Prevalence and antimicrobial susceptibility of Salmonella infections in free-range pigs

Gómez-Laguna, J.; Hernández, M.; Creus, E.; Echeita, A.; Otal, Julio; Herrera-León, Silvia; Astorga, R.

doi:10.1016/j.tvjl.2010.09.009

Cited by 29 publications

(16 citation statements)

References 7 publications

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“…This fact represents the first description of Salmonella spp. isolation from mandibular lymph nodes of wild boars, and is similar to previous reports in this species for mesenteric lymph nodes (Gómez‐Laguna et al., ). Regarding tonsils, our study analyses for the first time the presence of Salmonella spp.…”

Section: Discussionsupporting

confidence: 90%

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Molino

Sánchez²,

Risco³

et al. 2019

Transbound Emerg Dis

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Summary The importance of wild boars as game species in Spain is well known. Their feeding habits and intrusive behaviour, together with a progressively wider spreading of populations, increases the interactions of these animals with livestock and humans. Considering that wild boars could have a potential role in the transmission of certain pathogens as salmonellae, the aims of this study were to determine the prevalence of Salmonella spp. in wild boars hunted in central‐western Spain, the occurrence of this pathogen in tonsils, mandibular lymph nodes and faeces (as markers for transmission risk), and to define the phylogenetic relationships among isolated strains, in order to investigate the circulation pathways of bacteria among tissues, animals and estates. Samples from 1,041 hunted wild boars were analysed for the presence of Salmonella spp. by bacteriological culture. Isolates were confirmed by PCR and serotyped in the Spanish national reference laboratory. The genetic relationships between strains were determined by PFGE. The results showed a 7.7% of positive animals (81 wild boars), being tonsils the organ most frequently colonised by Salmonella spp. (18.7%), followed by lymph nodes (5.1%) and faecal samples (2.9%). Serovars Enteritidis and Newport were the most frequent amongst the 34 different serovars obtained. The pulsed‐field gel electrophoresis (PGFE) analysis showed a great genetic diversity, with serovars that exhibited different pulsotypes when isolated from different estates and multiple serovars in the same estate. In conclusion, this study reveals the importance of wild boars as carriers and possible transmitters of virulent and/or antimicrobial‐resistant clones of Salmonella spp. to livestock and humans.

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Section: Discussionsupporting

confidence: 90%

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Molino

Sánchez²,

Risco³

et al. 2019

Transbound Emerg Dis

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“…The high resistance rates of our isolates for ciprofloxacin are similar to those found in pigs from Spain (97.1%) (EFSA 2010). In contrast, studies in other countries revealed minor occurrence of resistant Salmonella strains against tetracycline, sulfonamides and nalidixic (Gomez-Laguna et al 2011, Thai et al 2012. Interestingly, ciprofloxacin, tetracycline and nalidixic acid that showed low effectiveness in our Salmonella strains, are considered antimicrobials of choice to humans and animal therapy of salmonellosis (Lesser & Miller 2005, Radostits et al 2007.…”

Section: Discussionmentioning

confidence: 99%

“…In Spain, studies report Salmonella resistance to streptomycin (46% of tested serovars), tetracycline (30%), sulfonamides (25%) and ampicillin (23%), with 36% of serovars multidrug-resistant (Gomez-Laguna et al 2011). In a similar study conducted in Vietnam, from poultry and pork meat strains, resistance of Salmonella to at least one antimicrobial was found in 78.4% of samples, with 23.2% MDR (Thai et al 2012).…”

Section: Introductionmentioning

confidence: 88%

Frequency, serotyping and antimicrobial resistance pattern of Salmonella from feces and lymph nodes of pigs

et al. 2016

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Resumo.-[Isolamento, sorotipagem e padrões de resistência a antimicrobianos de Salmonella em fezes e linfonodos de suínos.] Nas últimas décadas, o aumento de cepas circulante de Salmonella concomitantemente a resistência microbiana tem despertado a preocupação dos órgãos de Saúde Pública. Deste modo, o objetivo do presente trabalho foi pesquisar a presença de Salmonella a partir de fezes (n=50), linfonodos mediastinos (n=50), mesentéricos (n=50) e submandibular (n=50) oriundos de um abatedouro suíno. As cepas isoladas foram sorotipadas e testadas quanto a resistência antimicrobiana. A presença de Salmonella isolada foram em 10% (20/200) do total de amostras, sendo 20% dos linfonodos submandibulares, 18% dos linfonodos mesentéricos e 2% das fezes. Os sorotipos encontrados foram S. Typhimurium (55%), S. enterica subsp. enterica 4,5,12: i: -(35%), S. Brandenburg (5%) e S. Salmonellosis is a foodborne disease caused by bacteria of the genus Salmonella, being pigs and pork-products potentially important for its occurrence. In recent decades, some serovars of Salmonella have shown increase of resistance to conventional antimicrobials used in human and animal therapy, with serious risks for public health. The aim of this study was to evaluate feces (n=50), mediastinal (n=50), mesenteric (n=50) and mandibular (n=50) lymph nodes obtained from slaughter houses for Salmonella spp. Positive samples were serotyped and subjected to an in vitro antimicrobial susceptibility test, including the extended-spectrum beta-lactamase (ESBL) production. Salmonella species were identified in 10% (20/200) of total samples. From these, 20% (10/50) were identified in the submandibular lymph nodes, 18% (9/50) in the mesenteric lymph nodes, 2% (1/50) in feces and 0% (0/50) in the mediastinal lymph nodes. The serotypes found were Salonella Typhimurium (55%), S. enterica subsp. enterica 4,5,12: i: -(35%), S. Brandenburg and S. Derby with 5% (5% each). All strains showed resistance to at least one antimicrobial; 90% were resistant to four or more antimicrobials, and 15% were multidrug-resistant. Resistance to ciprofloxacin, tetracycline and nalidixic acid was particularly prevalent amongst the tested serovars. Here, we highlighted the impact of pigs in the epidemiological chain of salmonellosis in domestic animals and humans, as well as the high antimicrobial resistance rates of Salmonella strains, reinforcing the necessity for responsible use of antimicrobials for animals as an emergent One Health issue, and to keep these drugs for human therapy approaches.

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“…In this sense, our group recently reported a similar prevalence for Salmonella spp. (isolated from the ileocolic lymph nodes) in pigs in indoor and outdoor systems, but with differences in the main serovars isolated (Gómez‐Laguna et al., ).…”

Section: Discussionmentioning

confidence: 99%

A serological Survey ofBrucellaspp.,Salmonellaspp.,Toxoplasma gondiiandTrichinellaspp. in Iberian Fattening Pigs Reared in Free-Range Systems

Hernández¹,

Gómez-Laguna²,

Tarradas

et al. 2013

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Zoonotic agents such as Brucella spp., Salmonella spp., Toxoplasma gondii and Trichinella spp., all considered high-risk zoonotic pathogens by the European Food Safety Agency (EFSA), may cause no symptoms of infection in free-range pigs yet still have a significant public health impact. A serological survey was therefore performed to determine the history of occurrence of these pathogens in such pigs in southern Spain. A total of 709 serum samples were collected at abattoir from pigs from 79 farms and analysed for specific antibodies against the above pathogens using commercially available ELISA kits. Encysted Trichinella spp. larvae were also sought following the artificial digestion method of diaphragm pillar muscle. The results showed Salmonella spp. to be widely distributed among the sampled herds [73.42%, 95% confidence interval (CI95 ) 65.6-81.78] and Toxoplasma gondii to be present in over half (58.23%, CI95 47.33-69.07). The seroprevalence of Brucella spp. was very low (3.8%, CI95 0.18-7.42), and antibodies against Trichinella spp. were not detected. No encysted Trichinella spp. larvae were microscopically detected.

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Prevalence and antimicrobial susceptibility of Salmonella infections in free-range pigs

Cited by 29 publications

References 7 publications

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Prevalence ofSalmonellaspp. in tonsils, mandibular lymph nodes and faeces of wild boar from Spain and genetic relationship between isolates

Frequency, serotyping and antimicrobial resistance pattern of Salmonella from feces and lymph nodes of pigs

A serological Survey ofBrucellaspp.,Salmonellaspp.,Toxoplasma gondiiandTrichinellaspp. in Iberian Fattening Pigs Reared in Free-Range Systems

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