Prevalence of fluoroquinolone, macrolide and sulfonamide-related resistance genes in landfills from East China, mainly driven by MGEs

Yao, Lihua; Li, Yue; Li, Zhangqiang; Shen, Dongsheng; Feng, Huajun; Zhou, Hong; Wang, Meizhen

doi:10.1016/j.ecoenv.2019.110131

Cited by 43 publications

(10 citation statements)

References 53 publications

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“…Previous study revealed that the relative abundance of ARGs (sulI, sulII, blaTEM-1,tetA, tetO, tetQ, and tetW) ranged from 10 -6 to 10 -1 gene copies per 16S rRNA in the four major Chinese carp, while the relative abundance of ARGs (qac, sul, erm and tetM) ranged from 1.46 × 10 -7 to 4.6 × 10 1 www.nature.com/scientificreports/ copies/ 16S rRNA in raw and fresh foods 16,40 . Yao et al also reported that the relative abundances of qnrD, qnrS, mexF, ermA, ermB, mefA, sul1, and sul2 in most landfills were > 10 -4 copies per 16S rRNA, suggesting the presence of highly contaminated ARGs 39 . Therefore, we should pay more attention on the ARGs with relative abundance > 10 -4 copies per 16S rRNA.…”

Section: Discussionmentioning

confidence: 97%

“…The relative abundances of the 10 ARG subtypes ranged from 7.55 × 10 –5 to 3.42 × 10 –3 copy of ARG/ copy of 16S rRNA gene. Typically, the relative abundance of ARGs in unpolluted environments ranges from 10 –8 to 10 –6 copies/16S rRNA, whereas concentrations in highly contaminated sites are often several orders of magnitude higher (e.g., 10 –4 ) 39 . Previous study revealed that the relative abundance of ARGs ( sulI , sulII , blaTEM-1 , tetA , tetO , tetQ , and tetW ) ranged from 10 –6 to 10 –1 gene copies per 16S rRNA in the four major Chinese carp, while the relative abundance of ARGs ( qac , sul , erm and tetM ) ranged from 1.46 × 10 –7 to 4.6 × 10 1 copies/ 16S rRNA in raw and fresh foods 16 , 40 .…”

Section: Discussionmentioning

confidence: 99%

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Metagenomic characterization of bacterial community and antibiotic resistance genes in representative ready-to-eat food in southern China

Cao

Liang

et al. 2020

Sci Rep

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Ready-to-eat (RTE) foods have been considered to be reservoirs of antibiotic resistance bacteria, which constitute direct threat to human health, but the potential microbiological risks of RTE foods remain largely unexplored. In this study, the metagenomic approach was employed to characterize the comprehensive profiles of bacterial community and antibiotic resistance gene (ARG) in 18 RTE food samples (8 RTE meat, 7 RTE vegetables and 3 RTE fruit) in southern China. In total, the most abundant phyla in RTE foods were Proteobacteria, Firmicutes, Cyanobacteria, Bacteroidetes and Actinobacteria. 204 ARG subtypes belonging to 18 ARG types were detected with an abundance range between 2.81 × 10−5 and 7.7 × 10−1 copy of ARG per copy of 16S rRNA gene. Multidrug-resistant genes were the most predominant ARG type in the RTE foods. Chloramphenicol, macrolide-lincosamide-streptogramin, multidrug resistance, aminoglycoside, bacitracin, tetracycline and β-lactam resistance genes were dominant, which were also associated with antibiotics used extensively in human medicine or veterinary medicine/promoters. Variation partitioning analysis indicated that the join effect of bacterial community and mobile genetic elements (MGEs) played an important role in the resistome alteration. This study further deepens the comprehensive understanding of antibiotic resistome and the correlations among the antibiotic resistome, microbiota, and MGEs in the RTE foods.

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Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

Metagenomic characterization of bacterial community and antibiotic resistance genes in representative ready-to-eat food in southern China

Cao

Liang

et al. 2020

Sci Rep

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“…With the overuse, misuse, and abuse of antibiotics in modern medicine and the livestock industry in the last decades ( Yao L. et al, 2020 ; Zhang et al, 2020 ; Zou et al, 2020 ; Wang et al, 2021 ), antimicrobial resistance has developed into one of the most urgent public health crises in recent years ( Zhu et al, 2017a ; Vos et al, 2019 ; Joshi and Kim, 2020 ; Dai et al, 2022 ). According to statistics, the global defined daily doses of antibiotics reached 34.8 billion in 2015 and have increased by 65% since 2000 ( Zhao R. et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

Environmental drivers and interaction mechanisms of heavy metal and antibiotic resistome exposed to amoxicillin during aerobic composting

Liu

Li²,

Su³

et al. 2023

Front. Microbiol.

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The environmental accumulation and spread of antibiotic resistance pose a major threat to global health. Aerobic composting has become an important hotspot of combined pollution [e.g., antibiotic resistance genes (ARGs) and heavy metals (HMs)] in the process of centralized treatment and resource utilization of manure. However, the interaction mechanisms and environmental drivers of HMs resistome (MRGs), antibiotic resistance (genotype and phenotype), and microbiome during aerobic composting under the widely used amoxicillin (AMX) selection pressure are still poorly understood. Here, we investigated the dynamics of HMs bioavailability and their MRGs, AMX-resistant bacteria (ARB) and antibiotic resistome (ARGs and intI1), and bacterial community to decipher the impact mechanism of AMX by conducting aerobic composting experiments. We detected higher exchangeable HMs and MRGs in the AMX group than the control group, especially for the czrC gene, indicating that AMX exposure may inhibit HMs passivation and promote some MRGs. The presence of AMX significantly altered bacterial community composition and AMX-resistant and -sensitive bacterial structures, elevating antibiotic resistome and its potential transmission risks, in which the proportions of ARB and intI1 were greatly increased to 148- and 11.6-fold compared to the control group. Proteobacteria and Actinobacteria were significant biomarkers of AMX exposure and may be critical in promoting bacterial resistance development. S0134_terrestrial_group was significantly negatively correlated with blaTEM and czrC genes, which might play a role in the elimination of some ARGs and MRGs. Except for the basic physicochemical (MC, C/N, and pH) and nutritional indicators (NO3−-N, NH4+-N), Bio-Cu may be an important environmental driver regulating bacterial resistance during composting. These findings suggested the importance of the interaction mechanism of combined pollution and its synergistic treatment during aerobic composting need to be emphasized.

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“…Pharmaceutical and personal care products (PPCPs) have received increased attention as emerging contaminants due to their pervasiveness and potentially undesirable ecological effects. , Due to the lack of proper recycling systems, most unused or expired PPCPs are discharged into municipal solid wastes (MSWs). , They were detected at high concentrations in MSW refuse samples. − For example, antibiotic concentrations in refuse samples ranged from 5 μg/kg to 1.4 mg/kg in China. , In most countries, landfilling has long been the main disposal method for MSWs. During the long-term landfilling process, PPCPs harbored in the refuse can be released and migrate to landfill leachates, leading to a PPCP concentration level of μg/L to mg/L in landfill leachates, orders of magnitude higher than other emission sources in water environments such as municipal wastewater. − …”

Section: Introductionmentioning

confidence: 99%

Rainfall Influences Occurrence of Pharmaceutical and Personal Care Products in Landfill Leachates: Evidence from Seasonal Variations and Extreme Rainfall Episodes

Sui

Lyu

et al. 2021

Environ. Sci. Technol.

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Unused or expired pharmaceutical and personal care products (PPCPs) are usually discharged into municipal solid wastes, then travel to landfills, and eventually percolate into leachates. However, knowledge of their occurrence and temporal dynamics in leachates is limited, making landfill leachate an underappreciated emission source of PPCPs. Furthermore, the differences in PPCP variations in landfill leachates emphasize the necessity for identifying the influencing factors and elucidating the mechanisms for PPCP fluctuations. In this study, successive monthly monitoring of PPCPs in leachates throughout an entire year was performed to determine their seasonal variations and identify their influencing factors. Furthermore, five pairs of additional sampling campaigns were conducted before and after rainfall events during wet seasons to elucidate the influencing mechanisms. The results showed that there was a distinct seasonal variation in PPCPs in landfill leachateselevated levels during the wet period (from April to September, with a mean concentration of 17.0 μg/L for total monitored PPCPs)when compared to other months (mean concentration of 3.8 μg/L). Rainfall played a considerable role in mediating PPCP concentrations in leachates. The PPCP responses to five rainfall episodes further verified the influence of rainfall and demonstrated that the tendency to PPCP concentration increase was related to rainfall precipitation. Torrential rain events (i.e., 24 h cumulative precipitation of 50–99.9 mm) led to the most significant increases in PPCP concentrations in landfill leachates. In addition, the hydrophilicity of PPCPs contributed to the different fluctuations during the 1 year investigation and different responses to rainfall. To the best of our knowledge, this study provides the first direct evidence supporting the influence of rainfall on PPCPs in landfill leachates, which can help better understand the occurrence and behavior of emerging contaminants in this underappreciated emission source.

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Prevalence of fluoroquinolone, macrolide and sulfonamide-related resistance genes in landfills from East China, mainly driven by MGEs

Cited by 43 publications

References 53 publications

Metagenomic characterization of bacterial community and antibiotic resistance genes in representative ready-to-eat food in southern China

Metagenomic characterization of bacterial community and antibiotic resistance genes in representative ready-to-eat food in southern China

Environmental drivers and interaction mechanisms of heavy metal and antibiotic resistome exposed to amoxicillin during aerobic composting

Rainfall Influences Occurrence of Pharmaceutical and Personal Care Products in Landfill Leachates: Evidence from Seasonal Variations and Extreme Rainfall Episodes

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