Prevalence of microbial biofilms on selected fresh produce and household surfaces

Rayner, Joanna; Veeh, Richard Harold; Flood, Janice Elizabeth Desi

doi:10.1016/j.ijfoodmicro.2004.01.019

Cited by 80 publications

(51 citation statements)

References 27 publications

(31 reference statements)

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“…Alcian blue stains the acidic polysaccharides often present in the biofilm matrix (extracellular polymeric substances, or EPS) (24). An aqueous solution of 0.1% alcian blue 8GX (Sigma, Kanagawa, Japan) was directly applied to the sections to stain the EPS.…”

Section: Microscopymentioning

confidence: 99%

“…These color changes correspond to the growth of microbes attached to the bare reed and the gradual formation of a micro-scale colony along with formation of the biofilm; microbial cells buried in the extracellular polymeric substances (EPS), produced by the microorganisms attached to the reed, were well stained with alcian blue. Acidic polymers are usually stained well with this dye (24,25). The small colonies would eventually merge during continued microbial growth and EPS production, which along with the formation of the biofilm from microcolonies patchily distributed on the reed led to coverage of the surface.…”

Section: Biofilm Stainingmentioning

confidence: 99%

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Analysis of How a Biofilm Forms on the Surface of the Aquatic Macrophyte Phragmites australis

et al. 2009

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The process by which a biofilm forms on the surface of the aquatic macrophyte Phragmites australis was investigated over a period of about two months (from mid-May to late-July, 2008) in Lake Biwa. The biofilm formed relatively quickly, its wet weight per unit area after seven day being that of a mature biofilm. This speed can be attributed to the many active bacteria in the early stage of its formation and the extracellular polymeric substances (EPS) they produce. The EPS carried electric charges that attracted nutrient ions from surrounding lake water, which, by electrostatic interaction, reached a high concentration as early as day 7 of the formation process. This significantly affected the biofilm community, which differed greatly from that of the lake water even at the beginning of biofilm formation. Brown amorphous compounds (a complex of organic and inorganic substances), covered the biofilm in the second half of its formation process producing a different community structure from that initially. This study revealed a fast and dynamic process of biofilm formation on the reed surface of reed.

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Section: Microscopymentioning

confidence: 99%

Section: Biofilm Stainingmentioning

confidence: 99%

Analysis of How a Biofilm Forms on the Surface of the Aquatic Macrophyte Phragmites australis

et al. 2009

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“…Biofilms have also been found in the domestic environment. For example in dishwashers [5], in showerheads [6] or on household surfaces [7] biofilms have been found and opportunistic pathogens were identified. Washing machines have been proven to be an ideal habitat for microbial communities as well [8].…”

Section: Introductionmentioning

confidence: 99%

Characterization of Microbial Communities in Household Washing Machines

Nix

Frontzek

Bockmühl

2015

Tenside Surfactants Detergents

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Although microbial contaminations of domestic appliances are believed to gain importance, especially due to the trend towards lower cleaning temperatures, little is known about the existing microbial communities inside household washing machines. This study aimed to improve the knowledge of microbial composition of biofilms within household washing machines using a cultural-independent technology. Biofilms inside the detergent drawer and the rubber door seal of household washing machines were analysed using the 454-pyrosequencing method. The microbial community inside washing machines appeared to be highly diverse with Proteobacteria as the main prokaryotic and Basidiomycota and Ascomycota as the main fungal colonizers.Key words: biofilm, household washing machine, pyrosequencing, biodiversityCharakterisierung mikrobieller Gemeinschaften in Haushaltswaschmaschinen. Obwohl die mikrobielle Kolonisation von Haushaltsgeräten, insbesondere aufgrund des anhaltenden Trends hin zu niedrigen Wasch-und Reinigungstemperaturen, an Bedeutung gewinnen dürfte, ist wenig über die mikrobiellen Gemeinschaften in Haushaltswaschmaschinen bekannt. Im Rahmen dieser Studie wurden die mikrobiellen Gemeinschaften in der Einspülkammer und der Türdichtung von Waschmaschinen mittels 454-Pyrosequencing analysiert, wobei Proteobakterien als die dominanten prokaryotischen Besiedler ermittelt wurden, während Basidiomycota und Ascomycota die am häufigsten gefundenen Pilze innerhalb einer hoch diversen Gemeinschaft darstellten.

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“…Separate cells and small clusters are released more often, but large pieces are also released; the latter constitute a health hazard regarding the infectious dose (Stoodley et al 2001b). The released bacteria or their clusters drif within the liquid environment or are attached to damp semi-finished products and ready-made foodstuffs and can become sources of microbial contamination of other surfaces (Zottola & Sasahara 1994) or the products of both animal and vegetable origins (Rayner et al 2004). Besides the genetic regulation factors, physical forces present in the fluid environment of biofilm are involved.…”

Section: Biofilm Formationmentioning

confidence: 99%

Biofilms and hygiene on dairy farms and in the dairy industry: sanitation chemical products and their effectiveness on biofilms - a review

Vlkova¹,

Babák²,

Seydlova³

et al. 2008

Czech J. Food Sci.

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Vlková H., Babák V., Seydlová R., Pavlík I., Schlegelová J. (2008): Biofilms and hygiene on dairy farms and in the dairy industry: sanitation chemical products and their effectiveness on biofilms -a review. Czech J. Food Sci., 26: 309-323.Microbial biofilms which form on all types of surfaces of technological systems in the dairy industry and on dairy farms adversely affect the quality and safety of final products, i.e. both foodstuffs and raw materials used for their production. The fact that a number of microorganisms are alimentary pathogens, e.g. Staphylococcus aureus or Listeria monocytogenes, makes a serious problem directly affecting human health. Biofilms are usually formed by various species of microorganism, which protect each other against the effects of biocidal (antibacterial) agents and are resistant to these agents. The colonisation of surfaces of the open and closed piping systems, floors, waste, walls and ceilings of the production halls becomes a major problem in the selection of effective sanitation agents for their control. Based on the existing model studies, practical methods for testing the effectiveness of sanitation procedures should be evaluated, including the selection of biocides and comparison of the physical parameters of the sanitation procedures. Testing the effectiveness of the sanitation agents should be performed with the use of standardised tests, which consider microbial, structural, and chemical characteristics of the living microbial communities on specific contact surfaces in the food-processing industry.

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Prevalence of microbial biofilms on selected fresh produce and household surfaces

Cited by 80 publications

References 27 publications

Analysis of How a Biofilm Forms on the Surface of the Aquatic Macrophyte Phragmites australis

Analysis of How a Biofilm Forms on the Surface of the Aquatic Macrophyte Phragmites australis

Characterization of Microbial Communities in Household Washing Machines

Biofilms and hygiene on dairy farms and in the dairy industry: sanitation chemical products and their effectiveness on biofilms - a review

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