Pretreatment Reproductive Stage and Oocyte Development Induced by Salmon Pituitary Homogenate in the Japanese Eel &lt;i&gt;Anguilla japonica&lt;/i&gt;

Ijiri, Shigeho; Kayaba, Takaaki; Takeda, Noriko; Tachiki, Hiroyuki; Adachi, Souichi; Yamauchi, Kazuyo

doi:10.2331/fishsci.64.531

Cited by 57 publications

(40 citation statements)

References 3 publications

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“…The females had poor gonad development either at stage I: oogonium or at stage II: previtellogenic oocyte when hormone injections started. These stages do not give good results with a few hormone injections (Ijiri et al 1998). Japanese eels are normally at stage III (cortical alveolus stage) when they start spawning migration.…”

Section: Discussionmentioning

confidence: 99%

“…Significance level: * p \ 0.05; Ns not significant: n = 5 for females in each treatment and n = 3 for males in each treatment. 1 m: weekly treated males; 2 m: biweekly treated males; 1: weekly treated females and males; 2: biweekly treated females and males maturation (Ijiri et al 1998). However, eels show a lot of variation in gonad development, and this might be due to differences in management.…”

Section: Discussionmentioning

confidence: 99%

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Effect of hormone injection frequency on the lipid content and fatty acid compositions in gonad, muscle and liver of Anguilla japonica during artificial maturation

Rupia

Shen

et al. 2013

Aquacult Int

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Two groups of Anguilla japonica were treated with hormone, one on a weekly basis, the other on a biweekly basis. Intramuscular injection was applied at a dose of 0.75 mg/kg BW carp pituitary extract (CPE) plus 150 IU/kg BW human chorionic gonadotropin (hCG) for female fish, while males received half of this dose. The average total lipid content of the gonads from the weekly treated group, i.e. 20.6 ± 1.2 % for females and 18.0 ± 2.3 % for males, was significantly higher than the biweekly treated group, 16.8 ± 0.7 and 15.5 ± 1.3 %, respectively (p \ 0.05). For both muscle and liver, the readings were not significantly different. The gonads from the weekly treated fish had more fatty acids, particularly saturated fatty acids, polyunsaturated fatty acids, highly unsaturated fatty acids, eicosapentaenoic acid (20:5n-3, EPA), docosahexaenoic acid (22:6n-3, DHA) and arachidonic acid (20:4n-6, ARA). Histological examination showed that the ovaries of both the weekly and the biweekly treated fish were mainly at stage IV. However, the weekly treated females had bigger oocyte diameter (722.0 ± 60.9 lm) than the biweekly females (611.6 ± 22.6 lm). These results suggest that CPE and hCG promoted the maturation process for both scheduled induction and that the frequency of hormone injection influenced the biochemical composition of gonads, especially their lipids. Our study describes for the first time the effect of hormone injection frequency on the lipid content and fatty acid composition in the gonads of A. japonica during artificial maturation.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Effect of hormone injection frequency on the lipid content and fatty acid compositions in gonad, muscle and liver of Anguilla japonica during artificial maturation

Rupia

Shen

et al. 2013

Aquacult Int

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“…Japanese eels (A. japonica) were obtained from an eel farm, feminized in the glass eel stage (Ijiri et al 1998), and reared on commercial dry feeds. Figure 8 Growth differentiation factor-9 (Gdf9) mRNA levels in ovarian tissue of juvenile eel, Anguilla japonica, after treatment with saline (ctrl) or 500 ng/g high (hi GH) or low molecular weight recombinant eel GH (lo GH) for 12 days.…”

Section: Animalsmentioning

confidence: 99%

Effects of reproductive stage, GH, and 11-ketotestosterone on expression of growth differentiation factor-9 in the ovary of the eel, Anguilla australis

Lokman¹,

Kazeto²,

Ozaki³

et al. 2010

Reproduction

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In order to study the regulation of the growth differentiation factor-9 (gdf9) gene in a primitive teleost with semelparous life history, we cloned a cDNA encoding shortfinned eel Gdf9, expressed a partial peptide in Escherichia coli, and raised an antiserum to evaluate changes in Gdf9 expression during its pituitary homogenate-induced reproductive cycle. The effects of in vivo and in vitro exposure to the androgen 11-ketotestosterone (11-KT), known to affect previtellogenic (PV) oocyte growth, were also determined. Furthermore, we investigated whether Gdf9 expression was metabolically gated by treating PV fish with recombinant GH in vivo. Immunoreactive proteins of ca. 52 and 55 kDa were identified by western blot analysis. Gdf9 message and protein were most abundant in PV oocytes, and peaked slightly earlier for mRNA than for protein. Captivity resulted in reduced gdf9 mRNA levels, which were restored following pituitary homogenate treatment. As oocytes progressed through induced oogenesis, Gdf9 expression decreased. Neither 11-KT nor GH treatment affected gdf9 mRNA levels in PV fish, although GH could partially restore handling-or captivity-induced decreases in gdf9 mRNA levels. Semelparous eels thus show an expression pattern of Gdf9 during oogenesis that is similar to that seen in other vertebrates, that appears responsive to handling or captivity stress, and whose control remains to be elucidated.

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“…Forty-three eels feminized by estradiol-17β administration [21] were artificially induced with salmon pituitary homogenate (SPH) and ovarian tissues at various developmental stages were collected. Two further eels served as Ringer-injected controls.…”

Section: Animal Treatment Tissue Collection and Rna Extractionmentioning

confidence: 99%

Cloning and characterization of a cDNA encoding cholesterol side-chain cleavage cytochrome P450 (CYP11A1): Tissue-distribution and changes in the transcript abundance in ovarian tissue of Japanese eel, Anguilla japonica, during artificially induced sexual development

Kazeto

Ijiri

Adachi

et al. 2006

The Journal of Steroid Biochemistry and Molecular Biology

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Pretreatment Reproductive Stage and Oocyte Development Induced by Salmon Pituitary Homogenate in the Japanese Eel <i>Anguilla japonica</i>

Abstract: Induction of the ovarian development was investigated in three groups of eels with respect to the importance of the pretreatment reproductive stage. Initially, the developmental stages of oocytes varied

Cited by 57 publications

References 3 publications

Effect of hormone injection frequency on the lipid content and fatty acid compositions in gonad, muscle and liver of Anguilla japonica during artificial maturation

Effect of hormone injection frequency on the lipid content and fatty acid compositions in gonad, muscle and liver of Anguilla japonica during artificial maturation

Effects of reproductive stage, GH, and 11-ketotestosterone on expression of growth differentiation factor-9 in the ovary of the eel, Anguilla australis

Cloning and characterization of a cDNA encoding cholesterol side-chain cleavage cytochrome P450 (CYP11A1): Tissue-distribution and changes in the transcript abundance in ovarian tissue of Japanese eel, Anguilla japonica, during artificially induced sexual development

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