Prevalence and molecular characterization of enteropathogenic Escherichia coli isolated from table eggs in Mansoura, Egypt

Elafify, Mahmoud; Elsherbini, Mohammed; Abdel-Khalek, A. E.; Al-Ashmawy, Maha

doi:10.5455/javar.2016.c123

Cited by 6 publications

(5 citation statements)

References 24 publications

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“…Colony growth character on different media exhibited characteristics reaction. In this study, colony characteristics of E. coli observed on EMB and MC agars were similar to the findings of other authors (Rehman et al, 2014;Hassan et al, 2014;Mamun et al, 2016;Tanzin et al, 2016;Elafify et al, 2016;Islam et al, 2016).…”

Section: Resultssupporting

confidence: 79%

“…These findings were in support of the findings of Hassan et al (2014), Tanzin et al (2016), Elafify et al (2016) and Islam et al (2016).…”

Section: Identification Of E Coli By Gram Stainingsupporting

confidence: 82%

“…This indicated the isolates were E. coli. The isolates also revealed positive reaction in MR test and Indole test but negative reaction in VP test, which was supported by several authors (Hassan et al, 2014;Mamun et al, 2016;Tanzin et al, 2016;Elafify et al, 2016;Islam et al, 2016).…”

Section: Identification Of E Coli By Biochemical Testssupporting

confidence: 62%

“…Fecal isolates revealed a complete fermentation of basic sugars as stated by Beutin et al (1993), Sandhu et al (1996) and Mckec et al (1995). E. coli isolates were able to ferment the five basic sugars producing both acid and gas; however, differentiation of E. coli into species level was difficult as showed similar reaction in various sugars (Elafify et al, 2016). All the isolates fermented dextrose, sucrose, fructose, maltose and mannitol with the production of acid and gas within 24 h of incubation.…”

Section: Identification Of E Coli By Biochemical Testsmentioning

confidence: 99%

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Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

Akter

Majumder

Nazir

et al. 2016

J Bangladesh Agric Univ

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Shiga toxin-producing Escherichia coli (STEC) are zoonotically important pathogen which causes hemorrhagic colitis, diarrhea, and hemolytic uremic syndrome in animals and humans. The present study was designed to isolate and identify the STEC from fecal samples of diarrheic cattle. A total of 35 diarrheic fecal samples were collected from Bangladesh Agricultural University (BAU) Veterinary Teaching Hospital. The samples were primarily examined for the detection of E. coli by cultural, morphological and biochemical characteristics, followed by confirmation of the isolates by Polymerase Chain Reaction (PCR) using gene specific primers. Later, the STEC were identified among the isolated E. coli through detection of Stx-1 and Stx-2 genes using duplex PCR. Out of 35 samples, 25 (71.43%) isolates were confirmed to be associated with E. coli, of which only 7 (28%) isolates were shiga toxin producers, and all of them were positive for Stx-1. However, no Stx-2 positive isolate could be detected. From this study, it may be concluded that cattle can act as a reservoir of STEC which may transmit to human or other animals.

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Section: Resultssupporting

confidence: 79%

“…These findings were in support of the findings of Hassan et al (2014), Tanzin et al (2016), Elafify et al (2016) and Islam et al (2016).…”

Section: Identification Of E Coli By Gram Stainingsupporting

confidence: 82%

Section: Identification Of E Coli By Biochemical Testssupporting

confidence: 62%

Section: Identification Of E Coli By Biochemical Testsmentioning

confidence: 99%

See 2 more Smart Citations

Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

Akter

Majumder

Nazir

et al. 2016

J Bangladesh Agric Univ

View full text Add to dashboard Cite

show abstract

“…Then samples from the nutrient broth were cultured on Eosin Methylene Blue (EMB) agar medium (Zadik et al, 1993). One colony from each EMB plate that showed metallic sheen on EMB agar was subjected to sugar (dextrose, maltose, lactose, sucrose, and mannitol) fermentation, Methyl Red-Voges Proskauer (MR-VP) and indole production tests following the procedures described by Nazir et al (2005) and Elafify et al (2016).…”

Section: Cultural and Biochemical Examinationmentioning

confidence: 99%

Prevalence and Characterization of Shigatoxigenic Escherichia Coli in Broiler Birds in Mymensingh

Mamun¹,

Parvej²,

Ahamed³

et al. 2016

Bangl. J. Vet. Med.

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Shigatoxigenic Escherichia coli (STEC) are major food-borne pathogens. They transmit to human through contaminated meat and meat products of animals and poultry, and frequently associated with various types of human illness including haemolytic uremic syndrome. This preliminary study showed the prevalence of STEC in 60 cloacal swab samples of live healthy broiler chickens collected randomly when sold at a wholesale market in Mymensingh district of Bangladesh. Isolation and identification of E. coli was carried out using Eosin Methylene Blue (EMB) agar media and 16S rRNA gene specific polymerase chain reaction (PCR). Among the 60 samples, 49 (81.67%) were found positive to E. coli. These E. coli isolates were screened for the detection of STEC by PCR using stx1 and stx2 gene specific primers. Among the 49 positive samples, 5 (10.20%) were found positive for stx1 gene, and 26 (53.06%) were positive for stx2 gene. In addition, 6 (12.24%) isolates were found positive to both stx1 and stx2 genes, and the remaining 12 (22.46%) were negative. The high prevalence ofSTEC in the broiler chicken alarms the public health impact as the people are always in close contact with these live broiler chickens in the open market as well as processing of meat at home before cooking. However, further studies are required to uncover the major source(s) for the transmission of STEC to human in ruralBangladesh.

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The study and manipulation of spermatogonial stem cells using animal models

2020

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Prevalence and molecular characterization of enteropathogenic Escherichia coli isolated from table eggs in Mansoura, Egypt

Cited by 6 publications

References 24 publications

Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

Prevalence and molecular detection of shiga toxin producing Escherichia coli from diarrheic cattle

Prevalence and Characterization of Shigatoxigenic Escherichia Coli in Broiler Birds in Mymensingh

The study and manipulation of spermatogonial stem cells using animal models

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