Prevalence and Clonal Diversity of over 1,200 Listeria monocytogenes Isolates Collected from Public Access Waters near Produce Production Areas on the Central California Coast during 2011 to 2016

Gorski, Lisa; Cooley, Michael B.; Oryang, David; Carychao, Diana; Nguyen, Kimberly; Luo, Yan; Weinstein, Leah; Brown, Eric W.; Allard, Marc W.; Mandrell, Robert E.; Chen, Yi

doi:10.1128/aem.00357-22

Cited by 35 publications

(27 citation statements)

References 113 publications

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“…Notably, none of the 218 isolates from natural environments contained biocide resistance genes qacH , bcrABC , or inlA PMSC mutations. This concurs with a recent study of L. monocytogenes collected from surface waters in California ( 83 ), in which qacH and bcrABC were detected in 0 and 18 isolates, respectively, while inlA PMSC mutations were present in only four of 1,248 examined isolates. In contrast, we identified qacH or bcrABC genes in 45% and inlA PMSC mutations in 51% of isolates from food processing environments.…”

Section: Discussionsupporting

confidence: 93%

Pervasive Listeria monocytogenes Is Common in the Norwegian Food System and Is Associated with Increased Prevalence of Stress Survival and Resistance Determinants

Fagerlund

Wagner

Møretrø

et al. 2022

Appl Environ Microbiol

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Section: Discussionsupporting

confidence: 93%

Pervasive Listeria monocytogenes Is Common in the Norwegian Food System and Is Associated with Increased Prevalence of Stress Survival and Resistance Determinants

Fagerlund

Wagner

Møretrø

et al. 2022

Appl Environ Microbiol

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show abstract

“…Notably, none of the 218 isolates from natural environments contained biocide resistance genes qacH , bcrABC , or inlA PMSC mutations. This concurs with a recent study of L. monocytogenes collected from surface waters in California (83), in which qacH and bcrABC were detected in zero and 18 isolates respectively, while inlA PMSC mutations were present in only four of 1248 examined isolates. In contrast, we identified qacH or bcrABC genes in 45% and inlA PMSC mutations in 51% of isolates from food processing environments.…”

Section: Discussionsupporting

confidence: 93%

PervasiveListeria monocytogenesare common in the Norwegian food system and associated with increased prevalence of stress survival and resistance determinants

Fagerlund

Wagner

Møretrø

et al. 2022

Preprint

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To investigate the diversity, distribution, persistence, and stress resistome of Listeria monocytogenes clones dominating in food processing environments in Norway, genome sequences from 769 L. monocytogenes isolates from food industry environments, foods, and raw materials (of which 512 were sequenced in the present study) were subjected to wgMLST, SNP, and comparative genomic analyses. The dataset comprised isolates from nine meat and six salmon processing facilities in Norway collected over a period of three decades. The most prevalent clonal complex (CC) was CC121, found in ten factories, followed by CC7, CC8, and CC9, found in seven factories each. Overall, 72% of the isolates were classified as persistent, showing 20 or fewer wgMLST allelic differences towards an isolate found in the same factory in a different calendar year. Moreover, over half of the isolates (56%) showed this level of genetic similarity towards an isolate collected from a different food processing facility. These were designated as pervasive strains, defined as clusters with the same level of genetic similarity as persistent strains but isolated from different factories. The prevalence of genetic determinants associated with increased survival in food processing environments, including heavy metal and biocide resistance determinants, stress response genes and inlA truncation mutations, showed a highly significant increase among pervasive isolates, but not among persistent isolates. Furthermore, these genes were significantly more prevalent among the isolates from food processing environments compared to in isolates from natural and rural environments (n=218) and clinical isolates (n=111) from Norway.

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“…Although not presenting a Premature Stop Codon Mutation in the inlA gene nor the SSI-1, both associated with an increased production of biofilm, as reported in previous studies ( Keeney et al, 2018 ; Upham et al, 2019 ; Mahoney et al, 2022 ), all the strains carried several genetic markers for biofilm production. In particular luxS , for a S-ribosylhomocysteinase, recO encoding the DNA repair protein RecO and lmo2504 for a cell wall-binding protein were found in all the genomes ( Pasquali et al, 2018 ; Gorski et al, 2022 ). The lmo0673 gene, encoding an hypothetical protein and reported as marker of biofilm production by Pasquali et al (2018) , was also found.…”

Section: Discussionmentioning

confidence: 99%

Atypical Serogroup IVb-v1 of Listeria monocytogenes Assigned to New ST2801, Widely Spread and Persistent in the Environment of a Pork-Meat Producing Plant of Central Italy

et al. 2022

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In this study, we characterized 84 Listeria monocytogenes (Lm) strains having an atypical IVb-v1 profile and isolated in a meat producing plant of Central Italy. They were assigned to the new MLST type ST2801 (CC218). The new ST was widespread in the food-producing environment where it was able to persist for over a year even after cleaning and sanitation. Cluster analysis identified three main clusters genetically close to each other (0–22 allelic differences and 0–28 SNPs) from two different cgMLST types, suggesting a common source. The coexistence of closely related clusters over time could be the result of a different evolution path starting from a common ancestor first introduced in the plant and/or the consequence of the repetitive reintroduction of closely related clones probably by raw materials. All the strains presented several determinants for heavy metals resistance, stress response, biofilm production, and multidrug efflux pumps with no significant differences among the clusters. A total of 53 strains carried pLI100 and the j1776 plasmids, while in one strain, the pLM33 was found in addition to pLI100. Only the strains carrying plasmids presented cadA and cadC for cadmium resistance and the mco gene encoding a multicopper oxidase and gerN for an additional Na+/H+-K+ antiporter. All the strains presented a virulence profile including a full-length inlA gene and the additional LIPI-3. The isolation of a new ST with a large pattern of stress-adaptation genes and able to persist is an important contribution to deepening the current knowledge on the uncommon IVb-v1 and in general on the genomic diversity of Lm.

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Prevalence and Clonal Diversity of over 1,200 Listeria monocytogenes Isolates Collected from Public Access Waters near Produce Production Areas on the Central California Coast during 2011 to 2016

Cited by 35 publications

References 113 publications

Pervasive Listeria monocytogenes Is Common in the Norwegian Food System and Is Associated with Increased Prevalence of Stress Survival and Resistance Determinants

Pervasive Listeria monocytogenes Is Common in the Norwegian Food System and Is Associated with Increased Prevalence of Stress Survival and Resistance Determinants

PervasiveListeria monocytogenesare common in the Norwegian food system and associated with increased prevalence of stress survival and resistance determinants

Atypical Serogroup IVb-v1 of Listeria monocytogenes Assigned to New ST2801, Widely Spread and Persistent in the Environment of a Pork-Meat Producing Plant of Central Italy

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