Prevalence of<i>gyrA</i>Mutations in Nalidixic Acid-Resistant Strains of<i>Salmonella</i>Enteritidis Isolated from Humans, Food, Chickens, and the Farm Environment in Brazil

Campioni, Fábio; Souza, Roberto A.; Martins, Vinicius Vicente; Stehling, Eliana Guedes; Bergamini, Alzira Maria Morato; Falcão, Juliana Pfrimer

doi:10.1089/mdr.2016.0024

Cited by 30 publications

(22 citation statements)

References 43 publications

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“…This fact was not detectable by the disc‐diffusion technique in previous studies of our research group with the serovar Enteritidis, which demonstrates that S . Dublin strains might present resistance mechanisms different from the ones found in that study (Campioni et al., ).…”

Section: Discussioncontrasting

confidence: 66%

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Vilela¹,

Frazão

Rodrigues

et al. 2017

Zoonoses and Public Health

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SummarySalmonella Dublin is strongly adapted to cattle causing enteritis and/or systemic disease with high rates of mortality. However, it can be sporadically isolated from humans, usually causing serious disease, especially in patients with underlying chronic diseases.The aim of this study was to molecularly type S. Dublin strains isolated from humans and animals in Brazil to verify the diversity of these strains as well as to ascertain possible differences between strains isolated from humans and animals. Moreover, the presence of the capsular antigen Vi and the plasmid profile was characterized in addition to the anti-microbial resistance against 15 drugs. For this reason, 113 S. Dublin strains isolated between 1983 and 2016 from humans (83) and animals (30) in Brazil were typed by PFGE and MLVA. The presence of the capsular antigen Vi was verified by PCR, and the phenotypic expression of the capsular antigen was determined serologically. Also, a plasmid analysis for each strain was carried out. The strains studied were divided into 35 different PFGE types and 89 MLVA-types with a similarity of ≥80% and ≥17.5%, respectively. The plasmid sizes found ranged from 2 to >150 kb and none of the strains studied presented the capsular antigen Vi. Resistance or intermediate resistance was found in 23 strains (20.3%) that were resistant to ampicillin, ciprofloxacin, chloramphenicol, imipenem, nalidixic acid, piperacillin, streptomycin and/or tetracycline. The majority of the S. Dublin strains studied and isolated over a 33-year period may descend from a common subtype that has been contaminating humans and animals in Brazil and able to cause invasive disease even in the absence of the capsular antigen. The higher diversity of resistance phenotypes in human isolates, as compared with animal strains, may be a reflection of the different anti-microbial treatments used to control S. Dublin infections in humans in Brazil. K E Y W O R D Santigen Vi, anti-microbial resistance, multiple-locus variable-number tandem-repeat analysis, plasmid analysis, pulsed-field gel electrophoresis, Salmonella Dublin

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Section: Discussioncontrasting

confidence: 66%

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Vilela¹,

Frazão

Rodrigues

et al. 2017

Zoonoses and Public Health

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“…Overall, qnrB and qnrS are the most prevalent qnr families detected. Different qnrB variants (when specified recognized as qnrB2, qnrB5, qnrB19) have been reported in E. coli, E. fergusonii, K oxytoca, S. Corvallis, S. Schwarzengund, and S. Newport isolates obtained from chicken or chicken meat [36,140,147,[175][176][177][178]. qnrS was reported in E. coli obtained from chicken meat and swabs from healthy poultry and pork [140,177,179].…”

Section: Colistin Resistance-mcrmentioning

confidence: 99%

Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

Rabello

Bonelli

Penna

et al. 2020

Animals

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In animal husbandry, antimicrobial agents have been administered as supplements to increase production over the last 60 years. Large-scale animal production has increased the importance of antibiotic management because it may favor the evolution of antimicrobial resistance and select resistant strains. Brazil is a significant producer and exporter of animal-derived food. Although Brazil is still preparing a national surveillance plan, several changes in legislation and timely programs have been implemented. Thus, Brazilian data on antimicrobial resistance in bacteria associated with animals come from official programs and the scientific community. This review aims to update and discuss the available Brazilian data on this topic, emphasizing legal aspects, incidence, and genetics of the resistance reported by studies published since 2009, focusing on farm animals and derived foods with the most global public health impact. Studies are related to poultry, cattle, and pigs, and mainly concentrate on non-typhoid Salmonella, Escherichia coli, and Staphylococcus aureus. We also describe legal aspects of antimicrobial use in this context; and the current occurrence of genetic elements associated with resistance to beta-lactams, colistin, and fluoroquinolones, among other antimicrobial agents. Data here presented may be useful to provide a better understanding of the Brazilian status on antimicrobial resistance related to farm animals and animal-derived food products.

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“…Acquisition of plasmid-mediated resistant genes is the principal mechanism for antimicrobial resistance in Salmonella. However, spontaneous gene mutation does contribute to the emergence of resistant Salmonella strains especially to quinolones [12,13].…”

Section: Discussionmentioning

confidence: 99%

Tylosin exposure reduces the susceptibility of Salmonella Typhimurium to florfenicol and tetracycline

Mechesso

Park

2020

BMC Vet Res

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Background: Antibiotics exposure has been implicated in the emergence of bacterial strains that are resistant to structurally related or unrelated antibiotics. Tylosin is a macrolide antibiotic that has been administered to treat respiratory pathogenic bacteria in swine. Thus, this study was undertaken to evaluate the impact of exposure to a constant (3 μg/mL) and decreasing concentrations of tylosin on the susceptibility of Salmonella enterica serovar Typhimurium to various antibiotics. Results: S. Typhimurium strains exposed to tylosin for 12 and 24 h in the in vitro dynamic model demonstrated at least an eight-fold increase in the minimum inhibitory concentrations (MICs) of florfenicol and tetracycline. Exposure to tylosin extended the lag-time of the growth curve and enhanced the generation of reactive oxygen species. Gene expression analysis demonstrated up-regulation of the acrAB and tolC Salmonella efflux pump genes and its global regulators (marA and soxS). Besides, the expression of ompC gene was down-regulated in tylosin exposed S. Typhimurium. Conclusion: Exposure to decreasing concentrations of tylosin could reduce the susceptibility of S. Typhimurium to florfenicol and tetracycline.

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Prevalence ofgyrAMutations in Nalidixic Acid-Resistant Strains ofSalmonellaEnteritidis Isolated from Humans, Food, Chickens, and the Farm Environment in Brazil

Cited by 30 publications

References 43 publications

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Genetic diversity, anti‐microbial resistance, plasmid profile and frequency of the Vi antigen in Salmonella Dublin strains isolated in Brazil

Antimicrobial Resistance in Farm Animals in Brazil: An Update Overview

Tylosin exposure reduces the susceptibility of Salmonella Typhimurium to florfenicol and tetracycline

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