Prevalence of human papillomavirus and Epstein-Barr virus DNA in penile cancer cases from Brazil

Afonso, Larissa Alves; Moysés, Natalia; Alves, Gilda; Ornellas, Antônio Augusto; Passos, Mauro Romero Leal; Oliveira, Ledy H. S.; Cavalcanti, Sílvia M. B.

doi:10.1590/s0074-02762012000100003

Cited by 30 publications

(38 citation statements)

References 25 publications

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“…This fact might also be the cause of our high number of at least one high-risk HPV type infection (58%) among our population. Regarding the observed high prevalence of high-risk HPV types among our studied population, it was also observed by others and may be consequence of the local restrict HPV vaccination policies (14).…”

Section: Discussionsupporting

confidence: 85%

Genital prevalence of HPV types and co-infection in men

et al. 2014

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ARTICLE INFO ______________________________________________________________ ______________________Introduction: HPV infection is a highly prevalent sexually transmitted disease and there is evidence of the relationship of HPV infection and the development of genital warts, penile intraepitelial neoplasia, invasive penile carcinoma and cervical cancer. However, there is sparse data regarding the prevalence of HPV types and co-infection of different HPV types among men. Objectives: To assess the prevalence of HPV subtypes infections and rates of co-infection among men. Materials and Methods: 366 men were evaluated from March to October 2010. Men were referred to our institution for HPV diagnostic evaluation based on the following criteria: 1. presence of a genital wart; 2. presence of an atypical genital lesion; 3. absence of symptoms and a partner with a HPV diagnosis; 4. absence of symptoms and a desire to undergo a full STD diagnostic evaluation. Genital samples were collected from the urethra, penile shaft, scrotum and anus with Digene® collection and preservation kit and submitted to HPV genotype microarray detection (Papillocheck®). All men were tested for the low-risk HPV types 6-11-40-42-43-44 and for the high-risk HPV types 16-18-31-33-35-39-45-51-52-53-56-58-59-66-68-70-73-82. Results: Of the 366 men, 11 were tested inconclusive and were excluded from the analysis. 256 men (72.1% of the men from the cohort referred to our institution) tested positive with genotype micro-array detection and 99 tested negative. The most prevalent HPV-subtypes in the studied population were 6, 42, 51 and 16. Co-infection was found in 153 men. Of those, 70 (19.7%) had a co-infection by 2 types, 37 (10.4%) by 3 types; 33 men (9.2%) by 4 types; 8 men (2.2%) by 5 types; 1 man (0.3%) by 6 types; 1 man (0.3%) by 7 types; 2 men (0.6%) by 8 types and 1 man (0.3%) by 9 types. Conclusion: The most frequent HPV types were 6, 16, 42 and 51. Co-infection was found in 59% of our patients. This information is vital to drive future public health policies including massive public vaccination campaign.

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Section: Discussionsupporting

confidence: 85%

Genital prevalence of HPV types and co-infection in men

et al. 2014

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“…In our patients, the downregulation of C3 and C4 could be caused by HPV and/or EBV infection, viruses that are highly prevalent in SCCP lesions (26). Viral proteins counteract the immune response (27).…”

Section: Relative Intensitymentioning

confidence: 77%

Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis

Ornellas¹,

Ornellas²,

Chinello³

et al. 2012

Int. braz j urol.

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Purpose: To investigate the use of ClinProt technique to identify cancer markers in plasma of patients suffering from squamous cell carcinoma of the penis (SCCP). Materials and Methods: Plasma of 36 healthy subjects and 25 patients with penile carcinoma who underwent surgical treatment between June 2010 and June 2011 was collected and analyzed by the ClinProt/MALDI/ToF technique. Then the peptides were identifi ed from the C8 MB eluted fraction of patients' and control subjects' plasma by LIFT MS/MS. Results: A cluster of 2 peptides (A=m/z 1897.22 ± 9 Da and B=m/z 2021.99 ± 9 Da) was able to discriminate patients from control subjects. Cross validation analysis using the whole casuistic showed 62.5% and 86.76% sensitivity and specifi city, respectively. The cluster also showed very high sensitivity (100%) and specifi city (97%) for SCCP patients that died due to the disease. Furthermore, patients with lymph node involvement presented sensitivity and specifi city of 80% and 97%, respectively. These two peptides were identifi ed by the proteomic approach based on a MALDI-TOF/TOF as fragments of C3 (m/z 1896.17) and C4a/b (m/z 2021.26) complement proteins. Conclusions:The results showed that as the disease progresses, the fragments C3 and C4 A/B are less expressed in comparison with healthy subjects. These results may be useful as prognostic tools.

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“…DNA was precipitated with 300µL 0.3 M sodium acetate plus 900µL of ice-cold ethanol, washed with 70% ethanol, air dried, and suspended in 50 µl sterile water (Afonso et al, 2012).…”

Section: Dna Extraction and Analysismentioning

confidence: 99%

“…Each cycle comprised denaturation at 94°C for 1 min, annealing at 55°C for 2 min, and chain elongation at 72°C for 2 min. The beta-actin primers Ac1 and Ac2 (0.1 pmol each), which amplify a 330-bp region of human DNA, were used as internal sample controls (Afonso et al 2012).. Specific genotyping was performed by PCR amplification with primers from the E6 gene DNA sequences of Low risk HPVs 6, 11and 53, and High risk HPVs 16,18,31,33,35,45,56,and 58 7 Thirty-five amplification cycles were carried out in 50 µl reaction mixture with denaturation at 94°C for 30 s, annealing at 55°C for 30 s, and chain elongation at 72°C for 1 min (Afonso et al 2012)..…”

Section: Dna Extraction and Analysismentioning

confidence: 99%

“…Few studies have stated HPV involvement in penile carcinoma. Specific HPV genotypes, predominantly HR-HPVs 16 and 18, have been identified in 40-70% of malignant penile lesions (Afonso et al 2012). Nevertheless, HR-HPV has been identified as the etiological agent of 90-96% of anal carcinomas, and persistent infection is a major independent risk factor.…”

Section: Introductionmentioning

confidence: 99%

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High-Risk Human Papillomavirus Infection and P16 Expression in Men With Anogenital Lesions Attending a Dermatology Clinic

Dobao¹,

Afonso²,

Rocha³

et al. 2014

VR&R

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Our study comprises a cases series of 71 men attending a dermatology clinic in Brazil during an 18-month period with anogenital HPV infection. Clinical manifestations, laboratory findings and sociodemographic factors were evaluated. Biopsy samples were subjected to histopathological analysis, generic and type-specific viral identification, and p16 INK4a quantification. The average age at diagnosis was 33 years. We observed little variation in identified viral types (HPVs 6, 11, 16 and 53), despite the inclusion of 16 HIV+ patients. The presence of high-risk HPV was associated with receptive anal sex (p<0.05), lesion malignancy (p<0.01) and p16INK4a expression (p<0.05). HIV+ was correlated with HPV16 infection, presence of perianal lesions and high-grade lesions (p<0.05) diagnosed at a younger mean age than HIV-patients (p<0.05). Our results demonstrate the unequivocal relationship between high-risk HPV infection and the presence of high-grade lesions, HPV 16 tropism in the anal epithelium, and the role of receptive anal sex as a risk factor for development of high-grade anal lesions, that present early in HIV+ men who have sex with men. As the 5 high-grade lesions (1 AINII and 4 AIN III) showed p16INK4a negativity but were related to HPV 16 presence, we believe that p16INK4a is a promising biomarker, but its use remains controversial requiring further research.

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Prevalence of human papillomavirus and Epstein-Barr virus DNA in penile cancer cases from Brazil

Cited by 30 publications

References 25 publications

Genital prevalence of HPV types and co-infection in men

Genital prevalence of HPV types and co-infection in men

Downregulation of C3 and C4A/B complement factor fragments in plasma from patients with squamous cell carcinoma of the penis

High-Risk Human Papillomavirus Infection and P16 Expression in Men With Anogenital Lesions Attending a Dermatology Clinic

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