Prevalence and characterisation of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in healthy volunteers in Tunisia

Sallem, Rym Ben; Slama, Karim Ben; Estepa, Vanesa; Jouini, Ahlem; Gharsa, Haythem; Klibi, Naouel; Sáenz, Yolanda; Ruiz-Larrea, Fernanda; Boudabous, A.; Torres, Carmén

doi:10.1007/s10096-011-1471-z

Cited by 88 publications

(69 citation statements)

References 27 publications

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“…In our study, the prevalence of ESBL-E. coli carriage in stool was as high as 66.3%. This is much higher than in a 2012 study in northern Cameroon which reported a prevalence of 23.1% among community outpatients [3] or in other African studies which reported rates of 30.9 % in Niger [10], 10% in Senegal [11] and 7.3 % in Tunisia [12]. This is even higher to what has been reported in China where a rate of 50.5% of CTX-M-E. coli [13] was described.…”

Section: Discussioncontrasting

confidence: 56%

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Djuikoue

Woerther

Toukam

et al. 2016

J Infect Dev Ctries

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Introduction: During the last decade, the prevalence of the intestinal carriage of extended spectrum beta-lactamases -producing Escherichia coli (ESBL-E. coli) has continued to increase worldwide in the community, especially in developing countries. Hence, we undertook a study to determine the ESBL-E. coli fecal carriage rate and the associated risk factors in Cameroonian women. Methodology: A total of 86 women suspected of community-acquired urinary tract infections (UTI) were included in 10 health structures from May 2011 to April 2012. After filling a questionnaire, they provided a stool sample that was plated on selective media for ESBL producing bacteria. The identification of strains was obtained with mass spectrometry and the antibiotic susceptibility by disk diffusion in agar media. The ESBL type was determined by PCR. The relative abundance of ESBL-E. coli was measured for positive samples. Eventually, the presence of antibiotics in stool was assessed. Results: The carriage rate of ESBL-E. coli was 57/86 (66.3%). Phenotypic and molecular characterization showed that all ESBL-E. coli strains contained group 1 CTX-M enzymes. Multivariate analysis showed that ESBL-E. coli fecal carriage was associated with the presence of antibiotics in stools (p < 0.05). Although not significant, mean ESBL relative abundance tended to be higher in patients with antibiotic exposure. Conclusions: Our results show that the carriage of ESBL-E. coli fecal carriage in women with UTI suspicion from the Cameroonian community is extremely high and associated with recent antibiotic intake.

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Section: Discussioncontrasting

confidence: 56%

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Djuikoue

Woerther

Toukam

et al. 2016

J Infect Dev Ctries

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“…It has been less frequent among susceptible isolates (13,30,41), but the apparent ease with which it acquired resistance combined with its virulence has fuelled its pandemic spread (13). Except for a few studies (27,40), ST131 has been the dominant clone in most studies on ESBL-producing E. coli (6,13,31,35,42); therefore, its high frequency here was not surprising. The lowest rate in GI is concordant with other reports from Spain and Barcelona (33,43,44); so are the higher rates in France (BM) and Italy (FS) (41,45).…”

Section: Resultsmentioning

confidence: 99%

“…It is easier to understand why just single isolates of ST73 (in FS) and ST95 (TA) were identified. These highly pathogenic clones have frequently been recovered from clinical samples but rarely, if at all, with ESBLs (29,34,(49)(50)(51), which may explain their absence in the analyses targeted on ESBL-positive E. coli isolates (27,31,32,44). MLST versus PFGE.…”

Section: Resultsmentioning

confidence: 99%

Clonal Structure, Extended-Spectrum β-Lactamases, and Acquired AmpC-Type Cephalosporinases of Escherichia coli Populations Colonizing Patients in Rehabilitation Centers in Four Countries

Izdebski

Baraniak

Fiett

et al. 2013

Antimicrob Agents Chemother

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The prospective project MOSAR was conducted in five rehabilitation units: the Berck Maritime Hôpital (Berck, France), Fondazione Santa Lucia (Rome, Italy), Guttmann Institute (GI; Barcelona, Spain), and Loewenstein Hospital and Tel-Aviv Souraski Medical Center (TA) (Tel-Aviv, Israel). Patients were screened for carriage of Enterobacteriaceae resistant to expanded-spectrum cephalosporins (ESCs) from admission until discharge. The aim of this study was to characterize the clonal structure, extendedspectrum ␤-lactamases (ESBLs), and acquired AmpC-like cephalosporinases in the Escherichia coli populations collected. A total of 376 isolates were randomly selected. The overall number of sequence types (STs) was 76, including 7 STs that grouped at least 10 isolates from at least three centers each, namely, STs 10, 38, 69, 131, 405, 410, and 648. These clones comprised 65.2% of all isolates, and ST131 alone comprised 41.2%. Of 54 STs observed only in one center, some STs played a locally significant role, like ST156 and ST393 in GI or ST372 and ST398 in TA. Among 16 new STs, five arose from evolution within the ST10 and ST131 clonal complexes. ESBLs and AmpCs accounted for 94.7% and 5.6% of the ESC-hydrolyzing ␤-lactamases, respectively, being dominated by the CTX-M-like enzymes (79.9%), followed by the SHV (13.5%) and CMY-2 (5.3%) types. CTX-M-15 was the most prevalent ␤-lactamase overall (40.6%); other ubiquitous enzymes were CTX-M-14 and CMY-2. Almost none of the common clones correlated strictly with one ␤-lactamase; although 58.7% of ST131 isolates produced CTX-M-15, the clone also expressed nine other enzymes. A number of clone variants with specific pulsed-field gel electrophoresis and ESBL types were spread in some locales, potentially representing newly emerging E. coli epidemic strains.

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“…Instead, nearly half of them belonged to CCs ST10 and ST58. ST10 and ST58 have both a global distribution, and they have been isolated from humans as well as animals (41)(42)(43)(44). The K. pneumoniae outbreak in Uppsala was also caused by a global clone, ST16 (12), which in recent years has been associated with several clinically important carbapenemases (45).…”

Section: Discussionmentioning

confidence: 99%

Silver Resistance Genes Are Overrepresented among Escherichia coli Isolates with CTX-M Production

Sütterlin

Edquist

Sandegren

et al. 2014

Appl Environ Microbiol

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Members of the Enterobacteriaceae with extended-spectrum beta-lactamases (ESBLs) of the CTX-M type have disseminated rapidly in recent years and have become a threat to public health. In parallel with the CTX-M type expansion, the consumption and widespread use of silver-containing products has increased. To determine the carriage rates of silver resistance genes in different Escherichia coli populations, the presence of three silver resistance genes (silE, silP, and silS) and genes encoding CTX-M-, TEM-, and SHV-type enzymes were explored in E. coli isolates of human (n ‫؍‬ 105) and avian (n ‫؍‬ 111) origin. The antibiotic profiles were also determined. Isolates harboring CTX-M genes were further characterized, and phenotypic silver resistance was examined. The silE gene was present in 13 of the isolates. All of them were of human origin. Eleven of these isolates harbored ESBLs of the CTX-M type (P ‫؍‬ 0.007), and eight of them were typed as CTX-M-15 and three as CTX-M-14. None of the silE-positive isolates was related to the O25b-ST131 clone, but 10 out of 13 belonged to the ST10 or ST58 complexes. Phenotypic silver resistance (silver nitrate MIC > 512 mg/liter) was observed after silver exposure in 12 of them, and a concomitant reduced susceptibility to piperacillin-tazobactam developed in three. In conclusion, 12% of the human E. coli isolates but none of the avian isolates harbored silver resistance genes. This indicates another route for or level of silver exposure for humans than that caused by common environmental contamination. Since silE-positive isolates were significantly more often found in CTX-M-positive isolates, it is possible that silver may exert a selective pressure on CTX-M-producing E. coli isolates.

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Prevalence and characterisation of extended-spectrum beta-lactamase (ESBL)-producing Escherichia coli isolates in healthy volunteers in Tunisia

Cited by 88 publications

References 27 publications

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Intestinal carriage of Extended Spectrum Beta-Lactamase producing E. coli in women with urinary tract infections, Cameroon

Clonal Structure, Extended-Spectrum β-Lactamases, and Acquired AmpC-Type Cephalosporinases of Escherichia coli Populations Colonizing Patients in Rehabilitation Centers in Four Countries

Silver Resistance Genes Are Overrepresented among Escherichia coli Isolates with CTX-M Production

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