Prevalence of<i>Demodex canis-</i>positive healthy dogs at trichoscopic examination

Fondati, Alessandra; Lucia, Michela De; Furiani, Nicla; Monaco, Moira; Ordeix, Laura; Scarampella, Fabia

doi:10.1111/j.1365-3164.2009.00769.x

Cited by 25 publications

(27 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Rodríguez-Vivas et al (2003) could only find Demodex mites in skin scrapings in 3% of healthy dogs in Korea. Fondati et al (2009), using trichoscopy, could not detect D. canis mites in any of the 70 dogs examined, and in only one dog did they I. Ravera and L. Altet contributed equally to this work. I. Ravera : M. Bardagí : L. Ferrer (*) Departament de Medicina i Cirurgia Animals, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona, Spain e-mail: lluis.ferrer@uab.cat find a D. injai mite.…”

Section: Introductionmentioning

confidence: 87%

Development of a real-time PCR to detect Demodex canis DNA in different tissue samples

et al. 2010

View full text Add to dashboard Cite

The present study reports the development of a real-time polymerase chain reaction (PCR) to detect Demodex canis DNA on different tissue samples. The technique amplifies a 166 bp of D. canis chitin synthase gene (AB 080667) and it has been successfully tested on hairs extracted with their roots and on formalin-fixed paraffin embedded skin biopsies. The real-time PCR amplified on the hairs of all 14 dogs with a firm diagnosis of demodicosis and consistently failed to amplify on negative controls. Eleven of 12 skin biopsies with a morphologic diagnosis of canine demodicosis were also positive. Sampling hairs on two skin points (lateral face and interdigital skin), D. canis DNA was detected on nine of 51 healthy dogs (17.6%) a much higher percentage than previously reported with microscopic studies. Furthermore, it is foreseen that if the number of samples were increased, the percentage of positive dogs would probably also grow. Moreover, in four of the six dogs with demodicosis, the samples taken from non-lesioned skin were positive. This finding, if confirmed in further studies, suggests that demodicosis is a generalized phenomenon in canine skin, due to proliferation of local mite populations, even though macroscopic lesions only appear in certain areas. The real-time PCR technique to detect D. canis DNA described in this work is a useful tool to advance our understanding of canine demodicosis.

show abstract

Section: Introductionmentioning

confidence: 87%

Development of a real-time PCR to detect Demodex canis DNA in different tissue samples

et al. 2010

View full text Add to dashboard Cite

show abstract

“…4 Immune function is suspected to play a role in the manifestation of clinical demodicosis in numerous species. For instance, the occurrence of this disease in young dogs has been attributed to incomplete immune system development, 8 while Demodex folliculitis infestations occur in older, immunosuppressed humans. 7 Unlike some species, such as domestic dogs and African buffalo (Syncerus caffer), in which clinical demodicosis is thought to be more common in young animals, 12,29 demodectic mange was more commonly observed in adult white-tailed deer in the present study.…”

Section: Discussionmentioning

confidence: 99%

Demodectic Mange, Dermatophilosis, and Other Parasitic and Bacterial Dermatologic Diseases in Free-Ranging White-tailed Deer (Odocoileus virginianus) in the United States From 1975 to 2012

et al. 2013

View full text Add to dashboard Cite

The white-tailed deer (Odocoileus virginianus) is a common and widespread North American game species. To evaluate the incidence, clinical manifestations, demography, and pathology of bacterial and parasitic dermatologic diseases in white-tailed deer in the southeastern United States, we retrospectively evaluated white-tailed deer cases submitted to the Southeastern Cooperative Wildlife Disease Study from 1975 to 2012. Among 2569 deer examined, bacterial or parasitic dermatologic disease was diagnosed in 88 (3.4%) individuals, with Demodex spp (n ¼ 37; 42.0%) and Dermatophilus congolensis (n ¼ 19; 21.6%) as the most common causes. Demodicosis was significantly more common in deer older than 2 years and was most often detected in the fall; no statistically significant sex predilection was identified. Affected animals had patchy to generalized alopecia, often distributed over the head, neck, limbs, and trunk; microscopic lesions included epidermal crusts and cutaneous nodules with mild perifollicular, lymphoplasmacytic inflammation. Dermatophilosis was most common in males younger than 1 year that were often found dead. Crusting, erythema, and alopecia occurred on the face, ears, and distal extremities. Less commonly, infectious dermatologic diseases were associated with other bacteria (n ¼ 13; 14.8%), fungi (n ¼ 5; 5.7%), ectoparasites (chiggers, lice, mites, and ticks; n ¼ 11; 12.5%), and larval nematodes (n ¼ 7; 8.0%). Population-level effects of these diseases in white-tailed deer are likely minimal; however, due to their dramatic presentation, demodicosis, dermatophilosis, and other infectious skin diseases can be of concern to hunters and, in some cases, may have zoonotic potential.

show abstract

“…3,4 However, trichograms are less sensitive than skin scraping, 4 even though tricoscopic examination has been recommended as a reliable diagnostic method 2 and recently indicated for evaluation of the prevalence of demodectic mites in healthy dogs. 5 Acetate tape impression is also recommended for the detection of extrafollicular mites 6 and can be applied after deep skin scraping to the same lesion. 3,7 Therefore, the aim of the present study was to evaluate and compare the sensitivities of acetate tape impression with skin squeezing and deep skin scraping for the diagnosis of canine demodicosis.…”

mentioning

confidence: 99%

Comparison of acetate tape impression with squeezing versus skin scraping for the diagnosis of canine demodicosis

Pereira

Gremião

et al. 2012

Aust Veterinary J

View full text Add to dashboard Cite

This study compared the sensitivity of acetate tape impression and skin squeezing with that of deep skin scraping for the diagnosis of demodicosis in dogs. Demodex canis was detected in 100% of acetate tape impressions obtained after skin squeezing and in 90% of deep skin scrapings. There was a significant difference (P < 0.001) between the techniques in the total number of mites detected. Acetate tape impression with skin squeezing was found to be more sensitive than deep skin scraping and is an alternative diagnostic method for canine demodicosis.

show abstract

Prevalence ofDemodex canis-positive healthy dogs at trichoscopic examination

Cited by 25 publications

References 17 publications

Development of a real-time PCR to detect Demodex canis DNA in different tissue samples

Development of a real-time PCR to detect Demodex canis DNA in different tissue samples

Demodectic Mange, Dermatophilosis, and Other Parasitic and Bacterial Dermatologic Diseases in Free-Ranging White-tailed Deer (Odocoileus virginianus) in the United States From 1975 to 2012

Comparison of acetate tape impression with squeezing versus skin scraping for the diagnosis of canine demodicosis

Contact Info

Product

Resources

About