Prevalence and Associated Symptoms ofHelicobacter pyloriInfection among Schoolchildren in Kassala State, East of Sudan

Abstract

This study aimed to determine the prevalence of H. pylori infections among schoolchildren and investigate the associations between H. pylori seropositivity and existence of gastrointestinal symptoms. Methods. A prospective cross-sectional study was conducted during a period from January to December 2012 at Kassala state, east of Sudan. Schoolchildren from different primary schools were enrolled in the study. Sociodemographic characteristics and gastrointestinal symptoms were recorded from each child. A rapid immunochromatographic test was performed for the detection of H. pylori IgG antibodies. Data on patient demographic characteristics, clinical diagnosis, and findings of H. pylori infection were analyzed by simple descriptive statistics. Results. Among 431 schoolchildren, H. pylori seropositivity was found to be 21.8%. The majority of children (79; 84%) had BMI below the normal range. The most frequent symptoms associated with H. pylori infections were nausea (25.5%), followed by gastric pain (24.5%) and heart pain (20.2%). There were statistically significant differences in H. pylori seropositivity between boys and girls (p = 0.003). Conclusions. The prevalence of H. pylori infection among schoolchildren in Kassala city has been documented. Although the majority of the disease was associated with several gastrointestinal symptoms, the role of infection in the etiology of abdominal symptoms needs further investigations.

Molecular Phylogenetic Analysis of16S rRNASequences Identified Two lineages ofH. pyloriStrains Detected from Different Regions in Sudan Suggestive of Differential Evolution

“…(59) However, molecular tests and PCR technology have been raised as a useful alternative means of bacterial identification, which may circumvent some of these difficulties (59,60) but has limited mainly by contamination and inadequate sensitivity issues. (61) (18,19,(62)(63)(64)(65) Tetracycline is one of the 30S-targeting antibiotics that inhibit translation elongation by sterically interfering with the binding of aminoacyl-tRNA to the A-site of the ribosome. (66) Therefore, mutations located in two domains (III and IV) in 16S rRNA: helix 34 and the loop next to helix 31 (67) can affect the conformation of the tetracycline-binding site, leading to high-level resistance.…”

prevalence of Helicobacter pylori among Sudanese patients diagnosed with colon polyps and colon cancer using immunohistochemistry technique.

“…In Sudan, no reports addressing this manner were existed. Previous studies investigating the seroprevalence of H. pylori among Sudanese using ELISA and rapid immunochromatographic tests for the detection of H. pylori IgM and IgG antibodies, reported a seroprevalence ranging from 20% up to 70% [32][33][34][35]. Therefore, the aim of this study was to investigate the presence of H. pylori infections among Sudanese patients diagnosed with colon polyps and colon cancer and to correlate between its presence and the type of the lesions.…”

Prevalence of Helicobacter pylori among Sudanese patients diagnosed with colon polyps and colon cancer using immunohistochemistry technique

“…In Sudan, no reports addressing this manner were existed. Previous studies investigating the seroprevalence of H. pylori among Sudanese using ELISA and rapid immunochromatographic tests for the detection of H. pylori IgM and IgG antibodies, reported a seroprevalence ranging from 20% up to 70% [31][32][33][34]. Therefore, the aim of this study was to investigate the presence of H. pylori infections among Sudanese patients diagnosed with colon polyps and colon cancer and to correlate between its presence and the type of the lesions.…”