyticus, V. vulnificus, and V. cholera, which cause cholera. [1][2][3][4] Diseases caused by Vibrio pathogens have been treated or prevented with antibiotic drugs, with tremendous benefit to human health and animal breeding. However, widespread use, as well as misuse and/or inappropriate use of antibiotic therapy have promoted emergence and rapid spread of antibiotic-resistant species of Vibrio and other pathogens.…”

Quantitative Risk Assessment of Vibrio parahaemolyticus Toxi Infection Associated with the Consumption of Roasted Shrimp (Penaeus monodon)

“…e mixture was incubated for 6 h at 37 °C. After the enrichment incubation, the Vibrio concentration was evaluated using the standard counting method on TCBS agar [18,19]. (1) was used to deduce the microbial concentration before enrichment (No).…”

“…is was done by reviewing different papers on V. parahaemolyticus pathogenicity [1,18,19,23,24]. Based on the principle of precaution, the incapacity of the Cameroonian food safety system to selectively differentiate haemolytic and nonhemolytic carrier strains, and the fact that nonhemolytic carrier strains have been observed to cause severe illnesses, the hazard in this risk assessment was focused on the total number of V. parahaemolyticus in raw shrimp (P. monodon) and after roasting.…”

Comparative immunogenicity of outer membrane protein K and whole‐cell antigens of Vibrio parahaemolyticus for diagnosis

“…Out of a total of 142 species, 12 Vibrio species are known human pathogens (Raja et al . 2017a,2017b), of which V. cholerae , V. parahaemolyticus , and V. vulnificus are the most frequently reported (Bonnin‐Jusserand et al . 2019).…”