Prevalence and relationship of human papilloma virus type 16 and type 18 with oral squamous cell carcinoma and oral leukoplakia in fresh scrappings: A PCR study

Mathew, Asok; Mody, RN; Patait, Mahendra; Razooki, Ali A; Varghese, Nisha T; Saraf, Kedar

doi:10.4103/0019-5359.106612

Cited by 17 publications

(10 citation statements)

References 4 publications

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“…Various studies analysed the impact of HPV infection on head and neck cancer with HPV positivity in 24%–73% of the investigated tumours (Jalouli et al., ; Mathew et al., ; Ostwald et al., ; Prakash et al., ). Although the role of HPV infections in the pathogenesis of oropharyngeal cancers is proven, the impact of oral HPV infection on development of OSCC remains unclear.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Human papillomavirus prevalence in a Mid‐European oral squamous cell cancer population: A cohort study

Torre¹,

Burtscher

Soelder

et al. 2018

Oral Diseases

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Section: Discussionmentioning

confidence: 99%

“…Various studies analysed the impact of HPV infection on head and neck cancer with HPV positivity in 24%-73% of the investigated tumours (Jalouli et al, 2010;Mathew et al, 2011;Ostwald et al, 2003;Prakash et al, 2013). Although the role of HPV infections in could be shown, which was previously described mainly for OPSCC (Fakhry et al, 2008).…”

Section: Discussionmentioning

confidence: 99%

Human papillomavirus prevalence in a Mid‐European oral squamous cell cancer population: A cohort study

Torre¹,

Burtscher

Soelder

et al. 2018

Oral Diseases

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“…HPV18 is the second most common HPV type found in cervical cancers [2, 18] and several studies have reported that HPV18 may have an equal or greater prevalence than HPV16 in HPV-associated oral cancer cases in countries including Brazil [20], the United Arab Emirates [21], Iran [22], and Taiwan [23]. We have previously generated two prototype therapeutic HPV DNA vaccines targeting HPV16-E6, the pcDNA3-HPV16-E6 (encoding wild type HPV16-E6 protein) and the pcDNA3-CRT/HPV16-E6 (encoding a fusion protein of HPV16-E6 with calreticulin) and evaluated the immunogenicity and antitumor effects of these vaccines in C57BL/6 mice [24].…”

Section: Discussionmentioning

confidence: 99%

Characterization of HPV18 E6-specific T cell responses and establishment of HPV18 E6-expressing tumor model

Yang

Peng

et al. 2017

Vaccine

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Human papillomavirus (HPV) has been identified as the primary etiologic factor of cervical cancer, and subsets of anogenital and oropharyngeal cancers. HPV18 is the second most prevalent high-risk HPV type after HPV16. Furthermore, HPV18 is responsible for approximately 12% of cervical squamous cell carcinoma and 37% of cervical adenocarcinoma cases worldwide. In this study, we aimed to characterize the HPV18-E6-specific epitope and establish an HPV18 animal tumor model to evaluate the E6-specific immune response induced by our DNA vaccine. We vaccinated naïve C57BL/6 mice with a prototype DNA vaccine, pcDNA3-HPV18-E6, via intramuscular injection followed by electroporation, and analyzed the E6-specific CD8+ T cell responses by flow cytometry using a reported T cell epitope. We then characterized the MHC restriction element for the characterized HPV18-E6 epitope. Additionally, we generated an HPV18-E6-expressing tumor cell line to study the antitumor effect mediated by E6-specific immunity. We observed a robust HPV18-E6aa67-75 peptide-specific CD8+ T cell response after vaccination with pcDNA3-HPV18-E6. Further characterization demonstrated that this epitope was mainly restricted by H-2Kb, but was also weakly presented by HLA-A*0201, as previously reported. We observed that vaccination with pcDNA3-HPV18-E6 significantly inhibited the growth of HPV18-E6-expressing tumor cells, TC-1/HPV18-E6, in mice. An antibody depletion study demonstrated that both CD4+ and CD8+ T cells are necessary for the observed antitumor immunity. The characterization of HPV18-E6-specific T cell responses and the establishment of HPV18-E6-expressing tumor cell line provide infrastructures for further development of HPV18-E6 targeted immunotherapy.

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“…Recent studies have found that the frequency of HPV-16 infection in OSCC ranges from 10.6 to 81.3 % using PCR 3,[23][24][25][26][27][28] . In this study, HPV-16 positivity rates among all PT specimens were 39.2 % (51/130), which is within the range reported in the above-mentioned study.…”

Section: Discussionmentioning

confidence: 99%

Human Papillomavirus Type 16 Infection and Local Lymph Node Metastasis in Patients with Squamous Cell Carcinoma

Kawai

Isomura

Sato

et al. 2016

J. Hard Tissue Biology.

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Human papillomavirus (HPV) infection is known to be an independent etiologic factor for oral squamous cell carcinoma (OSCC). Especially, HPV-16 is associated with a significant risk of developing OSCC. The most important prognostic factor in OSCC is local lymph node metastasis (LNM); therefore, knowledge of LNM status is crucial for selecting proper treatment plans. However, it is not clarify relationship between HPV-16 infection and LNM in OSCC. The purpose of this study was to determine the role of HPV-16 infection in LNM in OSCC. We analyzed 130 cases of OSCC (100 cases of OSCC without LNM; 30 cases of OSCC with LNM). HPV-16 infection was detected by PCR, immunohistochemical examination and in situ hybridization. HPV-16 positivity rates among primary tumor (PT) specimens without LNM were 43.0 % (43/100), and HPV-16 positivity rates among PT specimens with LNM were 26.7 % (8/30). In addition, HPV-16 positivity rates in both PT and LNM specimens in 30 OSCC patients with LNM were 10 % (3/30). OSCC with HPV-16 DNA detected by PCR showed positive staining on immunohistochemical examination and in situ hybridization. The HPV-16 infection rate in OSCC with LNM was significantly lower than that for OSCC without LNM. In the case of OSCC with LNM, HPV-16 infection rates for both in PT and LNM were low. This suggests that HPV-16 positive cases had a significantly lower risk of LNM when compared with patients having HPV-16 negative OSCC. The results of the present study suggest that HPV status in OSCC is able to act as a marker for risk of LNM.

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Prevalence and relationship of human papilloma virus type 16 and type 18 with oral squamous cell carcinoma and oral leukoplakia in fresh scrappings: A PCR study

Cited by 17 publications

References 4 publications

Human papillomavirus prevalence in a Mid‐European oral squamous cell cancer population: A cohort study

Human papillomavirus prevalence in a Mid‐European oral squamous cell cancer population: A cohort study

Characterization of HPV18 E6-specific T cell responses and establishment of HPV18 E6-expressing tumor model

Human Papillomavirus Type 16 Infection and Local Lymph Node Metastasis in Patients with Squamous Cell Carcinoma

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