Prevalence and resistance profile of extended-spectrum -lactamases-producing Enterobacteriaceae in Ouagadougou, Burkina Faso

Abstract

Increasing bacterial resistance to antimicrobial agents has become an issue of concern. A major problem of the treatment of infections caused by Enterobacteriaceae using antibiotics is the emergence of Extended-spectrum β-lactamases (ESBL)-producing Enterobacteriaceae. This study aims to determine the prevalence of ESBL-producing Enterobacteriaceae strains in Ouagadougou, Burkina Faso, and describe their resistance profile to other antibiotics commonly used in the infections treatment. 486 clinical strains of Enterobacteriaceae were obtained from patients attending three health centers in Ouagadougou (Burkina Faso) from November 2014 to October 2015. Biochemical identification was performed and antibiotics susceptibility test was performed using the disk diffusion method. Data was analyzed with the Excel and ANOVA one-way software GraphPad Prism version 5.01 software. Results revealed occurrence of Escherichia coli (60.9%, 194) predominated followed by Klebsiella spp. (22.4%, 109). Antibiotics susceptibility test revealed that 86.8% strains were resistant to amoxicillin, 81.3% to trimethoprim-sulfamethoxazole, 61.9% to ceftriaxone, 58.6% to cefotaxime and 58.4% to cefepime. It was observed that 99.8% were susceptible to imipenem while 16.6% were resistant to fosfomycin and 12.3% to amikacin. However, 38.5% (187/486) of the strains were ESBL-producing, 67.9% (127/187) of which came from Yalgado Ouedraogo University Hospital Center, 23.5% (44/187) from Charles De Gaulle Paediatric University Hospital Center and 8.6% (16/187) from Saint Camille Hospital. This study thus showed a high prevalence of Extended-Spectrum Β-Lactamases producing Enterobacteriaceae strains in Ouagadougou (38.5%). It underlined the need for routine detection and systematic reporting of ESBL strains in different health facilities in Burkina Faso, so that measures could be taken to prevent their spread and treatment failures.

Prevalence and resistance profile of <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> isolated from urinary tract infections in N'Djamena, Tchad

“…This practice could contribute to the selection of multi-resistant strains. The results of this study are comparable to those of Nadmi et al (2010), Ahmed et al (2012), Ndoutamia et al (2014), Sumera et al (2014) and Dissinviel et al (2017). Isolated strains in hospitalized patients were more resistant than those detected in outpatients.…”

Detection, identification and characterization of extended-spectrum beta-lactamases producing Enterobacteriaceae in wastewater and salads marketed in Ouagadougou, Burkina Faso

“…This predominance of the species Escherichia coli and Klebsilla pneumoniae in wastewater is linked to the fact that these two E-ESBLs are also the most isolated in digestive carriage in humans and animals (Ouédraogo et al, 2017;Sanou et al, 2019). In addition, these enterobacteria are the most isolated in infectious pathology in three hospitals in Burkina faso (Kpoda et al, 2017).…”

Molecular Characterization and the Antimicrobial Resistance Profile of Salmonella spp. Isolated from Ready-to-Eat Foods in Ouagadougou, Burkina Faso

“…Ampicillin, chloramphenicol, and trimethoprim-sulphamethoxazole constitute the first-line traditional antibiotics used for salmonellosis treatment [ 38 ]. High prevalence rates of resistance to these antibiotics have been described in Burkina Faso and elsewhere from other sources (e.g., humans and animals) [ 23 , 36 , 39 ]. Salmonella resistance to these affordable antimicrobials is a public health threat.…”