Prevalence ofEimeriaspp. in Broilers by Multiplex PCR in the Southern Region of Brazil on Two Hundred and Fifty Farms

Abstract

Parasitic infections caused by Eimeria species are responsible for most economic losses in poultry production. Prevalence studies can adequately assist the design of prophylaxis strategies for disease control. Therefore, stool samples from 251 flocks of broilers from 28 to 48 days old were collected in 21 municipalities in the state of Santa Catarina, Brazil, to detect and examine the prevalence of Eimeria acervulina, Eimeria maxima, Eimeria tenella, Eimeria mitis, Eimeria praecox, Eimeria necatrix, and Eimeria brunetti. The oocysts were recovered and quantified, and the species were identified by a multiplex PCR technique. Amplicons of seven Eimeria species originating from the PCR-positive samples were cloned. Microscopy studies demonstrated that 96% of the farms were positive for the Eimeria. Seven species were identified, as follows: E. maxima (63.7%) and E. acervulina (63.3%) were the most prevalent species, followed by E. tenella (54.6%), E. mitis (38.6%), E. praecox (25.1%), E. necatrix (24.3%), and E. brunetti (13.1%). The average number of species detected per farm was 2.96, and the most common were E. acervulina, E. maxima, and E. tenella (9.16%). The sequencing of the clones confirmed the specificity and effectiveness of multiplex PCR for the identification of seven species of Eimeria, so this tool can be useful in studying circulating species in poultry farms, thereby assisting prophylactic measures against coccidiosis.

Prevalence of Eimeria species in domestic chickens in Anhui province, China

“…Additionally, different types animal handling practices can also affect the prevalence of Eimeria species. The overall prevalence of coccidian infection obtained in this study (87.72%), was lower than the 96% found in the Southern Region of Brazil (Moraes et al 2015). However, it was higher than earlier reports from some areas: 81.03% in two north Indian states viz., Uttar Pradesh and Uttarakhand (Kumar et al 2015), 31.5% in Khuzestan, southwest Iran (Hamidinejat et al 2010), and 66.8% in Karbala and Babylon province, Iraq (Hamza et al 2015).…”

Chicken Coccidiosis in Central Java, Indonesia: A Recent Update

“…are evidently spread worldwide [ 17 ]. Eimeria species have been found with different prevalence during regional surveys in France [ 18 ], Jordan [ 19 ], Romania [ 20 ], Brazil [ 21 ], or China [ 22 ]. Our findings are in agreement with previous reports that show E. tenella as the predominant species found in Asia [ 23 , 24 ].…”

Coccidiosis en pollos de engorda comerciales en Brazil entre 2012 y 2019: especies principales y grados de daño

“…Los estudios que informan las características epidemiológicas de la coccidiosis en Brasil son raros. Es importante prestar atención a las especies que se encuentran en el territorio; nuestros hallazgos concuerdan con los de otros autores que reportaron frecuentemente E. acervulina, E. maxima y E. tenella (Moraes et al, 2015;Chapman et al, 2016;Kim et al, 2017); estas especies, además de ser reportadas con mayor frecuencia, son las que comúnmente desarrollan resistencia a las drogas sintéticas (Shivaramaiah et al, 2014).…”