Prevalence and Characterization of Extended-Spectrum Beta-Lactamase (ESBL)– and CMY-2–Producing<i>Escherichia coli</i>Isolates from Healthy Food-Producing Animals in Tunisia

Sallem, Rym Ben; Slama, Karim Ben; Sáenz, Yolanda; Rojo–Bezares, Beatriz; Estepa, Vanesa; Jouini, Ahlem; Gharsa, Haythem; Klibi, Naouel; Boudabous, Abdellatif; Torres, Carmén

doi:10.1089/fpd.2012.1267

Cited by 73 publications

(69 citation statements)

References 38 publications

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“…In the Netherlands, Overdevest et al (2011) and Leversteinvan Hall et al (2011) reported 80 and 94% prevalence of ESBLs-producing E. coli in chicken meat and raw meat, respectively. Escudero et al (2010), Schmid et al (2013), Adenaike et al (2013), Ben Sallem et al (2012) and Guandong and Avci (2013) reported 72, 21.4, 45, 13.8 and 44.4% prevalence of ESBL-producing E. coli from pigs in Spain, beef cattle in Germany, roasted beef in Nigeria, foodproducing animals in Tunisia and food of animal origin in Turkey, respectively. The isolation prevalence of ESBLresistant E. coli (8.7%) in this study is lower when compared with those of the previous studies.…”

Section: Discussionmentioning

confidence: 98%

“…AmpC cephalosporinases mediate resistance to 3 rd -generation β-lactams/cephalosporins, but are not inhibited by clavulanic acid and other β-lactamase inhibitors (such as sulbactam and tazobactam) (Ben Sallem et al, 2012). Conversely, the ESBLs are inhibited by clavulanic acid and other β-lactamase inhibitors (GarciaGraells et al, 2010;Ben Sallem et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

“…This has led to increased resistance to most of the antibacterial agents (especially the β-lactams) (CLSI, 2012). Enterobacteriaceae constitute normal bacterial flora in intestinal tract of foodproducing animals and humans (Ben Sallem et al, 2012). Indiscriminate use of β-lactams (particularly 3rd-[e.g.…”

Section: Introductionmentioning

confidence: 99%

“…With the current global target of "one world, one health", efforts are being intensified to detect sources of antibacterial resistance (especially β-lactam resistance) in order to curb its spread in both humans and animals (Wall, 2014). Surveillance studies to assess foodproducing animals as potential reservoirs and disseminators of ESBL-resistant bacteria have been conducted in several countries in Europe (Aestrup et al, 2006;Meunier et al, 2006;Cloeckaert et al, 2007;Girlich et al, 2007;Smet et al, 2008;Wu et al, 2008;Cortes et al, 2010;Goncalves et al, 2010;Geser et al, 2011;Geser et al 2012;Hammerrum et al, 2014), Asia (Duan et al, 2006;Tian et al, 2009;Yuan et al, 2009;Li et al, 2014;Wang et al, 2014), Africa (Jouini et al, 2007;Ben Sallem et al, 2012), North America (Wittum et al, 2010) and South America (Fernandes et al, 2009). Unfortunately, there is paucity of information on ESBLresistant bacteria in food-producing animals in Nigeria.…”

Section: Introductionmentioning

confidence: 99%

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Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

et al. 2015

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This study was conducted to isolate generic extended-spectrum β-lactam (ESBL)-resistant enterobacteria from pigs reared in Enugu State Southeast, Nigeria and determine the antibacterial resistance profile of the isolates. Rectal swabs were collected from 190, randomly selected, apparently healthy pigs. Isolation of ESBL-resistant enterobacteria was done using Mac Conkey agar supplemented with 2 µg/ml of cefotaxime. Phenotypic characterization of the isolates to generic level was done following standard biochemical methods. Phenotypic resistance of the isolates to antibacterial agents was determined using the disc diffusion method. Out of 46 ESBL-resistant enterobacterial isolates, 4 (8.7%) were Escherichia coli, 11 (23.9%) were Salmonella species, while 31 (67.4%) were Klebsiella species. Resistance of the Salmonella isolates was 45.5% to ciprofloxacin, 36.4% to ofloxacin and levofloxacin, 9.1% to norfloxacin, amikacin and gentamicin, 27.3% to streptomycin, 72.7% to chloramphenicol and 90.9% to tetracycline. Resistance of the Klebsiella isolates was 93.5% to ampicillin, 12.9% to ciprofloxacin, 19.4% to ofloxacin and levofloxacin, 9.7% to norfloxacin and streptomycin, 64.5% to chloramphenicol and 38.7% to tetracycline. Resistance of the E. coli isolates was 100% to gentamicin, 75% to ampicillin and streptomycin, 50% to ciprofloxacin, norfloxacin, chloramphenicol and tetracycline, and 25% to ofloxacin, levofloxacin and amikacin. All the isolates were resistant to ceftriaxone, cefotaxime, ceftazidime, cefepime, cefpodoxime, amoxicillin/clavulanic acid and aztreonam. Resistance of the isolates to more than 3 classes of antibacterial agents tested was 54.8% for Klebsiella, 90.9% for Salmonella and 100% for E. coli, respectively. This study has shown that pigs reared in Enugu State Southeast, Nigeria, are colonized by ESBL-resistant Enterobactericeae and are potential reservoirs and disseminators of these organisms.

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Section: Discussionmentioning

confidence: 98%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

et al. 2015

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“…coli isolated from animals with multiple antibiotic-resistant phenotypes have been reported in Tunisia and worldwide [13,14]. This situation has resulted in a need for more epidemiological information on the prevalence of resistance to various antibiotics and their relevant genes, such as virulence gene combinations in animal isolates.…”

Section: Introductionmentioning

confidence: 99%

Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia

Kilani

Abbassi

Ferjani

et al. 2017

J Infect Dev Ctries

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Introduction: The virulent Escherichia coli strains responsible for extraintestinal infections were mainly belonged to B2 and D phylogroups. However, no past studies have determinate via the presence of virulence genes the frequency of E. coli pathovars recovered from animals housed in farms in Tunisia. The aims of this study were to investigate 26 E. coli isolated from healthy and diarrheic animals and to determinate via the presence of virulence genes the frequency of pathovars. Methodology: Twenty-six E. coli isolates of phylogroups B2 (n = 14), B22 (n = 9), B23 (n = 5), and D2 (n = 12) were characterized. Genes encoding virulence factors (fimH,eaeA,aggC,papC, papG allele III, hlyA, east1, cnf1, exhA,stx1, stx2, iutA, fyuA, ibeA,and ipaH), and antibiotic resistance as well as class 1 and 2 integrons were searched by polymerase chain reaction (PCR). The genetic relationship of isolates was done by PFGE. Results: According to the occurrence of specific genes the 26 isolates were classified as:9 EAEC, 2 EHEC, 4 UPEC, 3 EPEC/EHEC and 1 NTEC. Therefore, 2 Ex-PEC and 5 APEC were presented amongst our strains. Some isolates (12) were clonal and the remaining was unrelated. Conclusions: Higher diversity of pathovars which carried diverse combinations of virulence genes in healthy isolates. In addition, it seems that the infections were caused by different mechanisms.

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Antimicrobial resistance patterns and characterisation of emerging beta‐lactamase‐producing Escherichia coli in camels sampled from Northern Kenya

Akunda

Kariuki

Matulis

et al. 2023

Veterinary Medicine & Sci

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Background: Animal husbandry practices in different livestock production systems and increased livestock-wildlife interactions are thought to be primary drivers of antimicrobial resistance (AMR) in Arid and Semi-Arid Lands (ASALs). Despite a tenfold increase in the camel population within the last decade, paired with widespread use of camel products, there is a lack of comprehensive information concerning beta-lactamase-producing Escherichia coli (E. coli) within these production systems.Objectives: Our study sought to establish an AMR profile and to identify and characterise emerging beta-lactamase-producing E. coli isolated from faecal samples obtained from camel herds in Northern Kenya. Methods:The antimicrobial susceptibility profiles of E. coli isolates were established using the disk diffusion method, with beta-lactamase (bla) gene PCR product sequencing performed for phylogenetic grouping and genetic diversity assessments.Results: Here we show, among the recovered E. coli isolates (n = 123), the highest level of resistance was observed for cefaclor at 28.5% of isolates, followed by cefotaxime at 16.3% and ampicillin at 9.7%. Moreover, extended-spectrum beta-lactamase (ESBL)producing E. coli harbouring the bla CTX-M-15 or bla CTX-M-27 genes were detected in 3.3% of total samples, and are associated with phylogenetic groups B1, B2 and D. Multiple variants of non-ESBL bla TEM genes were detected, the majority of which were the bla TEM-1 and bla TEM-116 genes.Conclusions: Findings from this study shed light on the increased occurrence of ESBLand non-ESBL-encoding gene variants in E. coli isolates with demonstrated multidrug resistant phenotypes. This study highlights the need for an expanded One Health approach to understanding AMR transmission dynamics, drivers of AMR development, and appropriate practices for antimicrobial stewardship in camel production systems within ASALs.

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Prevalence and Characterization of Extended-Spectrum Beta-Lactamase (ESBL)– and CMY-2–ProducingEscherichia coliIsolates from Healthy Food-Producing Animals in Tunisia

Cited by 73 publications

References 38 publications

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

Prevalence and Antibiogram of Generic Extended-Spectrum β-Lactam-Resistant Enterobacteria in Healthy Pigs

Diverse Escherichia coli pathovars of phylogroups B2 and D isolated from animals in Tunisia

Antimicrobial resistance patterns and characterisation of emerging beta‐lactamase‐producing Escherichia coli in camels sampled from Northern Kenya

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