Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types

Menandro, Maria Luisa; Dotto, Giorgia; Mondin, Alessandra; Martini, Marco; Ceglie, Letizia; Pasotto, Daniela

doi:10.1016/j.cimid.2019.101331

Cited by 32 publications

(24 citation statements)

References 33 publications

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“…Staphylococcus pseudintermedius (S. pseudintermedius), an emerging zoonotic agent of canine origin, is an important opportunistic pathogen causing various infections in dogs, such as otitis externa, pyoderma, and wound infections [1][2][3]. Multidrug-resistant (MDR) methicillin-resistant S. pseudintermedius (MRSP) and methicillin-susceptible S. pseudintermedius (MSSP) strains represent an important problem in animal health and an increasing therapeutic challenge in veterinary medicine [4].…”

Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of the Iron-Chelator, DIBI, against Multidrug-Resistant Canine Methicillin-Susceptible Staphylococcus pseudintermedius: A Preliminary Study of Four Clinical Strains

et al. 2022

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Staphylococcus pseudintermedius is an important opportunistic pathogen causing various infections in dogs. Furthermore, it is an emerging zoonotic agent and both multidrug-resistant methicillin-resistant S. pseudintermedius (MRSP) as well as methicillin-susceptible (MSSP) strains represent an important therapeutic challenge to veterinary medicine and pose a potential threat to human health. We tested representative S. pseudintermedius clinical strains from dogs suffering from otitis externa for their susceptibilities to a panel of 17 antimicrobials compared to DIBI. DIBI, unlike antibiotics, is a novel water-soluble hydroxypyridinone-containing iron-chelating agent that deprives microbes of growth-essential iron and has been previously shown to inhibit methicillin-resistant Staphylococcus aureus (MRSA). We also characterised the strains according to whether they harbour key antibiotic resistance genes. The strains each displayed multiple antimicrobial resistance patterns; all were negative for the mecA gene and possessed the tetK and tetM genes, but they varied as to their possession of the ermB gene. However, all the isolates had similar susceptibility to DIBI with low MICs (2 µg/mL or 0.2 µM). Because the four MSSPs were equally susceptible to DIBI, subject to confirmation with additional strains, this could provide a potential non-antibiotic, anti-infective alternative approach for the treatment of antimicrobial-resistant canine S. pseudintermedius otitis.

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Section: Introductionmentioning

confidence: 99%

Antimicrobial Activity of the Iron-Chelator, DIBI, against Multidrug-Resistant Canine Methicillin-Susceptible Staphylococcus pseudintermedius: A Preliminary Study of Four Clinical Strains

et al. 2022

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“…Staphylococcal infections in domestic companion animals, especially in dogs and cats, can commonly cause superficial pyoderma, otitis externa, superficial bacterial folliculitis, and bacterial rhinitis. The main cause of these infections are often S. pseudintermedius [ 10 , 23 , 24 ]. The S. intermedius group (SIG) accounted for the majority of the isolates recovered from clinical pyoderma cases in this study.…”

Section: Discussionmentioning

confidence: 99%

The SCCmec Types and Antimicrobial Resistance among Methicillin-Resistant Staphylococcus Species Isolated from Dogs with Superficial Pyoderma

Chanayat

Akatvipat

Bender

et al. 2021

Veterinary Sciences

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This study characterizes clinical methicillin-resistant staphylococcal (MRS) isolates obtained from superficial pyoderma infections in dogs. Our interest was to determine the staphylococcal cassette chromosome mec (SCCmec) type and the antimicrobial susceptibility among MRS isolates from clinical cases. Skin swabs were collected and cultured. Staphylococcus species were identified and characterized with biochemical tests and MALDI-TOF-MS and antimicrobial susceptibility testing by disk diffusion. mecA detection and staphylococcal cassette chromosome mec (SCCmec) typing were achieved by PCR. Of the 65 clinical samples, 56 (86.2%) staphylococcal infections were identified. Twelve (21%) of 56 isolates were MRS infections. All MRS isolates were multidrug resistant. The ccrC and class-C2 mec, which were SCCmec type V, were the most prevalent (66.7%) among the 12 MRS isolates. The predominant SCCmec type V was found in S. aureus, S. intermedius group, S. lentus, S. xylosus, and S. arlettae. Treatment failure is a concern with the emergence of highly resistant MRS in dogs associated with superficial pyoderma. The detection of type V SCCmec MRS has previously been reported among veterinarians and dog owners but not in Northern Thailand. These infections serve as a reminder to improve infection prevention and control measures including reducing environmental contamination and potential zoonotic exposures to MRS. In addition, educational awareness of these risks in small animal hospitals needs to be increased among veterinary hospital staff, clients, and patients.

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“…S . pseudintermedius is also a cause of otitis and urinary tract infections in dogs [ 16 , 17 ]. In a large case series from Italy [ 16 ], S .…”

Section: What Are the Clinical Manifestations Of S Pseudintermedius In Animals And Humans?mentioning

confidence: 99%

“…pseudintermedius is also a cause of otitis and urinary tract infections in dogs [ 16 , 17 ]. In a large case series from Italy [ 16 ], S . pseudintermedius constituted 76% of all Staphylococcus species recovered from dog and cat cultures, and 31.6% of those were MRSP.…”

Section: What Are the Clinical Manifestations Of S Pseudintermedius In Animals And Humans?mentioning

confidence: 99%

From canines to humans: Clinical importance of Staphylococcus pseudintermedius

2021

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Prevalence and characterization of methicillin-resistant Staphylococcus pseudintermedius from symptomatic companion animals in Northern Italy: Clonal diversity and novel sequence types

Cited by 32 publications

References 33 publications

Antimicrobial Activity of the Iron-Chelator, DIBI, against Multidrug-Resistant Canine Methicillin-Susceptible Staphylococcus pseudintermedius: A Preliminary Study of Four Clinical Strains

Antimicrobial Activity of the Iron-Chelator, DIBI, against Multidrug-Resistant Canine Methicillin-Susceptible Staphylococcus pseudintermedius: A Preliminary Study of Four Clinical Strains

The SCCmec Types and Antimicrobial Resistance among Methicillin-Resistant Staphylococcus Species Isolated from Dogs with Superficial Pyoderma

From canines to humans: Clinical importance of Staphylococcus pseudintermedius

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