Prevalence of broad-spectrum cephalosporin-resistant Escherichia coli isolates in food samples in Tunisia, and characterization of integrons and antimicrobial resistance mechanisms implicated

Slama, Karim Ben; Jouini, Ahlem; Sallem, Rym Ben; Somalo, Sergio; Sáenz, Yolanda; Estepa, Vanesa; Boudabous, Abdellatif; Torres, Carmén

doi:10.1016/j.ijfoodmicro.2009.12.003

Cited by 67 publications

(58 citation statements)

References 44 publications

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“…This is consistent with the results presented by other investigators (2,23,32), and seems to be a consequence of an intensive use of antimicrobial agents in poultry farms. However, in some countries, e.g.…”

Section: Discussionsupporting

confidence: 93%

“…These data concerns more often the occurrence of the mentioned microorganisms in animals at the farm (3, 4) or the slaughter-house (5, 9), and in less or more processed meat or meat products (8,20,25). The investigations concerning raw meat were performed most often with poultry meat (2,6,28), and more rarely with meat of other animal species (23,32).…”

Section: Discussionmentioning

confidence: 99%

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Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Wasiński

Różańska

Osek

2013

Bulletin of the Veterinary Institute in Pulawy

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The aim of the study was a preliminary determination of occurrence of extended spectrum β-lactamases (ESBL)-and AmpC-producing Escherichia coli (E.coli) in raw meat samples collected from slaughter-houses located in different regions of Poland. A total of 141 samples were tested, comprising 78 pork samples, 44 beef samples, and 19 chicken meat samples. Isolated and identified E. coli strains were examined for the ESBL and/or AmpC β-lactamases production by the use of four disc diffusion and minimum inhibitory concentration tests. All strains positive in one or both tests were examined by PCR for the presence of the bla CTX , bla TEM , bla SHV , and bla CMY-2 group genes. During the study, 154 E. coli strains were isolated from 95 samples. Among these, 18 (11.7%) strains were identified in phenotypic tests as ESBL-producing and seven (4.5%) strains as AmpC-positive. The presence of the genes encoding selected ESBL-s (TEM, CTX, SHV) was identified in 14 of the strains recognised as ESBLpositive in phenotypic tests. All AmpC-positive isolates showed the presence of the CMY-2 group encoding genes. One of these strains had also the CTX-M and TEM genes, and four of them expressed the TEM marker.

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Section: Discussionsupporting

confidence: 93%

Section: Discussionmentioning

confidence: 99%

Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Wasiński

Różańska

Osek

2013

Bulletin of the Veterinary Institute in Pulawy

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“…The multiple antibiotic resistances of E. coli demonstrated in the current study is in agreement with other studies from various parts of the world (6,20,21).…”

Section: Discussionsupporting

confidence: 93%

“…E. coli can easily contaminate food products during animal evisceration at slaughter or during food manipulation (6). Drug resistant E. coli can easily spread through water, soil and food and can be transferred from animals to people (7).…”

Section: Introductionmentioning

confidence: 99%

Antibiotic Resistance Pattern of Escherichia coli Groups A, B1, B2 and D Isolated from Frozen Foods and Children with Diarrhea in Sanandaj, Iran

Darehabi¹,

Naseri²,

Menbari³

et al. 2013

Int J Enteric Pathog

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Background: Escherichia coli is usually a commensal of the digestive system, particularly intestine of humans and animals. However, there are many reports indicating that some strains are known to cause serious problems causing various infections among children worldwide, particularly in the developing countries. Escherichia coli strains can be classified into four phylogenetic groups: A, B1, B2 and D. The commensal strains are usually placed into the phylogenetic groups; A and B1 and the extraintestinal pathogenic strains into a group B2 and, to a lesser extent, group D. Objectives: The present study aimed to investigate the antibiotic resistance pattern of Escherichia coli groups A, B1, B2 and D from frozen foods and children with diarrhea. Materials and methods: From 125 frozen foods of animal origin and 466 rectal swabs from children with diarrhea, E. coli were isolated and identified based on standard procedures. Susceptibility testing to antibiotic was carried out according to the CLSI criteria. The phylogenetic group of each strain was determined by using multiplex PCR. Results: In the current study, 47 and 99 E. coli strains were isolated and allocated into four phylogenetic groups (i.e. A, B1, B2 and D). Among the strains isolated from the frozen foods of animal origin, 6.3% were allocated into phylogenetic group B2, and 23.4%% into D. Similarly, among the strains isolated from children with diarrhea 9% were allocated into group B2 and 19% to group D. The data showed that 55.3% and 53.1% of the frozen food-derived E. coli strains were resistant to Tetracycline and Amoxycillin respectively. Similarly, 89.9, 88.9 and 79.8% of isolates were resistant to tetracycline, chloramphenicol, and ampicillin, respectively. By a multiplex PCR procedure different phylogenetic E. coli were detected. Conclusion: Detection of E. coli isolates is very important and shows that food of animal origin can be of a reservoir for resistant bacteria that potentially could be transferred to humans through the food chain. In addition, results of the current study also revealed the detection of resistant E. coli isolates from children with diarrhea.

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“…Enterobacteriaceae are the significant causes of serious infection, and many of the most important members of this family are becoming increasingly resistant to currently available antimicrobials. Two organisms of concern are E. coli and Klebsiella pneumoniae, opportunistic pathogens of humans and animals are responsible for a wide range of infections, such as urinary tract infections, pneumonia, wound infections and septicemia (Slama et al, 2010). The present study was to evaluate the isolates of antibiotic resistant E. coli in clinical samples and their control.…”

Section: Introductionmentioning

confidence: 99%

Prevalence of Antibiotic Resistance in Clinical Strains of E. Coli and Their Control

Sujatha¹,

Anusuya²

2017

IOSRJPBS

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Prevalence of broad-spectrum cephalosporin-resistant Escherichia coli isolates in food samples in Tunisia, and characterization of integrons and antimicrobial resistance mechanisms implicated

Cited by 67 publications

References 44 publications

Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Occurrence of extended spectrum β-lactamaseand AmpC-producing Escherichia coli in meat samples

Antibiotic Resistance Pattern of Escherichia coli Groups A, B1, B2 and D Isolated from Frozen Foods and Children with Diarrhea in Sanandaj, Iran

Prevalence of Antibiotic Resistance in Clinical Strains of E. Coli and Their Control

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