Prevalence of honey bee ( Apis mellifera ) viruses in temperate and subtropical regions from Argentina

Molineri, Ana; Pacini, Adriana; Giacobino, Agostina; Cagnolo, Natalia Bulacio; Aignasse, Andrea; Zago, Luis; Fondevila, Norberto; Ferrufino, Cecilia; Merke, Julieta; Orellano, Emanuel; Bertozzi, Ezequiel; Pietronave, Hernán; Signorini, Marcelo

doi:10.1016/j.ram.2016.12.004

Cited by 15 publications

(19 citation statements)

References 27 publications

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“…Asymptomatic or covert infections seem to be very common in honey bees, for example, about 15% of the bees assessed in this study were infected with acute and/or chronic paralysis virus, some at relatively high concentrations; however, none of them showed visible signs of disease (impaired movements, trembling, above‐average loss of hair). This absence of disease symptoms in infected bees was noted already when the paralysis viruses were first described (Bailey, Gibbs, & Woods, ) and affirmed in many subsequent publications, for example, Gauthier et al (); Hung, Shimanuki, and Knox (); Molineri et al (); Nielsen et al (); and Tentcheva et al (). Gauthier et al () quantified viral loads in asymptomatic bees by qPCR and found high concentrations similar to and even exceeding those reported here.…”

Section: Discussionmentioning

confidence: 58%

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Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

D’Alvise

Seeburger

Gihring

et al. 2019

Ecology and Evolution

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The health of the honey bee Apis mellifera is challenged by introduced parasites that interact with its inherent pathogens and cause elevated rates of colony losses. To elucidate co‐occurrence, population dynamics, and synergistic interactions of honey bee pathogens, we established an array of diagnostic assays for a high‐throughput qPCR platform. Assuming that interaction of pathogens requires co‐occurrence within the same individual, single worker bees were analyzed instead of collective samples. Eleven viruses, four parasites, and three pathogenic bacteria were quantified in more than one thousand single bees sampled from sixteen disease‐free apiaries in Southwest Germany. The most abundant viruses were black queen cell virus (84%), Lake Sinai virus 1 (42%), and deformed wing virus B (35%). Forager bees from asymptomatic colonies were infected with two different viruses in average, and simultaneous infection with four to six viruses was common (14%). Also, the intestinal parasites Nosema ceranae (96%) and Crithidia mellificae/Lotmaria passim (52%) occurred very frequently. These results indicate that low‐level infections in honey bees are more common than previously assumed. All viruses showed seasonal variation, while N. ceranae did not. The foulbrood bacteria Paenibacillus larvae and Melissococcus plutonius were regionally distributed. Spearman's correlations and multiple regression analysis indicated possible synergistic interactions between the common pathogens, particularly for black queen cell virus. Beyond its suitability for further studies on honeybees, this targeted approach may be, due to its precision, capacity, and flexibility, a viable alternative to more expensive, sequencing‐based approaches in nonmodel systems.

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Section: Discussionmentioning

confidence: 58%

“…et al (2007);Hung, Shimanuki, and Knox (1996);Molineri et al (2017);Nielsen et al (2008); andTentcheva et al (2004) Gauthier et al (2007). …”

mentioning

confidence: 99%

Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

D’Alvise

Seeburger

Gihring

et al. 2019

Ecology and Evolution

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“…The previously developed TaqMan assays for ABPV, BQCV, CBPV, and DWV showed good performance and reliability also for the detection and quantification of a broad genetic range of previously determined Slovenian honeybee viral strains [ 4 , 14 , 28 ]. The newly developed TaqMan RT-qPCR assay for detecting and quantifying SBV from this study is a good alternative and improvement of previously described assays, in terms of SYBR Green or MGB-probes [ 30 , 42 , 43 , 44 ], while SYBR Green also provides non-specific detection, and MGB-probes are not as stable as TaqMan probes. Because of this, TaqMan probes are the most frequently used in routine diagnostic laboratories for diagnostics of infectious diseases [ 45 , 46 , 47 ].…”

Section: Discussionmentioning

confidence: 99%

The Comparison of Honeybee Viral Loads for Six Honeybee Viruses (ABPV, BQCV, CBPV, DWV, LSV3 and SBV) in Healthy and Clinically Affected Honeybees with TaqMan Quantitative Real-Time RT-PCR Assays

Šimenc

Knific

Toplak

2021

Viruses

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The viral loads of acute bee paralysis virus (ABPV), black queen cell virus (BQCV), chronic bee paralysis virus (CBPV), deformed wing virus (DWV), Lake Sinai virus 3 (LSV3), and sacbrood bee virus (SBV) were determined in samples with the use of quantitative TaqMan real-time reverse transcription and polymerase chain reaction (RT-qPCR). A total of 108 samples of healthy adult honeybees from four differently located apiaries and samples of honeybees showing different clinical signs of viral infections from 89 apiaries were collected throughout Slovenia. The aim of this study was to discover correlations between viral loads and clinical signs in adult honeybees and confirm previously set threshold viral load levels between healthy and clinically affected honeybees. Within this study, two new RT-qPCR assays for quantification of LSV3 and SBV were developed. Statistically significant differences in viral loads of positive samples were identified between healthy and clinically affected honeybees for ABPV, CBPV, DWV, and SBV, while for BQCV and LSV3, no statistical differences were observed between both groups. Despite high detected LSV3 prevalence and viral loads around 6.00 log10 viral copies/bee, this lineage probably has a limited impact on the health status of honeybee colonies. The determined viral loads between 3.94 log10 and 13.17 log10 in positive samples for six viruses, collected over 10 consecutive months, including winter, present additional information of high viral load variations in healthy honeybee colonies.

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“…6(1).2020 ABPV was detected with high prevalence percentage (21.5%) in Argentinean apiaries. In addition to its detection, co-infections with ABPV and BQCV were reported [9]. However, despite increasing knowledge on bee viruses, the information about the presence and effect of virus in Argentinean queen rearing apiaries is lacking.…”

Section: Mod Concep Dev Agronomentioning

confidence: 99%

“…In addition, previous reports demonstrated that the virus present in different bee food sources (honey, pollen and royal jelly) may be transmitted horizontally between adult bees such as queens, workers, or drones [6]. Recent studies showed the presence of virus in honeybee colonies from Argentinean producers located along temperate and subtropical regions [7][8][9]. Notably, many honeybee viruses have been detected in Argentinian stingless bees and bumblebees [10,11].…”

Section: Introductionmentioning

confidence: 99%

Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina

Ferrufino¹,

Gonzalez²,

Santos³

et al. 2020

MCDA

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Centro de investigaciones en CienciasAgronómicas y Veterinarias (CICVyA), Argentina Recently, BQCV was detected in Argentinean apiaries located along different geographic regions, with a prevalence of 8% [8]. Acute bee paralysis virus (ABPV) is a widespread bee pathogen that has been linked with colony losses [14]. Previous reports demonstrated highly increased percentage of infection by ABPV in Slovenian queen rearing apiaries [5]. ABPV usually shows low prevalence values and its detection is not associated with clinical signs. However, when the virus is present at high titers, elevated mortality rates are evident [14].

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Prevalence of honey bee ( Apis mellifera ) viruses in temperate and subtropical regions from Argentina

Cited by 15 publications

References 27 publications

Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

Seasonal dynamics and co‐occurrence patterns of honey bee pathogens revealed by high‐throughput RT‐qPCR analysis

The Comparison of Honeybee Viral Loads for Six Honeybee Viruses (ABPV, BQCV, CBPV, DWV, LSV3 and SBV) in Healthy and Clinically Affected Honeybees with TaqMan Quantitative Real-Time RT-PCR Assays

Detection of Honeybee Viruses in a Queen-Rearing Apiary from Argentina

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