Prevalence of tetM, tetQ, nim and blaTEM genes in the oral cavities of Greek subjects: a pilot study

Ioannidis, Ioannis; Sakellari, Dimitra; Spala, Argyro; Arsenakis, Minas; Konstantinidis, Antonis

doi:10.1111/j.1600-051x.2009.01425.x

Cited by 26 publications

(26 citation statements)

References 53 publications

(50 reference statements)

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“…In our study, the most prevalent gene in periodontitis was tet ( Q ) while tet ( M ) showed a low prevalence. However, studies by Ioannidis et al [ 55 ] and Kim et al [ 56 ] reported both genes as the most frequent in diseased patients in Europe and the USA, respectively. In Dominican patients, tet ( 32 ) was the second most frequently detected gene in periodontitis; it is worth noting that, to our knowledge, this is the first study to report the presence of this gene in the subgingival biofilm of healthy and periodontally diseased patients.…”

Section: Discussionmentioning

confidence: 99%

Periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased Dominican adults

et al. 2015

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ObjectiveThe objective of this study was to compare the periodontopathogen prevalence and tetracycline resistance genes in Dominican patients with different periodontal conditions.MethodsSeventy-seven samples were collected from healthy, gingivitis, chronic (CP) and aggressive (AgP) periodontitis patients. Porphyromonas gingivalis, Treponema denticola, Tannerella forsythia, Aggregatibacter actinomycetemcomitans, Fusobacterium nucleatum, Prevotella intermedia, Parvimonas micra, Eikenella corrodens and Dialister pneumosintes and 11 resistance genes were studied by PCR. P. gingivalis fimA genotype was determined.ResultsIn healthy patients, P. micra and P. intermedia were the most and least frequently detected, respectively. T. forsythia and E. corrodens appeared in 100 % of gingivitis patients. Red complex, D. pneumosintes and E. corrodens were significantly more prevalent in CP compared to healthy patients. F. nucleatum and T. denticola were detected more frequently in AgP. A. actinomycetemcomitans was the most rarely observed in all groups. The fimA II genotype was the most prevalent in periodontitis patients. Seven tetracycline-resistant genes were detected. tet(Q), tet(32) and tet(W) showed the greatest prevalence. tet(32) was significantly more prevalent in CP than in healthy patients.ConclusionsRed complex bacteria and D. pneumosintes were significantly the most prevalent species among periodontitis patients. T. forsythia was the most frequently detected in this population. To our knowledge, this is the first study describing the tet(32) gene in subgingival biofilm from healthy and periodontally diseased subjects.Clinical relevanceThis study contributes to the knowledge on the subgingival microbiota and its resistance genes of a scarcely studied world region. Knowing the prevalence of resistance genes could impact on their clinical prescription and could raise awareness to the appropriate use of antibiotics.Electronic supplementary materialThe online version of this article (doi:10.1007/s00784-015-1516-2) contains supplementary material, which is available to authorized users.

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Section: Discussionmentioning

confidence: 99%

Periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased Dominican adults

et al. 2015

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“…In contrast, 7.7% of 26 subgingival P. gingivalis strains were reported to be β‐lactamase‐positive in one study , and 25.5% of 51 strains in another study were found in vitro to be resistant to amoxicillin, but susceptible to amoxicillin/clavulanic acid, indicative of β‐lactamase production. Additional β‐lactamase‐positive microbial species and subjects would probably be identified using more sensitive methods, such as molecular analysis of bacterial 16S ribosomal RNA gene sequences or β‐lactamase‐encoding genes , than the culture‐based procedures employed in the present study.…”

Section: Discussionmentioning

confidence: 99%

Prevalence of β‐lactamase‐producing bacteria in human periodontitis

Rams

Degener

Winkelhoff

2012

J of Periodontal Research

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The occurrence of β-lactamase-positive subgingival bacterial species in more than half of the subjects with severe chronic periodontitis raises questions about the therapeutic potential of single-drug regimens with β-lactam antibiotics in periodontal therapy. The in vitro effectiveness of metronidazole against nearly all recovered β-lactamase-producing subgingival bacterial species further supports clinical periodontitis treatment strategies involving the combination of systemic amoxicillin plus metronidazole.

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“…The experiment was set to have at least 90% power to detect changes of 25% with a significance level (alpha) of 0.05 (two-tailed). A pilot study provided the expected values of the primary outcome variable [ 16 ]. For clinical parameters, indicators of descriptive statistics were used, such as the mean and standard deviation for each group, with the patient as the observational unit.…”

Section: Materials and Methodologymentioning

confidence: 99%

Prevalence of β-lactam (blaTEM) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects

Koukos¹,

Konstantinidis²,

Tsalikis³

et al. 2016

TODENTJ

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Objectives:The aim of this study is to investigate the prevalence of blaTEM and nim genes that encode resistance to β-lactams and nitroimidazoles, respectively, in the oral cavity of systemically healthy Greek subjects.Materials and Methodology:After screening 720 potentially eligible subjects, 154 subjects were recruited for the study, including 50 periodontally healthy patients, 52 cases of gingivitis and 52 cases of chronic periodontitis. The clinical parameters were assessed with an automated probe. Various samples were collected from the tongue, first molars and pockets >6mm, and analysed by polymerase chain reaction-amplification of the blaTEM and nim genes, using primers and conditions previously described in the literature.Results:There was a high rate of detection of blaTEM in plaque and tongue samples alike in all periodontal conditions (37% of plaque and 60% of tongue samples, and 71% of participants). The blaTEM gene was detected more frequently in the tongue samples of the periodontally healthy (56%) and chronic periodontitis (62%) groups compared to the plaque samples from the same groups (36% and 29%, respectively; z-test with Bonferroni corrections-tests, P<0.05). The nim gene was not detected in any of the 343 samples analysed.Conclusion:The oral cavity of Greek subjects often harbours blaTEM but not nim genes, and therefore the antimicrobial activity of β-lactams might be compromised.

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Prevalence of tetM, tetQ, nim and bla_TEM genes in the oral cavities of Greek subjects: a pilot study

Abstract: Findings from the present study suggest a high prevalence of tetM, tetQ and bla(TEM), but not nim resistance genes in subgingival and tongue samples from Greek subjects.

Cited by 26 publications

References 53 publications

Periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased Dominican adults

Periodontal pathogens and tetracycline resistance genes in subgingival biofilm of periodontally healthy and diseased Dominican adults

Prevalence of β‐lactamase‐producing bacteria in human periodontitis

Prevalence of β-lactam (blaTEM) and Metronidazole (nim) Resistance Genes in the Oral Cavity of Greek Subjects

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